Determination of para -aminobenzoic acid, a degradation product of procaine hydrochloride, by zero-crossing first-derivative spectrometry

Badea, Irinel Adriana; Moja, Dana; Vladescu, Luminita

doi:10.1007/s00216-002-1446-0

Cited by 13 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several methods have been reported for PABA analysis. They are based on spectrophotometry17 or luminescence,18–21 and sequential injection analysis 5, 6. For more complex samples, various LC methods have been developed with different detectors such as UV,22–27 fluorescence,16, 27, 28 and electrochemical detection 29, 30.…”

mentioning

confidence: 99%

Free and total para‐aminobenzoic acid analysis in plants with high‐performance liquid chromatography/tandem mass spectrometry

Zhang

Mortier

Storozhenko

et al. 2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

para-Aminobenzoic acid (PABA), a precursor in the synthesis of folates in plants, is determined by liquid chromatography/tandem mass spectrometry (LC/MS/MS). In plants PABA can be converted into its beta-D-glucopyranosyl ester (PABA-Glc) and can also exist in its free form. In this work, we developed and validated a quantitative method to study free and total PABA in plants. The total PABA (free PABA plus PABA-Glc) can be evaluated after acid hydrolysis at 80 degrees C for 2 hours. The plant material is homogenized and the PABA content is quantified using the standard addition procedure. The validated method is selective, sensitive, simple, accurate, has a recovery between 99.6 to 102.5%, is reproducible (RSD between 1.4 and 4.4%), and is linear between 2.5 and 1538 ng/mL. Free and total PABA determinations in five vegetables showed that different plant species had different amounts of free and total PABA, and that the ratios of total versus free PABA were also variable. This new method could be valuable for studies of folate synthesis in plants.

show abstract

mentioning

confidence: 99%

Free and total para‐aminobenzoic acid analysis in plants with high‐performance liquid chromatography/tandem mass spectrometry

Zhang

Mortier

Storozhenko

et al. 2005

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…The quantitative determination of organic compounds with whose structure ensures the existence of UV-VIS spectrum is easy to measure if is only one species in the mixture. In addition, if the sample to be analyzed contains several organic compounds and whose spectra is overlaped, depending on their structure, specific method called derived spectrometry (Badea et al 2002;Badea & Vladescu 2015; Procaine hydrochloride Procainamide hydrochloride Lidocaine Moldovan & Alexandrescu 2004;Zimmer & Czarnecki 2010) or multicomponent analysis can be developed to detected of them. In our experiment the absorption spectra of PH•HCl (11.04 µg/mL), PHA•HCl (10.72 µg/mL) and Lid (9.28 µg/mL) as well as their mixture (11.04 -10.72 -9.28 µg/mL) showed a high degree of overlapping in the range of 200-350 nm which made impossible to simultaneously determinate the PH•HCl, PHA•HCl and Lid (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of procaine hydrochloride, procainamide hydrochloride and lidocaine by molecular absorption spectrometry

et al. 2019

View full text Add to dashboard Cite

Two spectrometric methods have been developed for quantitative simultaneous determination of procaine hydrochloride (PH·HCl), procainamide hydrochloride (PHA·HCl) and lidocaine (Lid) from synthetic mixture. The methods employed are first derivative spectrometry, using zero crossing method and multicomponent analysis which is based on the additivity law. Using first derivative spectrometry, the wavelength selected for the quantitative determination of PH·HCl was 237 nm for Lid was 242 nm and for PHA·HCl was 290 nm in mixture. The method is linear when the concentration ranged between 6.62-9.93 μg/mL for PH·HCl, 6.43-9.64 for PHA·HCl and 5.56-8.35 for Lid. The multicomponent analysis is a direct method and involves the absorbance measurements of at three different wavelengths. The molar absorption coefficients values were calculated at each wavelength and the concentration of PH·HCl, PHA·HCl and Lid from mixture was determined by solving matrix using Cramer's rule. The recovery of each compound in mixture was calculated and it is 101.4 % for PH·HCl, 100.4 % for PHA·HCl and 98.4 % for Lid.

show abstract

“…Several other methods have also been reported in the literature for the determination of PABA based on colorimetric [9], spectrometric [10,11], chromatographic [12,13] and electrochemical [14,15] detection. Procaine has likewise been detected by various methods including polarimetry [16], HPLC [17,18], IR [19], fluorimetric [20] and Raman spectroscopy [21].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method

Dhananjeyan

Bykowski

Trendel

et al. 2007

Journal of Chromatography B

View full text Add to dashboard Cite

Determination of para -aminobenzoic acid, a degradation product of procaine hydrochloride, by zero-crossing first-derivative spectrometry

Cited by 13 publications

References 12 publications

Free and total para‐aminobenzoic acid analysis in plants with high‐performance liquid chromatography/tandem mass spectrometry

Free and total para‐aminobenzoic acid analysis in plants with high‐performance liquid chromatography/tandem mass spectrometry

Simultaneous determination of procaine hydrochloride, procainamide hydrochloride and lidocaine by molecular absorption spectrometry

Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method

Contact Info

Product

Resources

About