Determination of Orbifloxacin in Rabbit Plasma by High-Performance Liquid Chromatography with Fluorescence Detection

García, María Ángeles; Solans, C.; Aramayona, J. J.; Rueda, S.; Bregante, M. A.

doi:10.1093/chromsci/37.6.199

Cited by 11 publications

(3 citation statements)

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“…Fluoroquinolones are a group of synthetic antibiotics chemically related to nalidixic acid. These compounds have been developed for the treatment of a variety of microbial infections in both animal and human medicine (Abadia et al, 1995;Garcia et al, 1999a;McKellar et al, 1999;Bregante et al, 1999;Perez et al, 2002). These agents work by inhibiting the action of DNA gyrase, interfering with the supercoiling of antibacterial chromosomal material (Cornett and Wentland, 1985).…”

Section: Introductionmentioning

confidence: 99%

Determination of enro?oxacin and its primary metabolite, cipro?oxacin, in pig tissues. Application to residue studies

García

Solans

Calvo

et al. 2005

Biomed. Chromatogr.

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A simple and sensitive HPLC method has been developed for the simultaneous determination of enrofloxacin (ENR) and ciprofloxacin (CIP) in pig tissue using difloxacin (DIF) as internal standard. Tissue sample preparations were carried out by adding phosphate buffer (pH 7.4, 0.1 m), followed by extraction with trichloromethane. Fluoroquinolones were separated on a reversed-phase column and eluted with aqueous buffer solution-acetonitrile (80:20, v/v). The concentrations of CIP, ENR and DIF eluted from the column, with retention times of 2.20, 2.73 and 4.38 min, respectively, were monitored by fluorescence detection at lambda(ex) 276 and lambda(em) 442 nm. The detection and quantitation limit were 8 and 25 ng/g, respectively, for both compounds. Standard curves were linearly related to concentration in the range 25-400 ng/g. The consequences of the introduction of minor reasonable variations (ruggedness studies) have also been analysed. Finally, the measurement of the tissue levels of ENR and CIP in the pig tissues after oral administration confirmed the utility of the proposed method.

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Section: Introductionmentioning

confidence: 99%

Determination of enro?oxacin and its primary metabolite, cipro?oxacin, in pig tissues. Application to residue studies

García

Solans

Calvo

et al. 2005

Biomed. Chromatogr.

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“…To date, methods reported in the literature for the determination of ORLX include liquid chromatography with ultraviolet [12][13][14], fluorimetry [12,[15][16][17], or mass spectrometry as detection techniques [16][17][18]. In another approach, sequential injection analysis coupled to solid-phase spectroscopy was utilized to develop a terbium-sensitized luminescent optical sensor for the analysis of ORLX in different matrices [19].…”

Section: Scheme 1 Chemical Structure Of Orbifloxacin (Orlx)mentioning

confidence: 99%

Application of Samarium- and Terbium-Sensitized Luminescence via a Multivariate-Based Approach for the Determination of Orbifloxacin

Al-Hashimi

El-Shafie

Jumaa

et al. 2022

Journal of Chemistry

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A lanthanide-based optical sensor has been developed for the sensitive and reliable spectrofluorometric determination of the fluoroquinolone antibiotic orbifloxacin (ORLX). Reaction of ORLX and two lanthanide metal ions, Sm(III) and Tb(III), in aqueous buffered solution produced highly fluorescent complexes. Plackett–Burman design (PBD) was used to explore the impact of four factors, pH, temperature (Temp), contact time (CT), and metal volume (MV), on the fluorescence intensity (FI) of the produced complexes. The obtained data showed that pH was the most significant variable. A blend of pH = 5.0, MV = 2.0 mL, T = 25°C, and CT = 10 min was used to achieve the maximum FI. FT-IR and Raman analyses were performed for the crystals of the as-prepared complexes. Obtained data showed shifting in most of the absorption bands, confirming the complexation of ORLX with both metal ions. Job’s method showed that the stoichiometry for the reaction of ORLX with Sm(III) and Tb(III) was 1 : 1. The proposed method was validated following the ICH guidelines. Injection formulation was analyzed successfully with the developed method with high recovery (99.42–100.91%). The detection and quantification limits were 0.987 and 3.289 ng/mL for the ORLX-Sm(III) complex and 1.020 and 3.399 ng/mL for the ORLX-Tb(III) complex, respectively.

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“…To date, all analytical methods described in literature for the determination of orbifloxacin in biological and other matrices involve liquid chromatography with UV detection [3,[7][8][9][10][11][12][13], *Address correspondence to this author at the School of Pharmacy, Faculty of Pharmaceutical Sciences, São Paulo State University, Araraquara, Sao Paulo, Brazil; Tel/Fax: +55 16 33016960; E-mail: salgadoh@fcfar.unesp.br fluorimetry detection [13][14][15][16][17][18][19][20][21][22] or mass spectrometry [16][17][18][19][20]23] and a spectroscopic technique coupled to a flow system [24] as detection techniques.…”

Section: Introductionmentioning

confidence: 99%

A Validated Stability-Indicating LC Method for Orbifloxacin in the Presence of Degradation Products

Cazedey¹,

Othman²,

Garg³

et al. 2011

CPA

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A stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of orbifloxacin in tablet dosage forms. Reversed-phase chromatography was performed on a Symmetry Shield RP-18 Waters ® , 250mm 4.6mm i.d., 5 m particle size column with 5% acetic acid:methanol (80:20, v/v) as mobile phase at a flow rate of 0.7 ml/min. PDA detection was performed at 290 nm. A well resolved peak was obtained for orbifloxacin at a retention time of approximately 7.9 min. For stress studies the drug was subjected to oxidation, hydrolysis, photolysis and thermal degradation. The developed method was validated as per ICH guidelines for the parameters such as system suitability, linearity, limit of quantification (LOQ), limit of detection (LOD), precision, accuracy, selectivity and robustness. Results obtained were found to be satisfactory. No interference from any excipients in the pharmaceutical dosage form or degradation products was observed.

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Determination of Orbifloxacin in Rabbit Plasma by High-Performance Liquid Chromatography with Fluorescence Detection

Cited by 11 publications

References 0 publications

Determination of enro?oxacin and its primary metabolite, cipro?oxacin, in pig tissues. Application to residue studies

Determination of enro?oxacin and its primary metabolite, cipro?oxacin, in pig tissues. Application to residue studies

Application of Samarium- and Terbium-Sensitized Luminescence via a Multivariate-Based Approach for the Determination of Orbifloxacin

A Validated Stability-Indicating LC Method for Orbifloxacin in the Presence of Degradation Products

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