Determination of oligosaccharides and monosaccharides in Hakka rice wine by precolumn derivation high-performance liquid chromatography

Abstract

This article presents a precolumn derivatization procedure with 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent to detect oligosaccharides and monosaccharides in Hakka rice wine. The subsequent separation of the derivatized glucose-PMP also was performed using a mobile phase consisting of the molar ratio of acetonitrile to ammonium acetate buffer (0.1M) of 22:78 at a flow rate of 1.0 mL/min with the column temperature of 35°C, and the pH of ammonium acetate buffer at 5.5. The optimum derivation conditions were as follows: reaction temperature, 70°C; reaction time, 30 minutes; molar ratio of PMP to glucose, 10:1 (v/v); molar ratio of sodium hydroxide to glucose, 3:1 (v/v). The recovery rates were between 93.13% and 102.08% with relative standard deviation of 0.96-2.48%. The established method provides sufficient sensitivity with values of limit of detection of 0.09-0.26 mg/L and limit of quantification of 0.27-0.87 mg/L for determination of oligosaccharides and monosaccharides.

Study on the ability of partially hydrolyzed guar gum to modulate the gut microbiota and relieve constipation

“…PHGG was hydrolyzed using 2 mol/L trifluoroacetic acid (TFA) at 110°C for 4 hr. The sample derivatization and further HPLC analysis procedures applied in this study were determined according to method reported by Bai et al (). The derived samples were analyzed with an Agilent 1200 Infinity HPLC system equipped with an Agilent Eclipse XDB‐C18 column (5 µm, 250 mm × 4.6 mm i.d.).…”

Hindbrain estrogen receptor regulation of ventromedial hypothalamic glycogen metabolism and glucoregulatory transmitter expression in the hypoglycemic male rat

“…Supernatant aliquots (20 μL) were hydrolyzed by incubation with 0.5 mg/ml amyloglucosidase (10 μL) and 0.1M sodium acetate (10 μL) for 2 hr prior to heating to 100ºC (5 min), followed by cooling to room temperature. Supernatant glycogen concentrations were determined by reverse-phase HPLC in a Hitachi LaChrom Elite® System (Hitachi America, Ltd., Tarrytown, NY), by modification of published methods [Honda et al, 1989;Fuller et al, 2012;Bai et al, 2015]. Hydrolyzed and non-hydrolyzed sample aliquots were derivatized with 100 μL 0.5 M 1-phenyl-3-methyl-5-pyrazolone (PMP) reagent supplemented with 0.3 M NaOH.…”

Application of Liquid Chromatography in Food Analysis

“…Total and resistant starch was measured in several matrices including (e.g., silages) [318]. Bai and coworkers analyzed mono-and oligosaccharides from Hakka rice [319] as a measure of quality for sugars such as isomaltotriose, isomaltose, panose, maltose, and glucose. Finally, the determination of bacterial exopolysaccharides has also been reported [320,331].…”