Determination of oxolinic acid residues in the muscle tissue of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay

Abstract

This study was designed to determine oxolinic acid (OA) residues in the muscle of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay (LFIA) and ultra-performance liquid chromatography coupled with fluorescence detection (UPLC-FLD). After 25 cultured olive flounder were treated by dipping in water dosed with OA (50 mg/L water for 1 h), muscle tissues were sampled and prepared for the detection of OA residues. The visual detection limit of the test strip for OA determined by the C/T ratio of 1 was 28 µg/kg of muscle. The detection limit of OA based on the standard curve was 4 and 1.6 µg/kg of muscle using LFIA and UPLC-FLD, respectively, and recoveries of all spiked samples by LFIA and UPLC-FLD were >96%. LFIA and UPLC-FLD showed good correlation (R 2 > .9997) for a series of samples collected over 5 days post treatment. The LFIA is applicable to the detection of OA in the muscle tissue of farmed fish, and is an effective and economical method that will enable high-throughput screening.

Oxolinic Acid Generated Green Fluorescence Based on a Terbium-Functionalized Covalent Organic Framework

“…So far, the numbers of technologies have been established for OXO including high-performance liquid chromatography (HPLC), cyclic voltammetry (CV), spectrofluorometric, and so on. − Although these technologies have excellent veracity, most of them are generally expensive and laborious, especially they are also time-consuming . These shortcomings have impeded their application in the routine detection of OXO.…”

Effect of pH on Adsorption of Tetracycline Antibiotics on Graphene Oxide

“…Currently, adverse reactions caused by antibiotics in the environment have become a problem of global concern. Tetracycline antibiotics are the most widely used antibiotics, commonly found in domestic sewage, medical wastewater, animal feed, and aquaculture wastewater discharge [1,2]. Research has been reported on eliminating antibiotics through membrane filtration, oxidation, photocatalysis, and biodegradation, which are costly, timeconsuming, and complex [3].…”

“…of quasi primary kinetic model and quasi secondary kinetic model for the adsorption of TCs by GO at different pH values.AdsorbatespH qe,exp (mg/g) Pseudo-Second-Order Pseudo-First-Order k2 q e (mg/g) R 2 k 1 q e (mg/g) R2 …”