Determination of nafcillin and methicillin by different spectrofluorimetric techniques

Pulgarı́n, José A. Murillo; Molina, Aurelia Alañón

doi:10.1016/0039-9140(94)80164-9

Cited by 18 publications

(9 citation statements)

References 7 publications

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“…Several methods for resolving binary mixtures of these penicillins using first derivative spectrofluorimetry, first derivative constant wavelength synchronous luminescence spectrometry and constant energy synchronous luminescence spectrometry were used [130].…”

Section: Nonchromatographic Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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Natural penicillin (benzylpenicillin) is the oldest antibiotic observed by Alexander Fleming in 1928. To broaden its spectrum of activity, natural penicillin was modified, giving rise to a group of antibiotics under the name 'penicillins'. Although an increasing number of bacteria appear to be resistant to them, penicillins are used to treat a variety of bacterial infections including Gram-positive, Gram-negative aerobic and anaerobic bacteria. Consequently, they are widely used in human and veterinary medicine to prevent and treat diseases. This review covers the analytical methodologies, mainly chromatographic, employed to the penicillins determination in pharmaceutical formulations, biological fluids and in production-scale fermentations reported in the literature. Results of published assays are comparatively presented focusing on sample preparation regarding isolation and purification, chromatographic conditions and method validation. Information on chemical structure, spectrum of activity and action mechanism of common penicillins has also been given.

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Section: Nonchromatographic Techniquesmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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“…Derivative and synchronous fluorescence spectrometry are among the most popular techniques. [ 46,47 ] Despite recording emission spectra in fluorescence spectrophotometry by measuring emission over a range of wavelengths at fixed excitation wavelength, synchronous fluorescence spectroscopy (SFS) is based on simultaneous scanning of the excitation and emission monochromators, keeping a constant wavelength difference between excitation and emission wavelengths; Δλ. [ 48 ] Synchronous spectra are sharper than fundamental emission spectra.…”

Section: Introductionmentioning

confidence: 99%

Micelle‐enhanced spectrofluorimetric determination of diphenhydramine: application to human plasma and its simultaneous determination with naproxen in pharmaceutical tablets

2021

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Two simple and sensitive spectrofluorimetric methods were developed and validated for determination of diphenhydramine. The use of sodium dodecyl sulfate surfactant at pH 7 enhances the fluorescence intensity of diphenhydramine at 286 nm (method I) enabling its nanodetermination in biological samples with mean per cent recovery ± SD of 100.33 ± 1.519. Method I was validated according to ICH‐Q2R1 guidelines and was successfully applied for determination of diphenhydramine in pharmaceutical dosage form and spiked human plasma in the concentration ranges 0.1–4.0 μg/mL and 0.2–1.0 μg/mL, respectively. Method I acted as a basis for the development of a first derivative synchronous spectrofluorimetry (method II) for simultaneous analysis of diphenhydramine and naproxen using a zero‐crossing approach. Method II determines both drugs with linearity ranges of 0.05–3.0 μg/mL and 0.1–0.9 μg/mL for diphenhydramine and naproxen, respectively. The developed method was applied for the simultaneous determination of both drugs in their laboratory‐prepared mixtures containing all expected excipients. Method II determines both drugs with a mean percent recovery ± SD of 100.56 ± 0.891 and 100.20 ± 1.125 for diphenhydramine and naproxen, respectively. The method was statistically compared with a reported method using Student's t‐ and F‐ tests, and no significant differences were observed.

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“…Synchronous fluorimetry has been discussed science 1982 [31]. The measurement depends on synchronized scanning of both excitation and emission monochromators either at constant wavelength difference (Δλ) or constant energy difference [31][32][33]. This technique has many advantages over the conventional fluorimetry such as: decrease in spectral difficulty, spectral overlap and increase in peak sharpness [32,33].…”

Section: Bis(nndimethylcarbamoyloxy)mentioning

confidence: 99%

“…The measurement depends on synchronized scanning of both excitation and emission monochromators either at constant wavelength difference (Δλ) or constant energy difference [31][32][33]. This technique has many advantages over the conventional fluorimetry such as: decrease in spectral difficulty, spectral overlap and increase in peak sharpness [32,33]. Coupling mathematical methods with fluorimetry also improves the spectral features of multi-components mixtures with overlapped bands.…”

Section: Bis(nndimethylcarbamoyloxy)mentioning

confidence: 99%

Novel chemo-fluorimetric methods for determination of bambuterol hydrochloride in presence of the active metabolite terbutaline

El‐Din

2019

Journal of advanced Biomedical and Pharmaceutical Sciences

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Three novel, simple, rapid and sensitive spectrofluorimetric methods were developed for the simultaneous determination of bambuterol hydrochloride (BAM) in presence of its active metabolite and acidic degradation product; terbutaline (TER). Method I based upon calculating the first derivative values (D 1 ) of the previously stored conventional emission spectra of both drugs. BAM and TER were measured at 302 and 286 nm respectively. Methods II & III built upon estimating the sum amount of both drugs at the isosbestic points (287.5 nm in the conventional emission method and 229.5, 247.5 nm in synchronous emission method). TER was determined using the previously mentioned D 1 methods while BAM concentrations were considered by subtraction. All the optimization parameters were carefully optimized such as Δ λ and diluting solvents. The calibration curves were rectilinear over the range of 0.4 -8.0 µg mL -1 with quantitation limits ranged from 0.246 -0.273 µg mL -1 . The proposed methods were successfully applied to the assay of synthetic laboratory mixtures, commercially available tablets as well as forced acidic stability testing.

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Determination of nafcillin and methicillin by different spectrofluorimetric techniques

Cited by 18 publications

References 7 publications

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Micelle‐enhanced spectrofluorimetric determination of diphenhydramine: application to human plasma and its simultaneous determination with naproxen in pharmaceutical tablets

Novel chemo-fluorimetric methods for determination of bambuterol hydrochloride in presence of the active metabolite terbutaline

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