Determination of naturally occurring MTBE biodegradation by analysing metabolites and biodegradation by-products

Martienssen, Marion; Fabritius, Holger; Kukla, S.; Balcke, Gerd Ulrich; Hasselwander, Eyk; Schirmer, Mario

doi:10.1016/j.jconhyd.2006.04.007

Cited by 51 publications

(27 citation statements)

References 26 publications

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“…Nitrous oxide was sampled from the headspace using a gas-tight syringe and detected by a GC-ECD system (Shimadzu, 60°C isotherm, packed column Haye Q 80/100 mesh, Vici International) with valve injection using a 1 ml injection coil. Ammonia was analyzed as described by Martienssen et al (2006).…”

Section: Chemical Analysesmentioning

confidence: 99%

Biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions

et al. 2007

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Pseudomonas veronii strain UFZ B549, Acidovorax facilis strain UFZ B530, and a community of indigenous groundwater bacteria, adapted to oxygen limitation, were cultivated on chlorobenzene and its metabolites 2-chloro-cis,cis-muconate and acetate/succinate under hypoxic and denitrifying conditions. Highly sensitive approaches were used to maintain defined low oxygen partial pressures in an oxygen-re-supplying headspace. With low amounts of oxygen available all cultures converted chlorobenzene, though the pure strains accumulated 3-chlorocatechol and 2-chloro-cis,cis-muconate as intermediates. Under strictly anoxic conditions no chlorobenzene transformation was observed, while 2-chloro-cis,cis-muconate, the fission product of oxidative ring cleavage, was readily degraded by the investigated chlorobenzene-degrading cultures at the expense of nitrate as terminal electron acceptor. Hence, we conclude that oxygen is an obligatory reactant for initial activation of chlorobenzene and fission of the aromatic ring, but it can be partially replaced by nitrate in respiration. The tendency to denitrify in the presence of oxygen during growth on chlorobenzene appeared to depend on the oxygen availability and the efficiency to metabolize chlorobenzene under oxygen limitation, which is largely regulated by the activity of the intradiol ring fission dioxygenase. Permanent cultivation of a groundwater consortium under reduced oxygen levels resulted in enrichment of a community almost exclusively composed of members of the beta-Proteobacteria and Bacteroidetes. Thus, it is deduced that these strains can still maintain high activities of oxygen-requiring enzymes that allow for efficient CB transformation under hypoxic conditions.

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Section: Chemical Analysesmentioning

confidence: 99%

Biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions

et al. 2007

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“…The hydrogeological and geochemical structure of this site has been investigated in great detail [1]. The main aquifer thickness is 2-4 m and the groundwater table is located approximately 3-4 m below the ground surface.…”

Section: Open Accessmentioning

confidence: 99%

“…The drinking water standard is 5 g/L and this is the concentration that must be achieved at the drinking water wells of the water production plant (VII) located 1.5 km downstream. Reduction of the concentration from 200 to 5 g/L will be achieved through dilution and the natural attenuation capability of the downstream aquifer (for more details see [1] and [5]). The remaining risks can be reduced by additional measures.…”

Section: Technical Solutions For Reducing Mtbe Contaminationmentioning

confidence: 99%

“…In addition to the detailed investigations of the aquifer characteristics required to define the design criteria for the different water collection, treatment and injection facilities, the large-scale research project METLEN was initiated to explore the natural and enhanced natural attenuation potential of MTBE within the aquifer [1]. The project included the development of a pilot-scale on-site treatment facility for MTBE.…”

Section: Other Technical/biological Remediation Optionsmentioning

confidence: 99%

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Sustainable Technologies and Social Costs for Eliminating Contamination of an Aquifer

Schirmer

Niemes

2010

Sustainability

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This case study deals with long-term contamination of the Leuna aquifer, which is intended to be restored using sustainable technologies financed by the state. The contamination can only be solved using active rather than passive intervention, because the aquifer has an extraordinarily low natural attenuation capacity for the specific pollutants. Due to the longevity of the contamination source, the groundwater treatment technology that was chosen for the site must operate for a minimum of 20 years but probably much longer. Since the polluter-pay principle cannot be applied, the estimated dynamic primary remediation costs must be accepted as a political or social cost, which must be paid by current and future generations.

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“…The release of MTBE to the environment, mainly from damaged gasoline underground storage tanks or distribution systems spills, has provoked extended groundwater pollution [2]. Solubility of MTBE in water is approximately 50,000 mg/L at 25˚C and has a low organic carbon-based partitioning coefficient (K OC ) of 11 cm 3 /g resulting in minimal sorption and retardation in natural aquifer [3]. MTBE has a higher vapor pressure than benzene, its Henry's Law constant (0.022 at 25˚C) is 1/10 th benzene.…”

Section: Introductionmentioning

confidence: 99%

Microbial Community from MTBE-Contaminated Soil for Aerobic Biodegradation of MTBE

Montazeri¹,

Sarrafzadeh²

2016

GEP

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This Methyl tert-butyl ether (MTBE) is one of the main additives in gasoline to increase octane rating and consequently reduce air pollution. The physico-chemical properties of this substance (high water solubility, low sorption in soil) result in high mobility and considerable concentrations in aquifers. In this survey, Isfehan Refinery that was encountered with MTBE contamination problem was selected as a case study and the MTBE degradation ability of this contaminated area by its indigenous microorganisms was investigated. In the first step of this survey, the influence of various factors on the aerobic degradation of MTBE such as mixed culture type, incubation time, microbial culture and optimal concentration of MTBE were investigated in shaking flasks and the most important factors were specified by means of fractional factorial design ½. In the second stage by using optimal values which obtained from the first stage, the effects of co-substare parameter and inoculum parameter were assayed by means of response surface method. The results of the experiments showed that the mixed culture type and initial concentration of MTBE were the most significant factors. The results of the experiments showed that the mixed indigenous culture acted better than activated sludge. The initial concentration of MTBE was also one of the most significant factors. At the best condition about 31 percent of MTBE was treated by co-substrating with n-hexane in a ratio of 0.2.

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Determination of naturally occurring MTBE biodegradation by analysing metabolites and biodegradation by-products

Cited by 51 publications

References 26 publications

Biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions

Biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions

Sustainable Technologies and Social Costs for Eliminating Contamination of an Aquifer

Microbial Community from MTBE-Contaminated Soil for Aerobic Biodegradation of MTBE

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