Determination of nitrite/nitrate in human biological material by the simple Griess reaction

Guevara, Ibeth; Iwanejko, J; Dembińska-Kieć, A.; Pankiewicz, Joanna; Wanat, Alicja; Polus, Anna; Goła̧bek, I.; Bartuś, Stanisław; Malczewska-Malec, Małgorzata; Szczudlik, Andrzej

doi:10.1016/s0009-8981(98)00060-6

Cited by 561 publications

(337 citation statements)

References 36 publications

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“…The animals were housed in an air conditioned room and were kept in standard laboratory conditions under normal light and dark cycle (12 …”

Section: Experimental Animalsmentioning

confidence: 99%

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Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti–apoptotic study

Upaganlawar

Vaibhav

Balaraman

2012

Asian Pacific Journal of Tropical Biomedicine

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“…The animals were housed in an air conditioned room and were kept in standard laboratory conditions under normal light and dark cycle (12 …”

Section: Experimental Animalsmentioning

confidence: 99%

“…Tissue nitrite content was estimated colorimetrically with the Griess reagent [12] in protein free supernatant of heart homogenate. Tissue vitamin E level was estimated as given by Desai [13] and serum protein levels was determined using reagent kit (Span Diagnostic Ltd, Gujarat, India).…”

Section: Biochemical Estimationsmentioning

confidence: 99%

Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti–apoptotic study

Upaganlawar

Vaibhav

Balaraman

2012

Asian Pacific Journal of Tropical Biomedicine

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“…Samples were deproteinized with 900 l of methanol/ether (3:1) mix for 1 h to avoid Griess reagent precipitation. The samples were next centrifuged at 16.1 ϫ g for 20 min, the supernatant collected, and the total amount of nitrite assayed with Griess reagent (36). A standard curve was generated as described above.…”

Section: No Measurement In Air Pouch Exudatesmentioning

confidence: 99%

The Arthritis Severity Quantitative Trait Loci Cia4 and Cia6 Regulate Neutrophil Migration into Inflammatory Sites and Levels of TNF-α and Nitric Oxide

Laragione

Yarlett

Mello

et al. 2007

The Journal of Immunology

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Neutrophils are required for the development of arthritis, and their migration into the synovial tissue coincides with the onset of clinical disease. Synovial neutrophil numbers also correlate with rheumatoid arthritis disease activity and severity. We hypothesized that certain arthritis severity genes regulate disease via the regulation of neutrophil migration into the joint. This hypothesis was tested in the synovial-like air pouch model injected with carrageenan using arthritis-susceptible DA and arthritis-resistant F344 rats. DA had nearly 3-fold higher numbers of exudate neutrophils compared with F344 (p < 0.001). Five DA.F344(QTL) strains congenic for severity loci and protected from autoimmune arthritis were studied. Only DA.F344(Cia4) (chromosome 7) and DA.F344(Cia6) (chromosome 8) congenics had significantly lower exudate neutrophil counts compared with DA. TNF-α levels were 2.5-fold higher in DA exudates as compared with F344 exudates, and that difference was accounted for by the Cia4 locus. Exudate levels of NO, a known inhibitor of neutrophil chemotaxis, were higher in F344, compared with DA, and that difference was accounted for by Cia6. This is the first time that non-MHC autoimmune arthritis loci are found to regulate three central components of the innate immune response implicated in disease pathogenesis, namely neutrophil migration into an inflammatory site, as well as exudate levels of TNF-α and NO. These observations underscore the importance of identifying the Cia4 and Cia6 genes, and suggest that they should generate useful novel targets for development of new therapies.

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“…Nitrite concentration was measured with the spectrophotometric Griess reaction at 548 nm. For nitrate measurement, this compound was first reduced to nitrites in presence of nitrate reductase [16].…”

Section: Measurement Of Erythrocyte Nitrite and Nitrate Concentrationsmentioning

confidence: 99%

Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology

Ahamd¹,

F.²,

Saldanha³

et al. 2017

Clin Med Invest

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Erythrocyte deformability is a biorheological influent factor on blood viscosity, cellular oxygenation and a biomarker of acute and chronic inflammation. An inverse relationship between erythrocyte ability to reversibly change its shape with nitric oxide (NO) efflux from erythrocytes was reported in patients with hypercholesterolemia, hypertension and renal transplant. Erythrocyte NO efflux is negatively associated with carotid intima-media thickness and an independent predictor of this structural anomaly. High concentrations of oxidized LDL when in presence of healthy human erythrocytes increase its ability to scavenge NO.The aim of this work was to evaluate in vitro, the effects on erythrocyte nitric oxide (NO) metabolism and its deformability under presence of lipoproteins LDL / HDL.Aliquots of healthy human blood samples were enriched, or not (control), with synthetic LDL/HDL solutions ([LDL/HDL] 1 =18.44/0.776 mg/dL and [LDL/ HDL] 2 =36.88/1.552 mg/dL). NO efflux from erythrocyte and its content on nitrite, nitrate, S-nitrosoglutathione (GSNO), peroxynitrite and its deformability (EEI) were determined. So, from each blood sample three aliquots were made: the control and two different LDL/HDL concentrations.In the present study, were not observed variations on peroxynitrite levels inside erythrocytes, on the ability to scavenge NO and on EEI at all shear stress when compared to the values obtained in untreated samples. NO mobilization inside erythrocytes of the treated aliquots were showed by the significantly increased of nitrite, nitrate and S-nitrosoglutathione levels in relation to the control aliquots without significant differences between both treated aliquots.In this in vitro study with erythrocytes obtained from blood samples of healthy human we show that under LDL/HDL addition, maintenance of its membrane deformability, nitric oxide and the nitrogen redox level content. All these results evidence the ability of erythrocytes to answer LDL/HDL binding by increasing NO mobilization with higher nitrite, nitrate and GSNO concentrations. Better understanding of the signal transduction mechanism associated to the erythrocytes binding LDL/HDL receptors may bring potential targets in cases of diminished erythrocyte deformability or impeachment delivery of nitric oxide to alleviate the compromised microcirculation.

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Determination of nitrite/nitrate in human biological material by the simple Griess reaction

Cited by 561 publications

References 36 publications

Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti–apoptotic study

Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti–apoptotic study

The Arthritis Severity Quantitative Trait Loci Cia4 and Cia6 Regulate Neutrophil Migration into Inflammatory Sites and Levels of TNF-α and Nitric Oxide

Lipoproteins effects on erythrocyte nitric oxide metabolism and biorheology

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