Determination of N-acetylglucosamine in cosmetic formulations and skin test samples by hydrophilic interaction liquid chromatography and UV detection

Pedrali, Alice; Bleve, Mariella; Capra, P; Jonsson, Tobias; Massolini, Gabriella; Perugini, Paola; Marrubini, Giorgio

doi:10.1016/j.jpba.2014.12.014

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…It is worthy to note, that for all COS analyzed in this study, α anomers showed shorter retention times than β isomers indicating a weaker interaction of α anomers with the stationary phase. For each COS, the anomer/peak assignment was based on the peak area integration ratio of 2:1 assigned for α: β anomers, respectively, as found in the literature for D-glucosamine, [48,49] which is also in agreement with the α: β peak integration ratio calculated from 1 H NMR spectra of fully acetylated and deacetylated COS (Supplementary Information Fig S1 -S3).…”

Section: Column Equilibration Under Acidic Conditionssupporting

confidence: 59%

Impact of HILIC Amino-Based Column Equilibration Conditions on the Analysis of Chitooligosaccharides

2022

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Obtaining well-defined Chitooligosaccharides (COS) structures is very important as their physicochemical and biological properties depends strongly on their degree of polymerization (DP) and degree of N-acetylation (DA).Hydrophilic Interactions Liquid Chromatography (HILIC), that is commonly used for COS analyses, suffers from several drawbacks, related to their complex structure, leading to broadening/splitting of the chromatogram signals.We investigated, herein, on the role of the equilibration step of an amino-based HILIC column on the separation of COS DP≤ 5. We demonstrated that COS could be separated according to their DP after equilibrating the column by a NaHCO3 buffer solution (100 mM, pH=10) and mild elution chromatographic parameters (neutral mobile phase, r.t.); or according to their DA after equilibrating the column by an NH4Ac buffer solution (50 mM, pH=4.5).Also, the nature of the counterion of the column stationary phase was found to affect both retention times and signal profiles of analyzed COS.

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Section: Column Equilibration Under Acidic Conditionssupporting

confidence: 59%

Impact of HILIC Amino-Based Column Equilibration Conditions on the Analysis of Chitooligosaccharides

2022

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“…N-acetylglucosamine (GlcNAc), an amino-sugar and a building block of the fungal polysaccharide chitin, was present only in HUM (26 mg/g of the extract) as it is a product of chitinolysiswhich occurs during maturation of the fruiting bodies (Lim and Choi, 2009). Its presence was specifically targeted by NMR, as it's not commonly found Importance of hyaluronic acid in the skin's health and integrity prompted the potential use of GlcNAc in products for wound healing and wrinkle treatment (Pedrali et al, 2015). In addition, GlcNAc was shown to inhibit tyrosinase by inhibiting tyrosinase glycosilation, thus preventing activation of the enzyme and production of melanin (Ortonne and Bissett, 2008), so it was proposed that it could be used in the treatment of hyperpigmentation caused by aging (Pedrali et al, 2015).…”

Section: Table1mentioning

confidence: 99%

From mycelium to spores: A whole circle of biological potency of mosaic puffball

Petrović

Vunduk

Klaus

et al. 2019

South African Journal of Botany

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Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis -current name is a subject of debate), from three different stages -mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R).Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 μg/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and nonlovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits.Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), αtocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.

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“…Previously, GlcNAc has been used in pharmaceutical formulations as delivery tool. 51,60,75,76 GlcNAc incorporated in CaPNP/ multipeptide formulation is expected to act as a danger signal for the immune system, which often plays a role in initiation of the immune recognition but not necessarily the immune response. 77 Activation of innate immune response is considered very important so that it can prime the immune system that involves APCs activation.…”

Section: Discussionmentioning

confidence: 99%

A novel immunization approach for dengue infection based on conserved T cell epitopes formulated in calcium phosphate nanoparticles

Huang

Karabudak

Comber

et al. 2017

Human Vaccines & Immunotherapeutics

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Dengue virus (DV) is the etiologic agent of dengue fever, the most significant mosquito-borne viral disease in humans. Most DV vaccine approaches are focused on generating antibody mediated responses; one such DV vaccine is approved for use in humans but its efficacy is limited. While it is clear that T cell responses play important role in DV infection and subsequent disease manifestations, fewer studies are aimed at developing vaccines that induce robust T cells responses. Potent T cell based vaccines require 2 critical components: the identification of specific T cell stimulating MHC associated peptides, and an optimized vaccine delivery vehicle capable of simultaneously delivering the antigens and any required adjuvants. We have previously identified and characterized DV specific HLA-A2 and -A24 binding DV serotypes conserved epitopes, and the feasibility of an epitope based vaccine for DV infection. In this study, we build on those previous studies and describe an investigational DV vaccine using T cell epitopes incorporated into a calcium phosphate nanoparticle (CaPNP) delivery system. This study presents a comprehensive analysis of functional immunogenicity of DV CaPNP/multipeptide formulations in vitro and in vivo and demonstrates the CaPNP/multipeptide vaccine is capable of inducing T cell responses against all 4 serotypes of DV. This synthetic vaccine is also cost effective, straightforward to manufacture, and stable at room temperature in a lyophilized form. This formulation may serve as an effective candidate DV vaccine that protects against all 4 serotypes as either a prophylactic or therapeutic vaccine.

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Determination of N-acetylglucosamine in cosmetic formulations and skin test samples by hydrophilic interaction liquid chromatography and UV detection

Cited by 12 publications

References 19 publications

Impact of HILIC Amino-Based Column Equilibration Conditions on the Analysis of Chitooligosaccharides

Impact of HILIC Amino-Based Column Equilibration Conditions on the Analysis of Chitooligosaccharides

From mycelium to spores: A whole circle of biological potency of mosaic puffball

A novel immunization approach for dengue infection based on conserved T cell epitopes formulated in calcium phosphate nanoparticles

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