Determination of Nicotine Extracted from Eggplant and Green Pepper by HPLC

Abstract

Nicotine was separated from eggplant and green pepper seeds (Solanaceous) by High Performance Liquid Chromatography (HPLC).The concentration of nicotine in the eggplant extract (0.871-0.877 μg/ml) was determined by injecting standard material with 0.5 and 1.5 μg/ml, while the concentrations of nicotine in green pepper extract (0.613-0.618 μg/ml) was determined when the standard material was injected with 0.5 and 1.5 μg/ml. The qualitative chemical data was calculated from derivations of the standard material. Nicotine concentration was measured qualitatively in both extracts through the calibration curve and method of the standard addition. This technique has high accuracy and compatibility, bringing the proportion of relative recovery percentage of nicotine extracted from eggplant seeds to 95.880-103.104% and relative error of -4.119-3.104%. Relative recovery percentage of nicotine extracted from green pepper seeds was 97.284 -103.518 % and relative error was -2.716-3.518%.

Development and Validation of a Simple and Sensitive Reverse-Phase High Performance Liquid Chromatographic Method for the Determination of Ibuprofen in Pharmaceutical Suspensions

“…The results were represented as a percentage of recoveries, and the percent recovery mean was determined, where the ICH Q1(R2) recommendations outlined that the accuracy percent results be equal the value %100±2 in order for the test to be considered acceptable 21 . The accuracy was also estimated by calculating percent relative error which should be not more 5% 22 .…”

Biotic and Abiotic Elicitors Induce Biosynthesis and Accumulation of Some Withanolide Compounds in the Callus Culture of Withania somnifera L.

“…The gradient program was achieved for chromatographic separation with a linear gradient B% (from 0-100% in 15min.). The concentrations of withanolide compounds in the samples were estimated according to (Mohammed, 2019) as formula below:…”

Qualitative and Quantitative Determination of Dapagliflozin Propanediol Monohydrate and Its Related Substances and Degradation Products Using LC-MS and Preparative Chromatography Methods

“…The aim of this work is to develop both validated analytical RP-HPLC/MS and preparative chromatographic methods for the qualitative and quantitative determination of DPG-related substances in the active pharmaceutical ingredient API and pharmaceutical dosage forms. The developed method was successfully validated as per ICH Q2 (R1) [23][24][25][26] .…”