Determination of Ochratoxin A traces in foodstuffs: Comparison of an automated on-line two-dimensional high-performance liquid chromatography and off-line immunoaffinity-high-performance liquid chromatography system

Armutçu, Canan; Uzun, Lokman; Denizli, Adil

doi:10.1016/j.chroma.2018.07.057

Cited by 42 publications

(13 citation statements)

References 40 publications

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“…The recovery of both ochratoxins is improved in comparison with previous on-line SPE methods for the determination of OTA in wine, 92% in our method vs. 82% reported by Campone et al [32] and 76% documented by Bacaloni et al [30]. Although Armutcu et al [31] showed a good recovery of 105%, their method lacked our sensitivity, with a LOQ 0.5 µg L −1 . None of the method challenged the determination of the more polar ochratoxin, OTB, that is difficult to separate from the wine matrix.…”

Section: Discussioncontrasting

confidence: 53%

“…None of the method challenged the determination of the more polar ochratoxin, OTB, that is difficult to separate from the wine matrix. Moreover, our novel method is notably faster, with only 6 min including the on-line SPE step, which distinguishes it from the 22-35 min methods using online extractions in wine [30][31][32].…”

Section: Discussionmentioning

confidence: 97%

“…In the first step, the sample is purified in an extraction cartridge, and the chromatographic separation follows during the next step. Reports dealing with the online extraction and chromatography determination of ochratoxin A in beer [29,30] and wine [30][31][32] have recently been published, but none have focused on OTB.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography

Kholová

Lhotská

Uhrová

et al. 2020

Toxins

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According to the EU legislation, ochratoxin A contamination is controlled in wines. Tokaj wine is a special type of sweet wine produced from botrytized grapes infected by “noble rot” Botrytis cinerea. Although a high contamination was reported in sweet wines and noble rot grapes could be susceptible to coinfection with other fungi, including ochratoxigenic species, no screening of Tokaj wines for mycotoxin contamination has been carried out so far. Therefore, we developed an analytical method for the determination of ochratoxin A (OTA) and ochratoxin B (OTB) involving online SPE coupled to HPLC-FD using column switching to achieve the fast and sensitive control of mycotoxin contamination. The method was validated with recoveries ranging from 91.6% to 99.1% with an RSD less than 2%. The limits of quantification were 0.1 and 0.2 µg L−1 for OTA and OTB, respectively. The total analysis time of the online SPE-HPLC-FD method was a mere 6 min. This high throughput enables routine analysis. Finally, we carried out an extensive investigation of the ochratoxin contamination in 59 Slovak Tokaj wines of 1959–2017 vintage. Only a few positives were detected. The OTA content in most of the checked wines did not exceed the EU maximum tolerable limit of 2 µg L−1, indicating a good quality of winegrowing and storing.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 97%

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Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography

Kholová

Lhotská

Uhrová

et al. 2020

Toxins

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“…In the 25 ml analytical solvent, the hyperoside standard of 2.5 mg, or the quercitrin standard of 5.0 mg, or the quercetin standard of 2.0 mg, or the pseudohypericin standard of 1.0 mg, or the hypericin standard of 1.0 mg are dissolved to prepared stock solution, respectively. According to the guide of International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (Singh, 2015; Armutcu et al, 2018; Hsi et al, 2019), the signal-to-noise ratio (S/N) of 3 and 10 were defined as the detection limit (LOD), and the quantitative limit (LOQ), respectively. In order to detect the S/N, the stock solution of hyperoside, quercitrin, quercetin, pseudohypericin, and hypericin were diluted to different concentrations, respectively.…”

Section: Methodsmentioning

confidence: 99%

Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.

et al. 2019

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Hypericum perforatum L., also known as Saint John’s Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of H. perforatum on infectious bronchitis virus (IBV) were evaluated in vitro and in vivo for the first time. The results of in vitro experiments confirmed that the antiviral component of H. perforatum was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative messenger ribonucleic acid (mRNA) expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. HPE treatment at doses of 480–120 mg/kg for 5 days, reduced IBV induced injury in the trachea and kidney, moreover, reduced the mRNA expression level of IBV in the trachea and kidney in vivo. The mRNA expression levels of IL-6, tumor necrosis factor alpha (TNF-α), and nuclear factor kappa beta (NF-κB) significantly decreased, but melanoma differentiation-associated protein 5 (MDA5), mitochondrial antiviral signaling gene, interferon alpha (IFN-α), and interferon beta (IFN-β) mRNA levels significantly increased in vitro and in vivo. Our findings demonstrated that HPE had significant anti-IBV effects in vitro and in vivo, respectively. In addition, it is possible owing to up-regulate mRNA expression of type I interferon through the MDA5 signaling pathway and down-regulate mRNA expression of IL-6 and TNF-α via the NF-κB signaling pathway. Moreover, the mainly active compositions of HPE analyzed by high-performance liquid chromatography/electrospray ionization–mass spectroscopy (ESI-MS) are hyperoside, quercitrin, quercetin, pseudohypericin, and hypericin, and a combination of these compounds could mediate the antiviral activities. This might accelerate our understanding of the antiviral effect of H. perforatum and provide new insights into the development of effective therapeutic strategies.

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“…Early and accurate detection of food safety is therefore very important for preventing, controlling, and mitigating the impact of potential outbreaks. Many analytic methods, including chromatography methods such as gas chromatography (GC) [2], high performance liquid chromatography (HPLC) [3], gas chromatography-mass spectrometer (GC-MS) [4], and liquid chromatography-mass spectrometer (LC-MS) [5], and immunological detection, such as enzyme linked immunosorbent assay (ELISA) [6] and lateral flow immunoassay [7], have been employed for food safety detection. Although those traditional methods are relatively sensitive and specific, they are expensive, laborious, and time-consuming and require well-trained personnel [8,9], which make them incompatible for developing countries and areas are lacking equipped facilities and specialists.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensors for Food Safety

Zeng¹,

Peng²,

Wu³

et al. 2019

Nutrition in Health and Disease - Our Challenges Now and Forthcoming Time

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Food safety poses an increasing threat to human health worldwide. The development of analytical methods and techniques to ensure food safety is therefore of great importance. Electrochemical sensors provide unique opportunity to realize sensitive, accurate, rapid, and portable detection for food safety. They have the potential to overcome the restrictions and limitations of traditional methods. In this chapter, we review the progress of electrochemical sensors for the detection of food contaminants including heavy metals, illegal additives, pesticide residues, veterinary drug residues, biological toxins, and foodborne pathogen. Future perspectives and challenges are also discussed.

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Determination of Ochratoxin A traces in foodstuffs: Comparison of an automated on-line two-dimensional high-performance liquid chromatography and off-line immunoaffinity-high-performance liquid chromatography system

Cited by 42 publications

References 40 publications

Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography

Determination of Ochratoxin A and Ochratoxin B in Archived Tokaj Wines (Vintage 1959–2017) Using On-Line Solid Phase Extraction Coupled to Liquid Chromatography

Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.

Electrochemical Sensors for Food Safety

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