Determination of N-acetylcysteine, intact and oxidized, in plasma by column liquid chromatography and post-column derivatization

Johansson, Margareta; Westerlund, Douglas

doi:10.1016/s0021-9673(01)94649-7

Cited by 35 publications

(13 citation statements)

References 42 publications

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“…We examined the optimum types and concentrations of organic solvents (methanol, ethanol, acetonitrile, and tetrahydrofuran; 2 -25% (v/v)) and additives (acetic acid, boric acid, EDTA·2Na, The selected optimum derivatization parameters described in the Experimental section were almost similar to those of previous postcolumn NPM methods. [23][24][25][26] However, in the analysis of polythiols, the selectivity and sensitivity were greatly improved because the emission wavelength observed in the present method was red-shifted by ca. 100 nm, as compared to that of previous monothiol analysis methods.…”

Section: Optimization Of Derivatization and Separationmentioning

confidence: 87%

“…1). NPM is one of the most popular fluorogenic reagents for the LC analysis of thiols used in both postcolumn [23][24][25][26] and precolumn reactions; [27][28][29][30][31] however, excimer fluorescence detection has not been carried out until now. Dimercaprol (BAL) and dithiothreitol (DTT) were used as model polythiols to optimize the separation and derivatization conditions.…”

Section: Introductionmentioning

confidence: 99%

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Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

et al. 2009

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An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440 -520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.

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Section: Optimization Of Derivatization and Separationmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

et al. 2009

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“…5b) was also reported [19,40,42]. Because NPM is unstable in aqueous solution, the postcolumn reagent has been prepared in a water miscible organic solvent (i.e., acetonitrile).…”

Section: Maleimide Labelingmentioning

confidence: 95%

Molecular and nanoparticle postcolumn reagents for assay of low-molecular-mass biothiols using high-performance liquid chromatography

2009

Journal of Chromatography B

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“…The reagent pyrenemaleimide provided derivatization of N -acetylcysteine with a detection limit of 10 pmol. (320) Postcolumn complexation of disulfiram and two of its metabolites using Cu 2+ allowed colorimetric detection at 435 nm with detection limits in the low parts-per-billion range. (321) Quenched peroxalate CL has been employed using immobilized reagents in a postcolumn reactor for thioridazin, sulforidazine, and methimazole.…”

Section: Sulfur Compoundsmentioning

confidence: 99%

Chemical Reagents and Derivatization Procedures in Drug Analysis

Danielson

Gallagher

Bao

2008

Encyclopedia of Analytical Chemistry

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This article describes both pre‐ and postcolumn derivatization chemistry used in conjunction with either chromatography or capillary electrophoresis (CE) to facilitate the determination of drugs. Generally, only prederivatization is used in gas chromatography (GC), principally to enhance the volatility, temperature stability, and/or detectability. The GC section considers derivatization of drugs by reagent class: alkylation, acylation, and silylation. The GC sample‐handling section describes an approach that combines the extraction and derivatization steps together. Both pre‐ and postcolumn derivatization are common approaches for high‐performance liquid chromatography (HPLC) and many of these methods have been adapted for CE. Derivatization for HPLC is often directed toward aliphatic amines, carboxylic acids, or alcohols that are difficult to detect at low levels by absorbance, luminescence, or electrochemical means. In addition, small hydrophilic molecules upon prederivatization are often converted into larger more hydrophobic compounds, making reversed‐phase HPLC easier or even feasible. The HPLC section considers derivatization of drugs based on the following types: alkaloids, amines, antibiotics, barbiturates, carbonyl compounds and carboxylic acids, catecholamines, hydroxy compounds, steroids, and sulfur compounds. For the GC and HPLC sections, tables are included, giving the structures of the more important derivatizing agents, the analytes, and the corresponding reaction products. A brief rationale for derivatization chemistry with CE concludes this article. For CE, derivatization is often done to improve detectability as the path length for absorbance detection is very short and fluorescence can be effective using laser‐based systems.

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Determination of N-acetylcysteine, intact and oxidized, in plasma by column liquid chromatography and post-column derivatization

Cited by 35 publications

References 42 publications

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

Molecular and nanoparticle postcolumn reagents for assay of low-molecular-mass biothiols using high-performance liquid chromatography

Chemical Reagents and Derivatization Procedures in Drug Analysis

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