Determination of organic acids in sugar cane process juice by high-performance liquid chromatography: improved resolution using dual aminex HPX-87H cation-exchange columns equilibrated to different temperatures

Blake, J. D.; Clarke, Marilyn L.; Richards, Geoffrey N.

doi:10.1016/s0021-9673(01)96512-4

Cited by 49 publications

(11 citation statements)

References 32 publications

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“…The HPLC column employed for the separation and identification of organic carbonates is a strong acid cation‐exchange column with a polystyrene‐divinylbenzene resin backbone and exchangeable hydrogen ion (H + ) as the counter ion . This single column employs multiple modes of interactions such as reverse phase, normal phase, ion exclusion, ion exchange, ligand exchange and size exclusion between the analyte and the mobile and stationary phase for separating the analytes.…”

Section: Resultsmentioning

confidence: 99%

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Bhalkikar

Marin

Cheung

2016

J of Separation Science

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An ion-moderated partition high-performance liquid chromatography method was developed for the separation and identification of common organic carbonates. The separation of organic carbonates was achieved on an ion exclusion column with an exchangeable hydrogen ion. An isocratic, aqueous mobile phase was used for elution and detection was performed with a refractive index detector. The developed method was validated for specificity, linearity, limits of detection and quantification, precision and accuracy. All calibration curves showed excellent linear regression (R > 0.9990) within the testing range. The limits of detection were 3.8-30.8 ppm for the analyzed carbonates. Improvements in the peak resolution of the chromatograms were achieved by decreasing the column temperature. Addition of the organic modifier, acetonitrile, to the eluent was found to have insignificant effects on the peak resolution. The developed method was demonstrated for analyzing organic carbonate components in the electrolyte system of a commercial lithium ion battery.

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Section: Resultsmentioning

confidence: 99%

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Bhalkikar

Marin

Cheung

2016

J of Separation Science

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“…The lactate and diacetate concentrations were determined by ABC research laboratories (Gainesville, Fla., U.S.A.). The reference used for the method is “JOC () 398:265” (Blake and others ).…”

Section: Methodsmentioning

confidence: 99%

Survival and Metabolic Activity of Listeria monocytogenes on Ready‐to‐Eat Roast Beef Stored at 4 °C

Broady

Han

Yuan

et al. 2016

Journal of Food Science

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Three brands of commercial roast beef were purchased and artificially inoculated with a 5-strain Listeria monocytogenes cocktail at 2 inoculation levels (approximately 3 and 6 Log CFU/g). Although all 3 brands contained sodium diacetate and sodium lactate, inoculated Listeria cocktail survived for 16 d in all 3 brands; significant increases in L. monocytogenes numbers were seen on inoculated Brand B roast beef on days 12 and 16. Numbers of L. monocytogenes increased to 4.14 Log CFU/g for the 3 Log CFU/g inoculation level and increased to 7.99 Log CFU/g for the 6 Log CFU/g inoculation level by day 16, with the pH values being 5.4 and 5.8 respectively. To measure the cell viability in potential biofilms formed, an Alamar blue assay was conducted. Brand B meat homogenate had the highest metabolic activities (P < 0.05). By comparing its metabolic activities to Brands A and C and the inoculated autoclaved meat homogenates, results indicated that the microflora present in Brand B may be the reason for high metabolic activities. Based on the denaturing gradient gel electrophoresis and the Shannon-Wiener diversity index analysis, the "Brand" factor significantly impacted the diversity index (P = 0.012) and Brand B had the highest microflora diversity (Shannon index 1.636 ± 0.011). Based on this study, results showed that antimicrobials cannot completely inhibit the growth of L. monocytogenes in ready-to-eat roast beef. Native microflora (both diversity and abundance), together with product formula, pH, antimicrobial concentrations, and storage conditions may all impact the survival and growth of L. monocytogenes.

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“…One of the major problems experienced with the use of these columns are related to the co-elution of non-acid components and the poor resolution of the chromatographic peaks, thus limiting their use. Moreover, the ion exchange's separation mechanism implies that organic acids should be in their ionic form, so that a severe control of pH is required [116].…”

Section: Phenolics and Organic Acidsmentioning

confidence: 99%

Analytical Methods for Extraction and Identification of Primary and Secondary Metabolites of Apple (Malus domestica) Fruits: A Review

Acquavia

Pascale²,

Foti

et al. 2021

Separations

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Apples represent a greater proportion of the worldwide fruit supply, due to their availability on the market and to the high number of existing cultivar varieties and apple-based products (fresh fruit, fruit juice, cider and crushed apples). Several studies on apple fruit metabolites are available, with most of them focusing on their healthy properties’ evaluation. In general, the metabolic profile of apple fruits strongly correlates with most of their peculiar characteristics, such as taste, flavor and color. At the same time, many bioactive molecules could be identified as markers of a specific apple variety. Therefore, a complete description of the analytical protocols commonly used for apple metabolites’ characterization and quantification could be useful for researchers involved in the identification of new phytochemical compounds from different apple varieties. This review describes the analytical methods published in the last ten years, in order to analyze the most important primary and secondary metabolites of Malus domestica fruits. In detail, this review gives an account of the spectrophotometric, chromatographic and mass spectrometric methods. A discussion on the quantitative and qualitative analytical shortcomings for the identification of sugars, fatty acids, polyphenols, organic acids, carotenoids and terpenes found in apple fruits is reported.

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Determination of organic acids in sugar cane process juice by high-performance liquid chromatography: improved resolution using dual aminex HPX-87H cation-exchange columns equilibrated to different temperatures

Cited by 49 publications

References 32 publications

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Method development for separating organic carbonates by ion‐moderated high‐performance liquid chromatography

Survival and Metabolic Activity of Listeria monocytogenes on Ready‐to‐Eat Roast Beef Stored at 4 °C

Analytical Methods for Extraction and Identification of Primary and Secondary Metabolites of Apple (Malus domestica) Fruits: A Review

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