Determination of p53 biomarker using an electrochemical immunoassay based on layer-by-layer films with NiFe2O4 nanoparticles

Ibáñez-Redín, Gisela; Joshi, Nirav; Nascimento, Gustavo Freitas do; Wilson, Deivy; Melendez, Matias Eliseo; Carvalho, André Lopes; Reis, Rui Manuel; Gonçalves, Débora; Oliveira, Osvaldo N.

doi:10.1007/s00604-020-04594-z

Cited by 24 publications

(17 citation statements)

References 50 publications

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“…Among spinel ferrites, nickel ferrite (NiFe 2 O 4 ) is a soft magnetic material with low coercivity, high saturation magnetization, chemical stability, and electrical resistivity [26,27]. Hence, it is suitable for several technological applications such as electrochemical sensors [28][29][30], supercapacitor [31], adsorbent [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

Zhou

et al. 2021

Engineering in Life Sciences

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The NiFe2O4 magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy. The immobilization and purification of His‐tagged GluDH on NF‐MNPs were investigated. The optimal immobilization conditions were obtained that mixed cell lysis and carriers in a ratio of 0.13 in pH 8.0 Tris‐HCl buffer at 30℃ and incubated for 2 h. The highest activity recovery and protein bindings were 71.39% and 38.50 μg mg–1 support, respectively. The immobilized GluDH exhibited high thermostability, pH‐stability and it can retain more than 65% of the initial enzyme after 10 cycles for the conversion of glucose to gluconolactone. Comparing with a commercial Ni‐NTA resin, the NF‐MNPs displayed a higher specific affinity with His‐tagged recombinant GluDH.

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Section: Introductionmentioning

confidence: 99%

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

Zhou

et al. 2021

Engineering in Life Sciences

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“…128 A 6 1,0 × 10 −3 mol L -1 em tampão fosfato 0,1 mol L −1 pH 7. v=100 mV s −1 . Fonte: Adaptada de IBAÑEZ-REDÍN et al 126 Por outro lado, a modificação do eletrodo com NPs funcionalizadas levou à diminuição nas respostas de corrente (Figura 3.5). Este efeito pode ser justificado pela presença de grupos funcionais com carga negativa na superfície das partículas e que podem repelir a sonda redox, também carregada negativamente.…”

Section: 2unclassified

“…6 1,0 × 10 −3 mol L -1 em tampão fosfato 0,1 mol L −1 pH 7. Parâmetros dos voltamogramas de pulso diferencial: tempo de modulação = 0,05 s, amplitude de modulação = 0,09 V e incremento de potencial = 0,05 V. Fonte: Adaptada de IBAÑEZ-REDÍN et al 126…”

Section: 2unclassified

“…125 Figura 3.8 -Espectros PM-IRRAS para superfícies de Au modificadas com MUA após a deposição de PEI e NPs funcionalizadas (A) e após a imobilização de anticorpos anti-p53 usando baixa (Au/PEI/NPs-Ab A) e alta (Au/PEI/NPs-Ab-B) concentração de NPs funcionalizadas. Fonte: Adaptada de IBAÑEZ-REDÍN et al 126 Os efeitos da imobilização dos anticorpos monoclonais anti-p53 nas superfícies modificadas usando baixas (Au/PEI/NPs-Ab A) e altas (Au/PEI/NPs-Ab B) concentrações de NPs funcionalizadas foram estudados por meio de espectros de PM-IRRAS. Em ambos casos, foram subtraídos os espectros dos filmes contendo PEI e NPs, com o objetivo de observar mais claramente a contribuição das bandas relacionas com os anticorpos.…”

Section: Imobilização Dos Anticorpos Anti-p53unclassified

“…138 Além disto, o uso de uma maior concentração de NPs também influenciou de maneira positiva na repetibilidade de fabricação dos imunossensores, que foi avaliada a partir de voltammograms de pulso diferencial obtidos para 5 eletrodos ,0 × 10 −3 mol L −1 em tampão fosfato 0,1 mol L −1 pH 7. Parâmetros dos voltamogramas de pulso diferencial: tempo de modulação = 0,05 s, amplitude de modulação = 0,09 V e incremento de potencial = 0,05 V. Fonte: Adaptada de IBAÑEZ-REDÍN et al 126 As principais características de ambas as plataformas, inferidas a partir dos experimentos de caracterização e das curvas de calibração na Figura 3.9, são comparadas na Tabela 3.2. A plataforma SPCE/PEI/NPs-Ab A apresentou maior sensibilidade devido principalmente ao maior sinal de corrente.…”

Section: Detecção De P53 Em Soluções Padrãounclassified

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Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

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Label-free electrochemical immunosensors based on screen-printed carbon electrodes are a promising tool for performing fast, simple and economical detection of cancer biomarkers.However, despite the potential of these devices, some limitations, such as their low sensitivity, need to be overcome before their widespread use as technology for clinical analysis. Herein, we proposed different strategies to modify screen-printed carbon electrodes, aiming to improve the analytical signal and the surface area for the immobilization of antibodies, which are two essential parameters for reaching good analytical performance in label-free immunosensors. We explored the application potential of carbon black and NiFe 2 O 4 nanoparticles, two low-cost nanomaterials with excellent electrochemical properties, which remain relatively unexplored in the construction of electrochemical immunosensors. In a first approach, an immunosensor was developed for detecting the carbohydrate antigen CA 19-9 using electrodes modified with composites of carbon black and the polyelectrolytes polyethyleneimine and polyacrylic acid. The immunosensor exhibited a low detection limit of 0.07 U mL − 1 , in addition to an excellent fabrication reproducibility, stability and selectivity.The proposed device showed good correlation with commercial immunoassays in the determination of CA 19-9 in cell lysates and human serum samples from pancreatic cancer patients. An immunosensor for detecting the tumor protein p53 was also developed, using electrodes modified with films of polyethyleneimine and NiFe 2 O 4 nanoparticles. The analytical parameters of the devices are among the best reported in the literature for determination of p53, with a low detection limit of 5 fg mL −1 . Also, the immunosensors showed an excellent selectivity toward p53 detection in fetal bovine serum, saliva and cell lysate samples. The platforms proposed herein combined the numerous advantages of screenprinted electrodes with the excellent properties of the studied nanomaterials, allowing the development of low-cost (˂BRL$ 4/ test) and disposable devices with excellent analytical performance, which can be adapted for the detection of several disease markers.

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Recent Advances in Semiconducting Nanowires‐Based Hybrid Structures for Solar Cell Application

Bhaliya

Popaliya²,

Patel

et al. 2022

1D Semiconducting Hybrid Nanostructures

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Determination of p53 biomarker using an electrochemical immunoassay based on layer-by-layer films with NiFe2O4 nanoparticles

Cited by 24 publications

References 50 publications

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

Recent Advances in Semiconducting Nanowires‐Based Hybrid Structures for Solar Cell Application

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