Determination of Nitroimidazoles in Biological Fluids by Differential Pulse Polarography

Brooks, Marvin A.; D'Arconte, L.; Silva, J.A.F. De

doi:10.1002/jps.2600650125

Cited by 16 publications

(3 citation statements)

References 8 publications

(8 reference statements)

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“…92 Sensitivity was comparable with detection limits in the region of 0.05 pg/ml (10~7 At). The reduction of a nitro group has been used in several drug analyses, both for systems containing a nitro group, such as the nitroimidizoles, 93 and for nitro-containing derivatives of electroinactive drugs. Phenobarbital in blood was determined by differential pulse polarography after extraction and nitration.…”

Section: Nadh + (Dcpip) Ox D I°p H O R a S L Nad* + (Dcplp) Redmentioning

confidence: 99%

Bioelectrochemistry: An Examination of Some Examples

McCreery¹,

Wilson²

1978

C R C Critical Reviews in Analytical Chemistry

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Section: Nadh + (Dcpip) Ox D I°p H O R a S L Nad* + (Dcplp) Redmentioning

confidence: 99%

Bioelectrochemistry: An Examination of Some Examples

McCreery¹,

Wilson²

1978

C R C Critical Reviews in Analytical Chemistry

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“…Voltammetric methods have been applied to the study of the mechanism of nitroimidazoles as antimicrobial agents 20 , and its determination in pharmaceutical [21][22][23][24][25] and clinical [25][26][27] matrces. The mercury electrode was the most used, but good results were obtained using solid electrodes when studying nitrobenzene [28][29][30] and metronidazole [31][32][33] .…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

La-Scalea¹,

Serrano²,

Gutz³

1999

J. Braz. Chem. Soc.

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Metronidazol é o fármaco mais importante do grupo de 5-nitroimidazoles, possuindo toxicidade contra microrganismos anaeróbicos, sendo a molécula de DNA o principal alvo de sua ação biológica. O mecanismo de ação do metronidazol depende do processo de redução do grupo nitro e, em meio ácido, a redução é pH-dependente envolvendo quatro elétrons na completa redução ao derivado hidroxilamínico. Em meio aprótico a redução do metronidazole acontece em duas etapas: a primeira envolvendo um elétron para formar o radical nitro e a segunda etapa envolve mais três elétrons até a formação da hidroxilamina. Neste artigo o mecanismo de redução do metronidazol foi estudado utilizando as técnicas voltamétricas: polarografia d.c. e pulso diferencial, voltametria cíclica usando o eletrodo gota de mercúrio como eletrodo de trabalho. Metronidazole is the most important drug of the group of 5-nitroimidazoles and possesses toxicity to anaerobic microorganisms DNA being the main target for their biological action. The mechanism of biological action of metronidazole is dependent upon the nitro group reduction process. The reduction of metronidazole is pH dependent in acid medium and four electrons are involved in the complete reduction to the hydroxylamine derivative. In aprotic medium the reduction of the metronidazole occurs in two steps, the first involving one electron to form the nitro radical and the second step involving three more electrons until the formation of the hydroxylamine derivative. In this paper the mechanism of reduction of metronidazole was studied by using the voltammetric techniques: d.c. polarography, differential pulse polarography and cyclic voltammetry using the mercury drop as the working electrode.

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“…Die differentielle Pulspolarographie") wurde in einer modifizierten Form auch zur Bestimmung in biologischen Flussigkeiten eingesetzt 19). Die verschiedenen Techniken ,(Oszillo-, DC-, SW-und Puls-Polarographie) r Abb.…”

Section: Voltammetrieunclassified

Analytische Methoden für die Stabilitätsprüfung von Metronidazol‐Infusionslösungen

Ebel

Ledermann

Mümmler

1990

Archiv der Pharmazie

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UV-spectroscopy, thin layer- and high pressure liquid-chromatography as well as voltammetry (polarography) are suggested and compared as methods for use in stability testing of solutions of metronidazol. If some conditions are fulfilled UV-spectroscopy is the method of highest precision. TLC/HPTLC has advantages compared to HPLC, for more polar degradation products are visible at the starting point, whereas in HPLC these substances may be irreversibly adsorbed on the columne. Combination of HPLC and voltammetric (polarographic) techniques may lead to the optimal solution of this analytical problem with respect to best accuracy and precision (reproducibility) as compared in this paper.

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Determination of Nitroimidazoles in Biological Fluids by Differential Pulse Polarography

Cited by 16 publications

References 8 publications

Bioelectrochemistry: An Examination of Some Examples

Bioelectrochemistry: An Examination of Some Examples

Voltammetric Behaviour of Metronidazole at Mercury Electrodes

Analytische Methoden für die Stabilitätsprüfung von Metronidazol‐Infusionslösungen

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