Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects the cloven-hooved animals causing serious economic losses [1]. FMD virus belongs to the family Picornaviridae. It has an approximately 8 kB RNA genome encoding a single polyprotein that is cleaved into 12 different proteins by the help of viral proteases. Among these proteins, there are structural virus capsid proteins (SPs); VP1-4, and the non-structural proteins (NSPs); L, 2A, 2B, 2C, 3A, 3B, 3C, and 3D. Since the NSPs are clarified during the vaccine production, vaccinated animals elicit antibodies only against SPs, but FMD infected animals elicit antibodies against both SPs and NSPs [1-3]. Developed from this principle, Differentiating Infected from Vaccinated Animals (DIVA) tests are differentiated the vaccinated animal from the infected one. These tests are used in the serosurveilance activities of endemic countries, and have been