Determination of paclitaxel in biological fluids by micellar electrokinetic chromatography

Hempel, Georg; Lehmkuhl, Doris; Krümpelmann, Sebastian; Blaschke, Gottfried; Boos, Joachim

doi:10.1016/0021-9673(96)00351-2

Cited by 68 publications

(28 citation statements)

References 9 publications

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“…Taxoids have been analyzed by thin-layer chromatography (TLC) (Fujino et al, 2001), high-performance liquid chromatographyphotodiode array detection (HPLC-PAD) (Neméth-Klss et al, 1996), high-performance liquid chromatography-UV detection (HPLC-UV; Theodoridis et al, 1998), micellar electrokinetic capillary chromatography (MECC; Hempel et al, 1996) and high-speed counter-current chromatography (HSCCC; Cao et al, 1998). Short-term methods to determine taxoids have been described using HPLC-MS (Madhusudanan et al, 2002a,b;Kuppens et al, 2005;Mroczek et al, 2000;Mortier et al, 2004).…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

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Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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“…Particularly for the most widely used UV absorbance detection, the concentration detection limit is generally on the order of 10 -100-fold higher than that of HPLC due to the short optical pass-length. Improvement of the capillary geometry and optical design, such as use of a bent capillary (Z-cell) [3], a silver-coated capillary (multireflection flow cell) [4], a bubble-cell or extended light part capillary [5][6][7], end-column [8] and off-column [9] detections, is one approach to lower the detection limit of CE. Another effective approach is sample concentration [2].…”

Section: Introductionmentioning

confidence: 99%

Isoelectric focusing sample injection for capillary electrophoresis of proteins

Wu¹,

Zhang²,

Onoda³

2005

Electrophoresis

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Carrier ampholyte-free isoelectric focusing (IEF) sample injection (concentration) for capillary electrophoresis (CE) is realized in a single capillary. A short section of porous capillary wall was made near the injection end of a capillary by HF etching. In the etching process, an electric voltage was applied across the etching capillary wall and electric current was monitored. When an electric current through the etching capillary was observed, the capillary wall became porous. The etched part was fixed in a vial, where NaOH solution with a certain concentration was added during the sample injection. The whole capillary was filled with pH 3.0 running buffer. The inlet end vial was filled with protein sample dissolved in the running buffer. An electric voltage was applied across the inlet end vial and etched porous wall. A neutralization reaction occurs at the boundary (interface) of the fronts of H+ and OH-. A pH step or sharp pH gradient exists across the boundary. When positive protein ions electromigrate to the boundary from the sample vial, they are isoelectricelly focused at points corresponding to their pH. After a certain period of concentration, a high voltage is applied across the whole capillary and a conventional CE is followed. An over 100-fold concentration factor has been easily obtained for three model proteins (bovine serum albumin, lysozyme, ribonuclease A). Furthermore, the IEF sample concentration and its dynamics have been visually observed with the whole-column imaging technique. Its merits and remaining problem have been discussed, too.

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“…The reported methods, however, had some drawbacks. TLC [28,29], MECC [30,31] and high-speed counter-current chromatography (HSCCC) [32,33] all can separate taxoids, but they cannot adapt to mass production. Chemical reac-tion method was used to separate taxoids with the transformed structures [34,35], but it needs strict reaction condition and is difficult to control.…”

Section: Introductionmentioning

confidence: 99%

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography

Sun

et al. 2009

J of Separation Science

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Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography An environment-friendly method was established for the preparative separation and enrichment of four taxoids, namely 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), cephalomannine and paclitaxel from Taxus chinensis needles extracts. Characteristics of seven widely used macroporous resins for four taxoids were compared, AB-8 resin offered better adsorption and desorption capacities than others. AB-8 resin column chromatography was used to study the desorption process for four taxoids. The optimum parameters for desorption were 30% ethanol 5 RV for removing impurities, following 15 RV for 10-DAB III, after the desorption of impurities with 35% ethanol 10 RV, 45% ethanol 30 RV for 7-xyl-10-DAT, then 65% ethanol 10 RV for cephalomannine and paclitaxel, the flow rate was 6 RV/ h. After separation on AB-8 resin column chromatography, the contents of 10-DAB III, 7-xyl-10-DAT, cephalomannine and paclitaxel in the product reached 4.58, 13.17, 1.36 and 3.08%, respectively, which were 7.63-, 3.68-, 6.18-and 6.55-fold to those in T. chinensis needles extracts. The recovery yields were 94.96, 77.32, 88.09 and 95.25%. In general, the AB-8 resin column chromatography has the advantages of lower cost, high efficiency and simple procedure. Therefore, it may provide scientific references for the preparative separation and enrichment of taxoids from other T. species.

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Determination of paclitaxel in biological fluids by micellar electrokinetic chromatography

Cited by 68 publications

References 9 publications

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Isoelectric focusing sample injection for capillary electrophoresis of proteins

Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatography

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