Determination of oxiracetam in plasma and urine by column-switching high-performance liquid chromatography

Lecaillon, J.B.; Souppart, C.; Duigou, F. Le; Dubois, J.-P.

doi:10.1016/0378-4347(89)80021-0

Cited by 11 publications

(2 citation statements)

References 5 publications

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“…The analytical tools developed for the determination of oxiracetam in biological matrices include normal-phase liquid chromatography with UV detection [5], reversed-phase liquid chromatography with fluorimetric detection combined with liquid phase extraction [6], and column-switching high-performance liquid chromatography [7]. These reported methods, however, are not adequate for pharmacokinetic studies due to relatively lower selectivity and sensitivity of short-wavelength UV detection system and complex sample pretreatment.…”

Section: Introductionmentioning

confidence: 98%

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Son

Lee

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of oxiracetam in human plasma. Oxiracetam, a highly polar compound, was successfully retained by Atlantis dC18 reversed-phase column and detected with triple-quadrupole tandem mass spectrometry. After addition of internal standard (piracetam) to human plasma, plasma was simply precipitated with two volume of acetonitrile, evaporated and dissolved in 0.1% acetic acid. This method for the determination of oxiracetam was accurate and reproducible, with a limit of quantitation of 0.2microg/ml in human plasma. The standard calibration curve for oxiracetam was linear (r(2) = 0.999) over the concentration range 0.2-40.0microg/ml in human plasma. The intra- and inter-day precision over the concentration range of oxiracetam was lower than 8.3% (relative standard deviation, %R.S.D.), and accuracy was between 92.5 and 106.4%.

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Section: Introductionmentioning

confidence: 98%

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Son

Lee

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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“…This drug is also known to improve both learning and memory processes [1][2][3][4]. The analytical tools developed for the determination of oxiracetam in biological matrices include normal-phase liquid chromatography with UV detection [5], reversed-phase liquid chromatography with fluorescence detection followed by derivatization [6], and column-switching high-performance liquid chromatography [7]. These reported methods, however, are not adequate for pharmacokinetic studies due to relatively lower selectivity and sensitivity of short-wavelength UV detection system and tedious sample pretreatment, such as a fluorescent derivatization.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Son

Lee

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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Pharmacokinetics of oxiracetam in elderly patients after 800 mg oral doses, comparison with non-geriatric healthy subjects

Lecaillon¹,

Dubois²,

Coppens³

et al. 1990

Eur. J. Drug Metab. Pharmacokinet.

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The pharmacokinetics of oxiracetam have been studied in eighteen elderly patients and in six healthy non-geriatric adults. A 800 mg single oral dose was administered in the morning of the first day and repeatedly, every 12 h, from day 2 evening to day 10 morning, to the elderly patients. The healthy non-geriatric adults were given a 800 mg single oral dose of oxiracetam. In healthy non-geriatric subjects after a single oral administration of 800 mg, the normalized plasma levels of oxiracetam for 1 mg/kg dose were similar to those already recorded after a 2000 mg single dose of oxiracetam. Therefore, there was no tendency towards non-linear pharmacokinetics of oxiracetam between 800 and 2000 mg single doses in healthy subjects. After the single oral dose, the mean area under the plasma concentration-time curve of oxiracetam in elderly patients was increased by a factor of two as compared to that observed in non-geriatric healthy subjects whereas the maximum concentration (Cmax) was almost not modified and slightly delayed. This can be explained by a slower absorption and elimination in the elderly patients. The highest oxiracetam levels were predominantly recorded in the oldest patients. The slower elimination (mean T1/2 = 12.3 h in elderly and 7.7 h in healthy subjects) could be attributed to a physiological decrease of the renal function. The volume of distribution was not significantly modified in the elderly patients.(ABSTRACT TRUNCATED AT 250 WORDS)

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Determination of oxiracetam in plasma and urine by column-switching high-performance liquid chromatography

Cited by 11 publications

References 5 publications

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Rapid quantitative analysis of oxiracetam in human plasma by liquid chromatography/electrospray tandem mass spectrometry

Pharmacokinetics of oxiracetam in elderly patients after 800 mg oral doses, comparison with non-geriatric healthy subjects

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