Objectives: This research focuses on the determination of the natural radionuclides radium, thorium, and potassium in the twenty-six sediment samples collected at the sea, beach, and creek regions of Ennore Port. Methods: The activity concentrations of 226Ra, 232Th, and 40K were determined using gamma ray spectrometry with a high-purity germanium (HPGe) detector. Findings: The average activity concentrations of 226Ra, 232Th, and 40K were in the descending order of 40K (397.58 Bq kg-1) > 232Th (65.83 Bq kg-1) > 226Ra (18.28 Bq kg-1). The estimated average values of radiological parameters such as radium equivalent activity (143.04 Bq kg-1), absorbed dose rate (64.91 nGy h-1), annual effective dose equivalent (0.32 mSv y-1), and external hazard index (0.39) were lower than the respective world average values, reported by United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR, 2000). Moreover, the representative level index and annual gonadal dose equivalent were slightly higher than the world average value. Hence, this research proved that the study area is radiologically safe for humans and the environment. Novelty: A location and sample collection-based novelty is approached to carried out the work. Sea sediments were also collected along with samples from creek and beach regions in order to examine the dispersion of natural radionuclides from land to marine environments. The samples from the beach and creek regions were collected using a Peterson grab sampler. Especially in the sea region, the samples were collected using a Van Veen grab sampler at a depth of 4 m and a distance of 10 m parallel to the shoreline. Keywords: Natural radioactivity, Sediment, Ennore, Gamma ray spectrometry, HPGe detector, Radiological parameters