1954
DOI: 10.1002/jsfa.2740050803
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Determination of nitrogen in agricultural materials by the nessler reagent. II.—Micro‐determinations in Plant Tissue and in Soil Extracts

Abstract: The total nitrogen content of plant tissue may be determined by direct nesslerization of sulphuric acid-hydrogen peroxide digests with accuracy up t o 5%. Similar application to soil extracts involves somewhat greater errors.In a spectrophotometric study of the Nessler colour, a filter showing maximum transmission a t 420 mp absorbs the greatest proportion of the colour.In the sulphuric acid-hydrogen peroxide digestion of plant tissue, added ammonia is fully recovered but added nitrate suffers a loss of 24-47y… Show more

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Cited by 142 publications
(45 citation statements)
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“…This mixture was boiled at 350°C for 20 min, allowed to cool and 2 ml hydrogen peroxide added. The process was repeated 3 times until the samples became clear, after which a final heat treatment at 350°C for 30 min was applied (Yuen and Pollard 1954). Nitrogen was colorimetrically determined by indophenol blue (Searle 1984).…”
Section: Leaf Nitrogenmentioning
confidence: 99%
“…This mixture was boiled at 350°C for 20 min, allowed to cool and 2 ml hydrogen peroxide added. The process was repeated 3 times until the samples became clear, after which a final heat treatment at 350°C for 30 min was applied (Yuen and Pollard 1954). Nitrogen was colorimetrically determined by indophenol blue (Searle 1984).…”
Section: Leaf Nitrogenmentioning
confidence: 99%
“…loxophleba were used. Nitrogen was determined on a four-channel autoanalyser system, using modifications of the Technicon procedure (Anonymous, 1977), colorimetrically by the indophenol blue method after digestion with sulphuric acid and hydrogen peroxide (Yuen & Pollard, 1954). Phosphorus, K, Na, Ca, Mg, Cu, Fe, Mn and Zn were determined by inductively coupled plasma atomic emission spectroscopy on the same digest.…”
Section: Plant Materials and Soilsmentioning
confidence: 99%
“…The dried samples were ground and sent to CSBP laboratories for analyses. Subsamples of the dried ground herbage were dissolved in a nitric acid and perchloric acid mixture (Yuen and Pollard 1954) and concentrations of elements in the digest were measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES) (Zarcinas et al 1987). The concentration of the following elements were measured in dried herbage and reported: P, K, N, S, Cu, Zn, Mn, iron (Fe), calcium (Ca), magnesium (Mg) and boron (B).…”
Section: Soil Testingmentioning
confidence: 99%