Determination of Nitrite and Nitrate by Vanadium(III) Reduction with Chemiluminescence Detection

Hendrix, Steven A.; Braman, Robert S.

doi:10.1006/meth.1995.1013

Cited by 15 publications

(4 citation statements)

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“…The concentrations of NO 2 − and NO 3 − in the medium were determined with a redox chemiluminescence NO analyser (FES‐450, Scholar Tec, Osaka, Japan) essentially as described previously (Kitade et al , 1996). Briefly, 200 μl of the medium was mixed with 2 ml of saturated ascorbic acid solution in a sealed tube for 10 to 15 s at room temperature to reduce NO 2 − to NO or with 2 ml of 0.1 M vanadium trichloride in 1 N HCl solution in a sealed tube for 60 s at 70°C to reduce NO 2 − and NO 3 − to NO (Hendrix & Braman, 1995). Measurement of NO was based on the observation of chemiluminescence (660–900 nm) produced by the reaction of NO with ozone.…”

Section: Methodsmentioning

confidence: 99%

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂

Sakai

Minami

Hara

et al. 1998

British J Pharmacology

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1 We recently demonstrated that intrathecal administration of prostaglandin E 2 (PGE 2 ) and PGF 2a induced allodynia through a pathway that includes the glutamate receptor and nitric oxide (NO)-generating systems from pharmacological studies. In order to clarify the involvement of NO in prostaglandin-induced allodynia, we measured NO released from rat spinal cord slices by a chemiluminescence method. 2 PGE 2 stimulated NO release from both dorsal and ventral regions all along the spinal cord. PGE 2 stimulated the release within 10 min and increased it in a time-dependent manner. 3 The PGE 2 -induced NO release was observed at 100 nM ± 10 mM. PGF 2a stimulated the release at concentrations higher than 1 mM, but PGD 2 (up to 10 mM) did not enhance it. 4 17-Phenyl-o-trinor PGE 2 (EP 1 4EP 3 ) and sulprostone (EP 1 5EP 3 ) were as potent as PGE 2 , but PGE 1 was less potent, in stimulating NO release. While M&B 28767 (EP 3 ) did not enhance the release, butaprost (EP 2 ) stimulated it at 1 mM. The PGE 2 -evoked release was blocked by ONO-NT-012, a bifunctional EP 1 antagonist/EP 3 agonist. 5 The PGE 2 -evoked release was Ca 2+ -dependent and blocked by MK-801 (NMDA receptor antagonist) and L-NAME (NO synthase inhibitor). The release was also inhibited by PGD 2 and dibutyryl-cyclic AMP. 6 The present study demonstrated that PGE 2 stimulates NO release in the rat spinal cord by activation of NMDA receptors through the EP 1 receptor, and supports our previous ®ndings that the NOgenerating system is involved in the PGE 2 -induced allodynia.

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Section: Methodsmentioning

confidence: 99%

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂

Sakai

Minami

Hara

et al. 1998

British J Pharmacology

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“…The amount of nitrite contained in saliva sample was found to be 52 mM. Taking into account prior dilution, the original content in the saliva sample was calculated to be 104 mM, which is close to other values reported in literature [18,23,24]. In order to confirm that there was no interference from the matrix, the centrifuged and diluted saliva samples were spiked with a known quantity of nitrite to evaluate the recovery of the method.…”

mentioning

confidence: 96%

Lignosulfonate‐Modified Electrode for Electrocatalytic Reduction of Acidic Nitrite

Milczarek

2008

Electroanalysis

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Sodium lignosulfonate (LS) undergoes oxidative electropolymerization on a glassy carbon (GC) electrode from sulfuric acid solution to form a chemically modified electrode exhibiting anionic character and redox activity. Cyclic voltammetry reveals the existence of two redox systems at E8' values of þ 0.29 and þ 0.53 V, respectively. Peak currents are proportional to the scan rate as expected for surface confined systems. The GC j poly-LS electrode shows electrocatalytic activity toward the reduction of acidic nitrite. When operating in a constant potential amperometric mode (at 0.0 V, vs. Ag/AgCl), a linear relationship between nitrite concentration and reduction current is observed over the range of 1 to 250 mM. The detection limit reaches 0.3 mM (S/N ¼ 3). The electrode may be practically applied for nitrite determination in human saliva.

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“…For instance, V(III) reduction plus chemiluminescence had a response time of 2 to ca. 8 min [39] comparing to less than 4 s for the method proposed here.…”

Section: Amperometry Of Chemically Generated No On a Polymercoated Elmentioning

confidence: 62%

Selective and sensitive detection of nitrite based on NO sensing on a polymer-coated rotating disc electrode

Milczarek

2007

Journal of Electroanalytical Chemistry

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Determination of Nitrite and Nitrate by Vanadium(III) Reduction with Chemiluminescence Detection

Cited by 15 publications

References 0 publications

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂

Lignosulfonate‐Modified Electrode for Electrocatalytic Reduction of Acidic Nitrite

Selective and sensitive detection of nitrite based on NO sensing on a polymer-coated rotating disc electrode

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Determination of Nitrite and Nitrate by Vanadium(III) Reduction with Chemiluminescence Detection

Cited by 15 publications

References 0 publications

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E2

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E2

Lignosulfonate‐Modified Electrode for Electrocatalytic Reduction of Acidic Nitrite

Selective and sensitive detection of nitrite based on NO sensing on a polymer-coated rotating disc electrode

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Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂

Stimulation of nitric oxide release from rat spinal cord by prostaglandin E₂