Determination of organic acids by CE and CEC methods

Klampfl, Christian W.

doi:10.1002/elps.200700159

Cited by 38 publications

(26 citation statements)

References 133 publications

(166 reference statements)

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“…In contrast, the salvage pathway for pyrimidine nucleotides occurs at the level of the nucleosides uridine and cytidine. To date, more than 30 defects of the metabolism of purines and pyrimidines have been described [16]. Some of these genetic enzyme defects have mild or even asymptotic phenotypes under normal conditions, but can have serious clinical consequences by adverse reactions to medication.…”

Section: Purines and Pyrimidinesmentioning

confidence: 99%

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

Kulik

Kuilenburg

2012

Analytical Techniques for Clinical Chemistry

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Section: Purines and Pyrimidinesmentioning

confidence: 99%

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

Kulik

Kuilenburg

2012

Analytical Techniques for Clinical Chemistry

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“…Separation of the acidic solutes was often carried out in ion-suppressed mode of CEC. However, it is relatively difficult because the analytes tend to migrate against the EOF [30]. The use of MP-GMA-EDMA column that generates anodic EOF makes it possible to overcome this problem and exert additional selectivities.…”

Section: Electrochromatographic Performance On the Monolithic Columnmentioning

confidence: 99%

Macrocyclic polyamine‐modified poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monolith for capillary electrochromatography

Tian¹,

Fang-Xing²,

Yang³

et al. 2008

Electrophoresis

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Macrocyclic polyamine-modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith for capillary electrochromatography 1,4,10,13,,17-dione (macrocyclic polyamine)-modified polymer-based monolithic column for CEC was prepared by ring opening reaction of epoxide groups from poly(glycidyl methacrylate-co-ethylene dimethacrylate) (GMA-co-EDMA) monolith with macrocyclic polyamine. Conditions such as reaction time and concentration of macrocyclic polyamine for the modification reaction were optimized to generate substantial EOF and enough chromatographic interactions. Anodic EOF was observed in the pH range of 2.0-8.0 studied due to the protonation of macrcyclic polyamine at the surface of the monolith. Morphology of the monolithic column was examined by SEM and the incorporation of macrocyclic polyamine to the poly(GMA-co-EDMA) monolith was characterized by infrared (IR) spectra. Successful separation of inorganic anions, isomeric benzenediols, and benzoic acid derivatives on the monolithic column was achieved for CEC. In addition to hydrophobic interaction, hydrogen bonding and electrostatic interaction played a significant role in the separation process.

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“…Low robustness is not only a problem when new BGEs have to be done, as small pH or concentration differences will induce changes in the calibration curve and the LOQ, but also during successive runs, as buffer depletion will modify the BGE composition in the BGE vials leading to changes in the TR. Indeed lower repeatability is often observed when using reverse detection mode in comparison to a direct counterpart [18]. Thus, the aim of this work is to study the robustness of the TR, and investigate if low robustness could be a problem in reverse detection mode.…”

Section: Introductionmentioning

confidence: 99%

Robustness of the co‐ion transfer ratio in capillary electrophoresis

Erny¹,

Esteves

2009

J of Separation Science

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In CE, indirect detection mode often exhibits a lower precision than its direct counterpart. Although various explanations have already been advanced, in this work, we aimed to investigate if this is due, in part, to problems of robustness of the co-ion transfer ratio (TR), thus being inherent to this particular detection scheme. This was investigated using simulation software that allows an accurate control of various parameters and validated using acetic acid as a test compound. It was conclusively demonstrated that the TR could vary by more than 6% when the concentration of one of the ions in the BGE was changed by as few as 1%. The presence of a system peak seems to be particularly damaging as it has been shown that the TR of peaks whose mobilities differ by more than 0.5x10(-8) m(2) V(-1) s(-1) from one of the system peaks, still have a relatively low robustness.

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Determination of organic acids by CE and CEC methods

Cited by 38 publications

References 133 publications

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

Macrocyclic polyamine‐modified poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monolith for capillary electrochromatography

Robustness of the co‐ion transfer ratio in capillary electrophoresis

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