Determination of operating parameters of fungal growth signals analyzed by laser speckle contrast imaging

Abstract

Our previous studies have shown that laser speckle imaging with sensitive subpixel correlation analysis is able to detect bacterial growth activity and the pattern of colony growth. In the current study, we demonstrate the potential of this method to analyze fungal growth. We compare the characteristics of the signals obtained from bacteria and fungi. The obtained results will help to improve the parameters of the speckle image acquisition system and the signal processing algorithms useful for microorganism (both eukaryotic and prokaryotic) growth analyses and speeding up and facilitating microbiological diagnostics.