Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue

Tero-Vescan, Amelia; Ősz, Bianca-Eugenia; Vari, Camil-Eugen; Imre, Silvia; Hancu, Gabriel

doi:10.1515/amma-2015-0093

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Similar results were previously observed by Tero‐Vescan, Osz, Vari, Imre, and Hancu (), in which the HFD of the balanced ω‐6‐to‐ω‐3 ratio decreased the AA‐to‐EPA ratio because of decreased macrophage infiltration and reduction in prostaglandin E 2 concentration, which is a product of EPA.…”

Section: Discussionsupporting

confidence: 89%

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Ghazala

Medney

Meleis

et al. 2020

Biomedical Chromatography

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Lipotoxicity is defined as deposition of excess fat associated with an inflammatory response. Metabolomic analysis of fatty acids (FAs) can be a marker of silent inflammation. ω3‐Enriched diet, celecoxib, and safranal may have a protective anti‐inflammatory role. In this work, total FAs extracted from red blood cells and arachidonic acid‐to‐eicosapentaenoic acid (AA‐to‐EPA) ratios were assessed using GC–MS assay in single‐ion monitoring mode. The study was conducted on 64 male rats divided into eight groups: I, controls; II, rats received high‐fat diet (HFD), III, rats received ω‐6‐enriched HFD; IV, rats received ω‐3‐enriched HFD; V, rats received celecoxib with HFD; VI, rats received safranal with HFD; VII and VIII, rats received celecoxib and safranal with ω‐3 HFD, respectively. GC–MS Gas chromatography Mass spectrometry was performed for analysis of fatty acid methyl ester. Enzyme‐linked immunosorbent assay was used to analyze serum interleukin‐6 (IL‐6) and transforming growth factor‐beta 1 (TGF‐β1) concentrations. A statistically significant decrease of AA‐to‐EPA ratio was observed in group VII when compared with the groups receiving HFDs. This group also showed the lowest serum IL‐6 level and highest TGF‐β1 level. In conclusion, ω3‐enriched diet along with drugs (e.g. celecoxib) and herbal medications (e.g. safranal) may have an anti‐inflammatory effect in lipotoxicity. GC–MS with single‐ion monitoring is valid for the analysis of FAs.

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Section: Discussionsupporting

confidence: 89%

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Ghazala

Medney

Meleis

et al. 2020

Biomedical Chromatography

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“…environmental, biological, cosmetic, and food samples (including supplements) have been proposed, with the majority of LC‐based methods utilizing different types of columns and detection systems . However, procedures based on GC with NPD or MS detection as well as CE were also proposed. Direct analysis of CAF in food products is often limited due to insufficient sensitivity and matrix interferences.…”

Section: Introductionmentioning

confidence: 99%

Comparison of GC–MS and MEKC methods for caffeine determination in preworkout supplements

et al. 2019

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In this study, GC–MS‐ and MEKC‐based methods for determination of caffeine (CAF) in preworkout supplements were developed and validated. The proposed protocols utilized minimal sample preparation (simple dilution and syringe filtration). The developed methods achieved satisfactory validation parameters, i.e. good linearity (R2 > 0.9988 and R2 > 0.9985 for GC–MS‐ and MEKC‐based method, respectively), satisfactory intra‐ and interaccuracy (within 92.6–100.7% for method utilizing GC–MS and 92.1–110.3% for protocol based on MEKC) and precision (CV < 15.9% and CV < 6.3% for GC–MS‐ and MEKC‐based method, respectively) and recovery (within 100.1–100.8% for method utilizing GC–MS and 101.5–106.2% for protocol based on MEKC). The LOD was 0.03 and 3 μg/mL for method utilizing GC–MS and MEKC, respectively. The CAF concentrations determined by GC–MS‐ and MEKC‐based methods were found to be in the range of 8.53–11.23 and 8.20–11.61 μg/mL, respectively. Taking into consideration information on the labels, the investigated supplements were found to contain from 110.0 to 167.3% of the declared CAF content, which confirmed the literature reports on incompatibility of the declared product compositions with real ones. Nevertheless, the consumption of examined supplements as recommended by producers did not lead to exceeding the CAF safe limit of 400 mg per day. Additionally, the MEKC‐based method allowed for detection and identification of vitamin B3 and B6 in all of the investigated supplement samples, which demonstrated that MEKC‐based protocols may be an appropriate assays for simultaneous determination of CAF and vitamins.

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Determination of Omega-3/Omega-6 Ratio in Swine Brain Homogenate as an Animal Model for Human Nervous Tissue

Cited by 2 publications

References 18 publications

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Comparison of GC–MS and MEKC methods for caffeine determination in preworkout supplements

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