Determination of nucleic acids using phosphin 3R as a fluorescence probe

Zhu, Qing-Zhi; Yang, Huang‐Hao; Li, Donghui; Xu, Juntian

doi:10.1016/s0003-2670(99)00309-8

Cited by 37 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24 Generally, fluorescence probes including organic dyes such as ethidium bromide (EB), 25 Hoechst 33258, 26 phosphine 3R, 27 and metal ions were employed to investigate DNA. However, many of these fluorescent DNA probes are highly toxic and can be mutagenic.…”

Section: Fluorescence Spectroscopic Studiesmentioning

confidence: 99%

DNA Probe and Cleavage Studies Based on the Interaction between DNA and Yb, Er Complexes of 2‐[(Trifluoroaceto)aceto]thinophene‐piperidine Ligands

Yin

Huo

et al. 2009

Chin. J. Chem.

View full text Add to dashboard Cite

Two kinds of Ln complexes of [Ln(TTA) 4 ]•HP (Ln＝Yb or Er, TTA＝2-[(Trifluoroaceto)aceto]thinophene, HP ＝piperidine) have been synthesized and characterized, and their DNA-binding properties investigated using UV spectra, fluorescent spectra, viscometry and molecular modeling. The results show that they can intercalate into the double helices of DNA. More important thing is that their fluorescence intensity can be enhanced by DNA, thererfore, a sensitive fluorescence method for the determination of DNA may be developed. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. Interestingly, the complexes can cleave circular plasmid pBR322 DNA at pH＝7.2 and 37 . In addition, BDNPP ℃ [bis(2,4-dinitrophenyl)-phosphate] was chosen as a model compound to further study their cleavage mechanism of pBR322 DNA. From the first-order kinetics equation, it was proved indirectly that the mechanism may be a hydrolytic cleavage.

show abstract

Section: Fluorescence Spectroscopic Studiesmentioning

confidence: 99%

DNA Probe and Cleavage Studies Based on the Interaction between DNA and Yb, Er Complexes of 2‐[(Trifluoroaceto)aceto]thinophene‐piperidine Ligands

Yin

Huo

et al. 2009

Chin. J. Chem.

View full text Add to dashboard Cite

show abstract

“…[1] Such attention to studies on nucleic acids has been directed toward the fluorescence probes including organic dyes [2 -5] and metal complexes. They have an important function in life processes.…”

Section: Application Of Organic Nanoparticles As Fluorescence Probe Imentioning

confidence: 99%

Application of Organic Nanoparticles as Fluorescence Probe in the Determination of Nucleic Acids

et al. 2004

View full text Add to dashboard Cite

Organic fluorescent nanoparticles (NPs) have been successfully synthesized by reprecipitation method under ultrasonic irradiation. In comparison with single organic fluorophores, these NPs are brighter, photo-chemically stable, and water-soluble. At acidic medium, the fluorescence of NPs is 60 times strong as that of 1-pyrenemethylamine. They also have high room temperature fluorescence quantum yields (20%) and twice long fluorescence lifetime (0.2 msec) as that of single organic fluorophores. A fluorescence method has been developed for rapid determination of DNA with these organic NPs as a fluorescence probe, based on the fluorescence quenching of NPs in the presence of DNA. Maximum fluorescence quenching is produced at pH 2.0 with maximum excitation and emission wavelengths of 238 and 400 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range 0.4 -10.5 mg mL 21 for calf thymus DNA (ct-DNA) and 0.8 -20.0 mg mL 21 for fish sperm DNA (fs-DNA). The corresponding detection limit is 0.019 mg mL 21 for ct-DNA and 0.022 mg mL 21 for fs-DNA. The relative standard deviation of six replicate measurements is 1.3 -2.2%. The method is simple, rapid, and sensitive. The recovery and relative standard deviation are very satisfactory.

show abstract

“…Due to the well-known facts that the fluorescence intensity of DNA itself is very weak and heavy metal ions are not luminescent, it is hard to monitor the interactions of these metal ions with DNA by employing direct fluorescence emission method. Generally, fluorescent probes including organic dyes such as ethidium bromide [16], Hoechst 33258 [17] or Phosphin 3R [18] and rare-earth ions [19] are usually employed to investigate DNA. Based on the luminescence characteristics of rare-earth ions, especially Tb 3 þ and Eu 3 þ [20], whose resonance energy levels overlap with ultraviolet light energy [21], they are thus widely used as fluorescent probes to study DNA.…”

Section: Introductionmentioning

confidence: 99%

Methylene blue as a DNA probe for a comparative study of Cd2+, Pb2+ and Cr3+ ions binding to calf thymus DNA

Tong

Zhou

2011

Journal of Luminescence

View full text Add to dashboard Cite

Determination of nucleic acids using phosphin 3R as a fluorescence probe

Cited by 37 publications

References 20 publications

DNA Probe and Cleavage Studies Based on the Interaction between DNA and Yb, Er Complexes of 2‐[(Trifluoroaceto)aceto]thinophene‐piperidine Ligands

DNA Probe and Cleavage Studies Based on the Interaction between DNA and Yb, Er Complexes of 2‐[(Trifluoroaceto)aceto]thinophene‐piperidine Ligands

Application of Organic Nanoparticles as Fluorescence Probe in the Determination of Nucleic Acids

Methylene blue as a DNA probe for a comparative study of Cd2+, Pb2+ and Cr3+ ions binding to calf thymus DNA

Contact Info

Product

Resources

About