Heavy metals and antibiotics resistance are intense public interest owing to their pollution in the environment and potential deleterious effects on human health. A total of 15 isolates of Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli (50 manure garden soils) were tested for their resistance against certain heavy metals and antibiotics. Minimum inhibitory concentrations of Pb 2+ , Co 2+ , Cu 2+ , and Zn 2+ for each isolate were also determined. B. subtilis, B. cereus, P. aeruginosa and E. coli exhibited high tolerance to minimum inhibitory concentrations of 3,200 µg/ml for Pb 2+ , 400 µg/ml for Cu 2+ and Co 2+ , and 100 µg/ml for Zn 2+ in agar plates. All the isolates were highly resistant to lead and they showed 100% growth in 3,200 µg/ml concentration. It was observed that all isolates were resistant to a wide range of antibiotics namely clindamycin, ceftazidime, aztreonam and vancomycin.