Determination of nicotine and cotinine in rat plasma by liquid chromatography–tandem mass spectrometry

Stolker, Alida Linda A M; Niesing, Willem; Hogendoorn, E.A.; Rambali, A Bisoen; Vleeming, W.

doi:10.1016/j.chroma.2003.08.056

Cited by 41 publications

(44 citation statements)

References 7 publications

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“…The 100u loss for the former ion was due to loss of N-methyl piperazine as has been observed for olanzapine [74] with the unsaturated ring sysyem being resistant to fragmentation. The LOQ A method has been developed for the determination of nicotine and cotinine in rat plasma samples originating from nicotine exposure studies [76]. Nicotine and cotinine were extracted from plasma samples with dichloromethane and concentrated to minimum volume with a nitrogen stream.…”

Section: Other Drugs With Nitrogen-containing Saturated Ring Structuresmentioning

confidence: 99%

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

Smyth¹,

Brooks

2004

Electrophoresis

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A critical review of applications for the period 2000-2003, taken from the Web of Knowledge database, of the techniques high performance liquid chromatography-electrospray ionisation-mass spectrometry (HPLC-ESI-MS) and capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) to the detection and determination of small molecules of significance in clinical and forensic science is presented. The molecules of mass less than 500 Da are chosen according to selected structural classes in which they give ESI signals primarily as [M+H](+) ions although other ions such as [M-H](-), [M+Na](+) and [M+NH(4)](+) are also reported. The structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, carbohydrates, benzimidazoles, other heterocycles, sulphonylureas, anthracyclines, sulphonamides, penicillins, cephalosporins, tetracyclines, nitrocatechols, steroids, flavonoids, oxazaphosphorines, cannabinols, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole and time-of-flight mass spectrometers. The review then gives a critical evaluation of these recent HPLC-ESI-MS and CE-ESI-MS analytical methods for the detection and determination of small molecules of clinical and forensic significance. Analytical information on, for example, sample concentration techniques, HPLC and CE separation conditions, recoveries from biological media and limits of detection (LODs) are provided.

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Section: Other Drugs With Nitrogen-containing Saturated Ring Structuresmentioning

confidence: 99%

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

Smyth¹,

Brooks

2004

Electrophoresis

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“…Nicotine related alkaloids belong to the group of very polar basic molecules, which in neutral and acidic solutions exist predominantly in the cationic form [4]. For this reason, HPLC separation of nicotine alkaloids can be carried out by ion pair [12], or ion chromatography [16], reversed-phase (RP) chromatography being the most common separation mode [11,[13][14][15]17].…”

Section: Introductionmentioning

confidence: 99%

“…The most common methods include thin layer chromatography [3], capillary electrophoresis [4][5][6], gas chromatography [7][8][9][10], and high performance liquid chromatography (HPLC) [11][12][13][14][15][16][17]. Each of these methods has its advantages.…”

Section: Introductionmentioning

confidence: 99%

“…Each of these methods has its advantages. However, HPLC with its various separation modes can have the capability of being more specific and sensitive, especially if interfaced with tandem mass spectrometry (MS/MS) [13][14][15][16][17]. Nicotine related alkaloids belong to the group of very polar basic molecules, which in neutral and acidic solutions exist predominantly in the cationic form [4].…”

Section: Introductionmentioning

confidence: 99%

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Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Taujenis¹,

Olšauskaitė²,

Padarauskas³

2015

Acta Chromatographica

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Summary.A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 µg/g for cotinine, 20 µg/g for anabasine and nornicotine, and 30 µg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

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“…To summarise, these methods include radioimmunoassay (6), high-performance liquid chromatography (7,8), as well as gas chromatography-mass spectrometry (GC-MS) (9)(10)(11)(12)(13)(14).…”

Section: Discussionmentioning

confidence: 99%

GC–MS determined cotinine in an epidemiological study on smoking status at delivery

Chazeron¹,

Daval²,

Ughetto³

et al. 2008

Pulmonary Pharmacology & Therapeutics

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A c c e p t e d m a n u s c r i p t AbstractThe objective of this study was to measure plasma cotinine levels in pregnant women and their newborns using a gas chromatography-mass spectrometry (GC-MS) method in an epidemiological delivered population with a wide range of tobacco intakes. Nearly one thousand pregnant women from regional maternity wards (n = 1007) were selected for the study. Each patient kept a tobacco diary and underwent a blood test to assess cotinine levels at the same time that newborns' cordonal plasma was taken. These values were then crosschecked.Cotinine was estimated using a selected-ion monitoring mode with a 1.5 ng/ml quantification limit. Cotinine levels in mothers and newborns were highly correlated, whatever the mother's smoking status with a calculated cut-off for cotinine levels in active smokers of 21.5 ng/ml. Finally, cotinine determined through this GC-MS method offered a sensitive and accurate measure of tobacco exposition of pregnant women and their babies.

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Determination of nicotine and cotinine in rat plasma by liquid chromatography–tandem mass spectrometry

Cited by 41 publications

References 7 publications

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

A critical evaluation of high performance liquid chromatography‐electrospray ionisation‐mass spectrometry and capillary electrophoresis‐ electrospray‐mass spectrometry for the detection and determination of small molecules of significance in clinical and forensic science

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

GC–MS determined cotinine in an epidemiological study on smoking status at delivery

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