Determination of nitroimidazole residues in poultry tissues, serum and eggs by high‐performance liquid chromatography

Abstract

A simple high-performance liquid chromatographic (HPLC) method was developed for determination of dimetridazole and metronidazole residues in poultry muscle, liver, serum and eggs. The drug residues were extracted from tissues and egg samples with acetonitrile followed by solid-phase extraction clean-up and HPLC analysis with photodiode array detector. Serum samples were treated with trichloroacetic acid, centrifuged and analysed without further clean-up. The detection limits were 2 ng/g and 5 ng/mL of analysed tissue (egg) and serum samples, respectively. Average recoveries for spike levels of 10 and 20 ng/g ranged from 82 to 94%, and coefficients of variation were below 10%.

Simultaneous determination of seven nitroimidazole residues in meat by using HPLC-UV detection with solid-phase extraction

“…The LOD value for all nitroimidazoles and metabolites was 0.2 g/kg, and the LOQ value was 0.7 g/kg. The obtained detection limit for this method is very lower than that for the methods reported in literature [19][20][21][22][23][24][25]27,28,30]. The proposed method can permit the detection of the seven nitroimidazoles in chiken and porcine tissue samples at level of ≥0.7 g/kg.…”

“…Gaugain and Abjean reported a TLC method for the detection of RNZ, DMZ and DMOHZ in animal muscle with the detection limits of 2-5 g/kg [20]. Several HPLC methods were described for the detection and identification of DMZ residues in muscle tissue with a confirmation limit of 5 g/kg [21], as well as DMZ, MNZ in poultry tissue, serum and eggs [22]. A solid-phase extraction-HPLC method was reported for the determination of MNZ in vaginal tissue with a detection limit of 100 g/kg [24].…”

“…Several analytical methods have been reported for the determination of nitroimidazole either in pure, dosage or in biological fluids and animal tissues, including spectrophotometry [11,12], electrophoresis [13], gas chromatography (GC) [14][15][16], GC-mass spectrometry (MS) [17,18], thin-layer chromatographic (TLC) [19,20], HPLC [21][22][23][24][25][26], and liquid chromatography (LC)-MS [10,[27][28][29][30]. Among them, chromatography has been utilized extensively for the determination of nitroimidazoles.…”

Metronidazole pharmacokinetics during rapid growth in turkeys – relation to changes in haemodynamics and drug metabolism

“…Plasma concentrations of MTZ and MTZ‐OH were measured by means of a modified reverse‐phase high‐performance liquid chromatography (HPLC) method reported previously by Semeniuk et al . (). Waters Alliance HPLC system (Waters, Milford, MA, USA) equipped with a 2996 PDA detector and a PLRP‐S 5 μm, 100 Å, 150/2.1 mm column (Agilent Technologies, Santa Clara, CA, USA) attached to an appropriate guard column were used to separate and quantify MTZ and MTZ‐OH.…”

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

“…Quite a few papers have been published describing the determination of dimetridazole and its main metabolite 2-hydroxydimetridazole in feed, tissues and eggs. In these papers, methods based on techniques such as differential pulse polarography [13], LC [14][15][16][17][18][19][20][21][22], GC [23][24][25][26][27][28][29] and LC-MS [4,5,[30][31][32][33][34][35][36] were described. Out of all the papers which have been published on the determination of halofuginone in different matrices, only few of them used LC-MS [4,5,[37][38][39].…”