Determination of Optimum TBARS Conditions for Evaluation of Cow and Sheep Milk Oxidative Stability

Oancea, Alexandra-Gabriela; Untea, Arabela Elena; Dragomir, C.; Radu, Gabriel Lucian

doi:10.3390/app12136508

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Nevertheless, studies examining milk showed that sheep milk contained a higher PUFA content (~25%) compared with cow milk, leading to a higher risk for lipid peroxidation and consequently to a higher MDA content. Indeed, the MDA content in sheep compared with cow milk was higher (13.40 vs. 8.07%) after 24h of storage at room temperature [ 49 ]. Moreover, the same researchers observed a strong positive correlation between PUFA and the index of lipid oxidation (MDA) [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, the MDA content in sheep compared with cow milk was higher (13.40 vs. 8.07%) after 24h of storage at room temperature [ 49 ]. Moreover, the same researchers observed a strong positive correlation between PUFA and the index of lipid oxidation (MDA) [ 49 ]. Additionally, the total antioxidant capacity, determined using ORAC assay, of low-fat pasteurized milk was significantly lower (13.624–13.984 μmol TE/l) than that of milk with a higher fat content (14.124–14.216 μmol TE/l) [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Compositional Differences of Greek Cheeses of Limited Production

Pappa¹,

Kondyli²,

Pappas

et al. 2023

Foods

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Greece has a long tradition in cheesemaking, with 22 cheeses registered as protected designation of origin (PDO), 1 as protected geographical indication (PGI), and 1 applied for PGI. Several other cheeses are produced locally without any registration, which significantly contribute to the local economy. The present study investigated the composition (moisture, fat, salt, ash, and protein content), color parameters, and oxidative stability of cheeses that do not have a PDO/PGI certification, purchased from a Greek market. Milk and cheese types were correctly assigned for 62.8 and 82.1 % of samples, respectively, through discriminant analysis. The most important factors for milk type discrimination were L, a and b color attributes, salt, ash, fat-in-dry-matter, moisture-in-non-fat-substance, salt-in-moisture, and malondialdehyde contents, whereas a and b, and moisture, ash, fat, moisture-in-non-fat substance contents, and pH were the most influential characteristics for sample discrimination according to cheese type. A plausible explanation may be the differences in milk chemical composition between three animal species, namely cows, sheep, and goats and for the manufacture procedure and ripening. This is the very first report on the proximate analysis of these, largely ignored, chesses aiming to simulate interest for further study and production valorization.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Compositional Differences of Greek Cheeses of Limited Production

Pappa¹,

Kondyli²,

Pappas

et al. 2023

Foods

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show abstract

“…The TBARS assay is a rapid and convenient method to determine the levels of oxidative products derived from fatty acids in animal products ( Oancea et al, 2022 ). In agreement with the yield percentages, the TBARS values of CON remained consistent throughout the purification process involving various deodorants ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Characteristics of Purified Horse Oil by Supercritical Fluid Extraction with Different Deodorants Agents

Anneke,

Kim,

Kim

et al. 2024

Food Sci Anim Resour

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This study investigated the impact of activated carbon, palm activated carbon, and zeolite on horse oil (HO) extracted from horse neck fat using supercritical fluid extraction with deodorant-untreated HO (CON) as a comparison. The yield and lipid oxidation of deodorant untreated HO (CON) were not significantly affected by the three deodorants. However, deodorant-treated HOs exhibited significantly elevated levels of α-linolenic acid (C18:3n3) and eicosenoic acid (C20:1n9) compared to CON (p<0.05), while other fatty acids remained consistent. Zeolite-purified HO demonstrated significantly lower levels of volatile organic compounds (VOCs) than other treatments (p<0.05). Remarkably, zeolite decreased the concentration of pentane, 2,3-dimethyl (gasoline odor), by over 90%, from 177.17 A.U. ×10 6 in CON to 15.91 A.U. ×10 6 . Zeolite also effectively eliminates sec-butylamine (ammonia and fishy odor) as compared to other deodorant-treated HOs (p<0.05). Additionally, zeolite reduced VOCs associated with the fruity citrus flavor, such as nonanal, octanal, and D-limonene in HO (p<0.05). This study suggests that integrating zeolite in supercritical fluid extraction enhances HO purification by effectively eliminating undesirable VOCs, presenting a valuable approach for producing high-quality HO production in the cosmetic and functional food industries.

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“…For the quantitative analysis of MDA, the MDA standard was prepared by hydrolysis of TMP. 42 TMP standard solutions ranging from 0.36 to 10.8 μM were prepared with 0.1 M HCl. The solutions were hydrolyzed at 90 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Abiotic Signals on the Accumulation of Saponarin in Barley Leaves in Hydroponics Under Artificial Lights

Lee,

Kang,

Kim

et al. 2024

ACS Omega

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Functional flavonoid production is a new agenda in the agricultural industry, and young barley leaves (YBL) are one of the highlighted crops due to their health-beneficial flavonoid, saponarin. For the year-round cultivation of a high saponarin content of YBL, abiotic signal effects on the biosynthesis and metabolism in YBL need to be understood clearly. In this research, the effects of reactive oxygen species (ROS)-related abiotic signals, such as light, potassium, and sodium, were investigated on the biosynthetic metabolism in YBL cultivation under artificial lights. A higher quantity of blue-rich white light (6500 K of light temperature) irradiation enhanced ROS levels and the related enzyme activities (APX and CAT), as well as photosynthesis and saponarin amount, while red-rich white light (3000 K of light temperature) increased the photosynthesis only. In addition, 1.0 g L −1 K + treatment in water slightly reduced ROS levels and increased saponarin accumulation in YBL. These blue-rich light and K + supplemental conditions relatively increased OGT expression and reduced 4-coumaric acid and isovitexin as saponarin precursors. Furthermore, the relative ratio of lutonarin as an oxidized product of saponarin increased in increments of light quantity. Finally, the abiotic conditions for saponarin production were optimized with the mixture solution treatment of 1.0 g L −1 Na + and 1.0 g L −1 K + under 500 PPFD of 6500 K light, and the saponarin amount per leaf was 219.5 μg plant −1 ; it was comparable amount with that under sunlight condition.

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Determination of Optimum TBARS Conditions for Evaluation of Cow and Sheep Milk Oxidative Stability

Cited by 8 publications

References 25 publications

Compositional Differences of Greek Cheeses of Limited Production

Compositional Differences of Greek Cheeses of Limited Production

Characteristics of Purified Horse Oil by Supercritical Fluid Extraction with Different Deodorants Agents

Effect of Abiotic Signals on the Accumulation of Saponarin in Barley Leaves in Hydroponics Under Artificial Lights

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