Determination of naturally occurring progestogens in bovine milk as their oxime derivatives using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Regal, Patricia; Nebot, Carolina; Vázquez, Beatriz; Cepeda, Alberto; Fente, C.

doi:10.1002/jsfa.3990

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Most of the works reported in the literature for the analysis of steroids hormones in milk employed HPLC‐MS . There is quite limited information about the separation of steroid hormones by HPLC‐UV and the works previously reported in the literature present the separation of only a limited number of compounds .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mesoporous silicas functionalized with C18 groups as stationary phases for the solid‐phase extraction of steroid hormones in milk

et al. 2014

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In this work, two mesoporous silicas functionalized with C18 groups (SM-C18 and SBA-15-C18) have been synthesized for their evaluation as stationary phases in SPE for the extraction and preconcentration of seven steroid hormones (estrone, estradiol, estriol, ethinyl estradiol, diethylstilbestrol, testosterone, and progesterone ) from milk. The characterization of both materials by diverse techniques such as transmission electron microscopy, SEM, thermogravimetric analysis, X-ray diffraction, and adsorption-desorption isotherms showed that the mesoporous silicas had a high surface area, high pore volume, and a homogeneous distribution of the pores and that both silicas presented a similar degree of functionalization. An analytical methodology for the simultaneous separation of the seven selected steroids by HPLC-DAD was developed. Both silicas were evaluated as stationary phases in SPE for the extraction of the steroid hormones from milk. This HPLC-DAD method was applied to the analysis of all extracts obtained in the SPE experiments, showing that from the two synthesized mesoporus silicas, SBA-15-C18 silica enabled the extraction of the seven compounds with recoveries between 88 and 108% for all except for estriol, for which a recovery of 62% was obtained. The analytical characteristics of this methodology were evaluated, showing good precision and linearity (R2 > 0.994) for all analytes. The comparison of the results obtained with this silica and those obtained with commercial C18 particles and with some other commercial cartridges usually employed in the extraction of steroids showed that SBA-15-C18 silica was able to extract the seven steroids with higher recovery values.

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Section: Introductionmentioning

confidence: 99%

Evaluation of mesoporous silicas functionalized with C18 groups as stationary phases for the solid‐phase extraction of steroid hormones in milk

et al. 2014

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“…Natural and synthetic estrogens have been characterized by the largest endocrine disrupting potential, as confirmed by both in vitro and in vivo studies [2]. The relation between estrogens and several human health problems has been previously reported, such as prostate and breast cancer, perturbation of human reproduction, and endocrine disruption on humans and wildlife [3]. Estrone, estradiol, and estriol are three main natural estrogenic hormones existing in the human body.…”

Section: Introductionmentioning

confidence: 94%

Chemiluminescence behavior of CdTe-hydrogen peroxide enhanced by sodium hypochlorite and sensitized sensing of estrogens

Liu

et al. 2014

Nanoscale Res Lett

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“…20,28 Recent literature has focused more on estrogens and phytoestrogens from specific food sources, particularly dairy. [29][30][31][32][33][34][35] Therefore, while limitations may exist for estimating absolute hormone contents in foods from these studies, the relative amounts of hormones and hormone-like substances between foods can be assessed. Due to their comprehensive nature, their assessment of foods common in the Western diet, and their well-defined methodology, the data presented in these two publications were used to develop estimations of levels of select hormones or hormone-like substances found in 100 g portions (Table 2) or serving sizes (Table 3) of typical foods consumed in the United States.…”

Section: Animal Hormone-like Substances Found In Plantsmentioning

confidence: 99%

Naturally occurring hormones in foods and potential health effects

Palacios

Cortes²,

Jenks³

et al. 2020

Toxicology Research and Application

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Hormones and hormone-like substances, for example, phytoestrogens, are food components that can be endogenously produced by a food source or occur secondary to farming practices. The hormone content of foods has been studied for decades, and safety evaluations in the United States and Europe indicate that naturally occurring hormones found in foods are safe for human consumption. More recent studies have focused on the role of certain hormones found in specific foods (e.g. dairy or soy) and their potential health effects. However, limited summaries exist on food content of hormones and hormone-like phytoestrogens in the context of a comprehensive US diet and implications, if any, of their daily consumption for overall health. This review provides an outline of hormone biosynthesis and functions in the body; discusses the more commonly studied, naturally occurring hormones in food and their biological role within food; estimates relative dietary contribution and when available, bioavailability, of naturally occurring food hormones; and summarizes the potential health associations of their intake in food. Based on the review of the scientific literature, the hormone content of typical serving sizes of commonly consumed foods is undetectable or in quantities that fall well within safety guidelines without any evidence for adverse effects on health.

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Determination of naturally occurring progestogens in bovine milk as their oxime derivatives using high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Abstract: This method has been successfully used in real samples. It is fast and easy-handling and provides a useful tool for the assessment of progestogens in bovine milk.

Cited by 9 publications

References 39 publications

Evaluation of mesoporous silicas functionalized with C18 groups as stationary phases for the solid‐phase extraction of steroid hormones in milk

Evaluation of mesoporous silicas functionalized with C18 groups as stationary phases for the solid‐phase extraction of steroid hormones in milk

Chemiluminescence behavior of CdTe-hydrogen peroxide enhanced by sodium hypochlorite and sensitized sensing of estrogens

Naturally occurring hormones in foods and potential health effects

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