Determination of nonsteroidal anti‐inflammatory drugs in human specimens by capillary zone electrophoresis and micellar electrokinetic chromatography

Makino, Kazutaka; Itoh, Yoshinori; Teshima, Daisuke; Oishi, Ryozo

doi:10.1002/elps.200305870

Cited by 38 publications

(14 citation statements)

References 42 publications

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“…CE offers a variety of methods, such as CZE, MEKC, NACE, and separation of chiral compounds, which have been employed for the measurement of these compounds. This is shown by the use of CE in the simple and rapid monitoring of drugs in biological samples [111], serum drug monitoring [112], screening of biological specimens for drugs of forensic interest [113], analysis of illicit drugs in urine [114], determination of nonsteroidal antiinflammatory drugs in human specimens [115], analyzing protein-protein interactions in target-directed drug discovery [116], in pharmacokinetics [117], and antibiotic analysis [118]. In addition, a number of capillary-based serum drug assays have been described, including those for butabiltal [119], albandazole, and albendazole sulfoxide [120], and CE-based immunoassays for digoxin and gentamicinin [121].…”

Section: Drugsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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Over the past 15 years, CE as an analytical tool has shown great promise in replacing many conventional clinical laboratory methods, such as electrophoresis and HPLC. CE's appeal was that it was fast, used very small amounts of sample and reagents, was extremely versatile, and was able to separate large and small analytes, whether neutral or charged. Because of this versatility, numerous methods have been developed for analytes that are of clinical interest. Other than molecular diagnostic and forensic laboratories CE has not been able to make a major impact in the United States. In contrast, in Europe and Japan an increasing number of clinical laboratories are using CE. Now that automated multicapillary instruments are commercially available along with cost-effective test kits, CE may yet be accepted as an instrument that will be routinely used in the clinical laboratories. This review will focus on areas where CE has the potential to have the greatest impact on the clinical laboratory. These include analyses of proteins found in serum and urine, hemoglobin (A1c and variants), carbohydrate-deficient transferrin, forensic and therapeutic drug screening, and molecular diagnostics.

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Section: Drugsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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“…Combining with the above-mentioned differences in the distribution of charged and hydrophobic sites of the solutes in BDS modified capillary surface, separation efficiencies can be greatly increased by using BDS solution as running buffers. Coincidently, these effects of pure BDS solution on CE analysis nicely met one of the requirements for establishment of the MEKC separation mode [1][2][3].…”

Section: Effects Of Bds Solution Used As Supporting Electrolytementioning

confidence: 82%

Successful establishment of MEKC with electrochemiluminescence detection based on one functionalized ionic liquid

Fang

Wang

2009

Electrophoresis

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Herein, one water-soluble functionalized ionic liquid, 1-butyl-3-methylimidazolium dodecyl sulfate ([BMIm(+)][C(12)H(25)SO(-) (4)]), was designed and its superiorities either used as supporting electrolytes or as additives for successful establishment of MEKC with electrochemiluminescence (ECL) detection (MEKC-ECL) method were investigated. Compared with the common supporting electrolytes such as phosphate solution, 1-butyl-3-methylimidazolium dodecyl sulfate solution used as running buffers led to greatly enhanced ECL intensities and column efficiencies for negative targets, a little increase for neutral-charge ones while maintained nearly unchanged for positive ones due to the electrostatic forces between the large cation BMIm(+) and the solutes and the hydrophobic interactions resulting from the large anion C(12)H(25)SO(-) (4). Moreover, resolution efficiency between analytes was significantly improved. As the existence of ionic liquid moiety, BMIm(+), accelerated the electron transfer at the electrode surface, the poisoning effect of long chain C(12)H(25)SO(-) (4) on the electrode surface was eliminated and reproducible ECL intensities with relative standard derivation of 1.02% (n=6) were obtained. The proposed novel MEKC-ECL system was again validated by the baseline separated two similar amino acids of proline and hydroxyproline with lower detection limits of 0.5 and 0.8 microM (S/N=3), respectively.

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“…All of the selected NSAIDs are characterized by the presence of either phenol or amine groups, which can be electrochemically oxidized [51]. Consequently, capillary electrophoresis with electrochemical detection appears to be one of the most appropriate techniques for the direct analysis of this group of drugs.…”

Section: Resultsmentioning

confidence: 99%

“…Since these compounds are weak acids [51], they are more likely to be soluble in alkaline solutions. This property also allows the anionic forms of the NSAIDs to be separated on the basis of their electrophoretic mobility.…”

Section: Effect Of Run Buffer Phmentioning

confidence: 99%

Determination of Nonsteroidal Anti‐inflammatory Drugs in Serum by Microchip Capillary Electrophoresis with Electrochemical Detection

Ding

Garcı́a

2006

Electroanalysis

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A simple and rapid method has been developed for the analysis of four nonsteroidal anti-inflammatory drugs (NSAIDs) in serum using microchip capillary electrophoresis with pulsed amperometric detection. The selected NSAIDs (salicylic acid, acetaminophen, diflunisal, and diclofenac) are among the most commonly used drugs to treat fever, inflammation, and pain. Used above the therapeutic levels, these drugs can cause a wide variety of adverse effects and their fast analysis could have a significant impact in treatment and recovery of the patients. Several conditions, including separation potential, pH, and concentration of the electrolyte solution were studied to optimize the separation and detection. In this study, salicylic acid, acetaminophen, diflunisal, and diclofenac were separated in less than 2 minutes using a 5 mM borate buffer at pH 11.5 and a separation potential of þ 1200 V. Linear relationships were obtained between the concentration and peak current in the 0.5 -15.3 mg/mL range and detection limits around 0.26 mg/mL. After 30 consecutive injections, the stability of both the response and migration time of the analytes showed relative related deviations of less than 4.6% and 1.0%, respectively. The potential of this method was verified by spiking a bovine serum sample with the four NSAIDs and analyzing the recovery ratio.

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Determination of nonsteroidal anti‐inflammatory drugs in human specimens by capillary zone electrophoresis and micellar electrokinetic chromatography

Cited by 38 publications

References 42 publications

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis and the clinical laboratory

Successful establishment of MEKC with electrochemiluminescence detection based on one functionalized ionic liquid

Determination of Nonsteroidal Anti‐inflammatory Drugs in Serum by Microchip Capillary Electrophoresis with Electrochemical Detection

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