Determination of nimodipine in human plasma by a sensitive and selective liquid chromatography–tandem mass spectrometry method

Qiu, Feng; Chen, Xiaoyan; Li, Xiaoyan; Zhong, Dafang

doi:10.1016/j.jchromb.2003.12.001

Cited by 31 publications

(32 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results demonstrated, however, that the mean half-life of nimodipine for the two formulations tested was 27.83 h (test formulation) and 32.78 h (reference formulation). Such a discrepancy could be due to the greater sensitivity of our method, with a limit of quantification of 100 pg/mL, while values found in previous studies were in the range 0.24 to 5 ng/mL (Krol et al, 1984;Jackobsen et al, 1986;Rosseel et al, 1990;Qian, Gallo, 1992;Fischer et al, 1993;Yan et al, 1993;Mück, 1995;Aymard et al, 1998;Gualano et al, 1999;López et al, 2000;Blardi et al, 2002;Qiu et al, 2004;Zhonggui et al, 2004;Nirogi et al, 2006). This improved sensitivity enabled the determination of plasma drug concentrations up to 48 h after drug administration, which was not possible in other studies that allowed the measurement of plasma drug levels only up to 10 h after drug administration.…”

Section: Pharmacokinetic Studymentioning

confidence: 59%

“…The present LC-MS-MS method offered an LLOQ of 100 pg/mL with a mean accuracy of 7.0% and a mean precision of 103.0% (n=5), which is more sensitive than or comparable to reported methods (Krol et al, 1984;Jackobsen et al, 1986;Rosseel Bogaert, Huyghens, 1990;Qian, Gallo, 1992;Aymard et al, 1998;López et al, 2000;Blardi et al, 2002;Mück, 1995;Qiu et al, 2004;Nirogi et al, 2006;Qin et al, 2008). Data for LLOQ are presented in Table II.…”

Section: Linearity and Lower Limit Of Quantificationmentioning

confidence: 72%

“…The presented method is not entirely different from those described earlier (Gualano et al, 1999;Qiu et al, 2004;Nirogi et al, 2006;Qin et al, 2008) but combines lower plasma volume (300 mL), short retention time (2.28 min for nimodipine and 2.58 min for dibucaine), a simple extraction procedure, sensitivity (100 pg/mL), accuracy, precision and linearity.…”

Section: Linearity and Lower Limit Of Quantificationmentioning

confidence: 94%

“…(Yan, Ding, Liu, 1993;Lian-Quing, Heng-Shan, Gang, 1993;Dollery, 1999;Blardi et al, 2002;Qiu et al, 2004;Zhonggui et al, 2004;Hernandez-Hernandez et al, 2002). Previous studies showed these data, but sampling was concluded between 5 and 10h after dosing, thereby possibly missing a longer elimination phase.…”

Section: Pharmacokinetic Studymentioning

confidence: 99%

“…This method was used for the quantification of nimodipine enantiomers. Gualano et al (1999) and Qiu et al (2004) reported an LC-MS/MS method with an atmospheric pressure chemical ionization (APCI) source in selected reaction monitoring mode for the determination of nimodipine in human plasma with an LLOQ of 0.25 and 0.24 ng/mL, respectively. Nirogi et al (2006) developed an LC-MS-MS method to determine nimodipine in human plasma which had an LLOQ of 200 pg/mL using 0.5mL plasma.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application

Nascimento

Moraes

Bezerra

et al. 2010

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

To develop and validate a rapid, specific and highly sensitive method to quantify nimodipine in human plasma using dibucaine as the internal standard (IS). The analyte and IS were extracted from plasma samples by liquid-liquid extraction using hexane-ethyl acetate (1:1 v/v). The chromatographic separation was performed on a Varian ® Polaris C18 analytical column (3 mm, 50 x 2.0 mm) and pre-column Securityguard TM C18 (4.0 x 3.0 mm) with a mobile phase of Acetonitrile-Ammonium acetate 0.02 ml/L (80:20v/v). The method had a chromatographic run time of 4.5 min and linear calibration curve over the range of 0.1-40 ng/mL (r > 0.9938). The limit of quantification was 100 pg/mL. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. This validated method was successfully applied in determining the pharmacokinetic profile of nimodipine tablets of 30 mg administered to 24 healthy volunteers. The proposed method of analysis provided a sensitive and specific assay for nimodipine determination in human plasma. The time for the determination of one plasma sample was 4.5 min. This method is suitable for the analysis of nimodipine in human plasma samples collected for pharmacokinetic, bioavailability or bioequivalence studies in humans.Uniterms: Nimodipine/determination in plasma. Nimodipine/pharmacokinetic profile. Nimodipine/ quantitative analysis. Pharmacokinetics/method validation.Um método rápido, específico e sensível para quantificar nimodipino em plasma humano usando dibucaína como padrão interno (IS) é descrito. O analito e o IS foram extraídos das amostras de plasma por extração líquido-líquido usando hexano-acetato de etila (1:1 v/v). A separação cromatográfica foi realizada utilizando-se uma coluna analítica C18 Varian ® Polaris (3 mm, 50 x 2,0 mm) e uma pré-coluna Securityguard TM C18 (4,0 x 3,0 mm) e acetonitrila-acetato de amônia 0,02 mol/L (80:20 v/v) como fase móvel. O método apresentou tempo total de corrida de 4,5 min e curva de calibração linear com concentrações entre 0,1-40 ng/mL (r > 0,9938). O limite de quantificação foi de 100 pg/mL. Os valores de precisão e exatidão foram obtidos por meio da análise das amostras de controle de qualidade. A análise de uma única amostra de plasma foi realizada em 4,5 minutos. A metodologia validada foi aplicada na determinação do perfil farmacocinético do nimodipino, comprimido de 30 mg administrado em 24 voluntários saudáveis. O método para quantificar nimodipino em plasma é adequado para aplicação em estudos farmacocinéticos, biodisponibilidade e bioequivalência em humanos.

show abstract

Section: Pharmacokinetic Studymentioning

confidence: 59%

Section: Linearity and Lower Limit Of Quantificationmentioning

confidence: 72%

Section: Linearity and Lower Limit Of Quantificationmentioning

confidence: 94%

Section: Pharmacokinetic Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application

Nascimento

Moraes

Bezerra

et al. 2010

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

Fragmentation of Drugs and Pesticides

2017

Interpretation of MS‐MS Mass Spectra of Drugs and Pesticides

View full text Add to dashboard Cite

Current literature in mass spectrometry

2004

J. Mass Spectrom.

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 9th. June 2004)

show abstract

Determination of nimodipine in human plasma by a sensitive and selective liquid chromatography–tandem mass spectrometry method

Cited by 31 publications

References 17 publications

Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application

Determination of nimodipine in plasma by HPLC-MS/MS and pharmacokinetic application

Fragmentation of Drugs and Pesticides

Current literature in mass spectrometry

Contact Info

Product

Resources

About