Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa

Vasconcelos, André Belico de; Souza, Patrícia C.; Varago, F. C.; Lagares, Monique de Albuquerque; Santoro, Marcelo M.

doi:10.1590/s1516-89132009000500010

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…The semen was then diluted with Kenney extender (Kenney et al. , 1975) to obtain samples containing a sperm number of approximately 1 × 10 8 sperm ml −1 (Vasconcelos et al. , 2008).…”

Section: Methodsmentioning

confidence: 99%

“…After 2 h cooling to 5 °C, sperm motility, viability and membrane integrity of the semen samples were evaluated and each 1.5 ml semen sample was submitted to three treatments (i) Addition of 6 m m of glucose (Vasconcelos et al. , 2008) to the diluted semen with Kenney extender (sperm concentration of 1 × 10 8 sperm ml −1 ), (ii) Removal of seminal plasma through centrifugation (800 g , 10 min) until 10% of total semen volume and re‐suspension with 1 ml of Kenney extender and (iii) Centrifugation (800 g , 10 min) and homogenisation with its own seminal plasma.…”

Section: Methodsmentioning

confidence: 99%

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Metabolic evaluation of cooled equine spermatozoa

Vasconcelos¹,

Santana

Santos

et al. 2010

Andrologia

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Microscopy has been used in the routine evaluation of sperm metabolism. Nevertheless, it has limited capacity to preview male fertility. As calorimetry may be used to evaluate directly the metabolic activity of a biological system, the aim of this study was to use microcalorimetry as an additive method for sperm metabolism evaluation of cooled equine semen. Two ejaculates of four stallions were collected and motility, viability (eosin 3%) and membrane functional integrity (hyposmotic swelling test) of spermatozoa were evaluated. Sperm samples were processed following different protocols and the metabolism of these samples was accessed by calorimetry. Centrifugation is part of some of these processing protocols and although this procedure has been deleterious for sperm viability and plasma membrane integrity, no decrease in sperm motility was observed. Microcalorimetry was capable of detecting the positive effect of re-suspending the sperm pellet with Kenney extender. Thus, the use of microcalorimetry offered additional information for equine sperm metabolism evaluation and was efficient in detecting important information from sperm cell metabolism.

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“…The semen was then diluted with Kenney extender (Kenney et al. , 1975) to obtain samples containing a sperm number of approximately 1 × 10 8 sperm ml −1 (Vasconcelos et al. , 2008).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Metabolic evaluation of cooled equine spermatozoa

Vasconcelos¹,

Santana

Santos

et al. 2010

Andrologia

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show abstract

“…Com a diminuição dos substratos no meio ocorre o aumento dos catabólitos conforme relatado por Vasconcelos e colaboradores [17], com possível liberação de radicais livres, o que promoveria uma maior queda na motilidade espermática [18], com ação principal sobre as mitocôndrias presentes na peça intermediaria.…”

Section: Resultsunclassified

Efeito da adição do extrato de Jabuticaba (Myrciaria cauliflora) e Noni (Morinda citrifolia) ao sêmen bovino in natura e pós descongelado – Análise Preliminar / Effect of the addition of Jabuticaba (Myrciaria cauliflora) and Noni (Morinda citrifolia) extract to bovine semen in natura and post-thaw - preliminary analysis

Pimenta¹,

Furquim²,

Batista³

et al. 2021

Braz. J. Anim. Environ. Res.

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No intuito de melhorar os índices de viabilidade espermática após os processos de criogenia, pesquisadores buscam desenvolver diluidores que minimizem as injurias causadas pela técnica. O objetivo do presente trabalho foi avaliar a viabilidade dos espermatozoides bovinos fresco e pós descongelado após a adição de um diluidor comercial enriquecido com extratos de plantas. Para avaliação a fresco, foram utilizados 16 ejaculados in natura obtidos de oito touros de raças Zebuínas cujo sêmen foi coletado com vagina artificial. Para análise pós descongelamento, 16 palhetas de sêmen foram obtidas a partir destes mesmos animais e congeladas por período de 30 dias, sendo descongeladas por banho maria. Os experimentos foram definidos como sêmen a fresco e pós descongelamento seguindo dos seguintes grupos experimentais: Tryladyl®, somente o diluidor; Tryladyl® Jabuticaba, utilizando extrato aquoso da casca da jabuticaba (Myrciaria cauliflora) e Tryladyl® Noni, utilizando extrato aquoso de Noni (Morinda citrifolia). Foram avaliados motilidade e vigor. Para a análise estatística foi realizado a análise de variância (ANOVA), seguida pelo comparativo de médias Tukey, ao nível de significância de 0,05%. Na análise dos dados não foi observado diferença (p0,05) entre o diluidor comercial e os grupos adicionados os extratos de plantas. Houve diferença apenas quando estes foram comparados as amostras a fresco e pós descongelado, respectivamente (p0,05). A utilização de extratos de plantas com ação antioxidantes ou ativadora são promissores para melhorar a resposta de células espermáticas.

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