Determination of parthenin in Parthenium hysterophorus L. by means of HPLC-UV: Method development and validation

Hernández, Yanelis Saucedo; Sánchez, Luis Ramón Bravo; Bedia, Mirtha Mayra González; Gomez, Luis Torres; Rodríguez, Elisa Jorge; Miguel, Hilda M. González San; Mosquera, Dulce González; García, Livier Polín; Dhooghe, Liene; Theunis, Mart; Pieters, Luc; Apers, Sandra

doi:10.1016/j.phytol.2011.02.001

Cited by 9 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A HPLC method for the analysis of santonin was devised using parameters interpreted from previous publications [ 5 , 14 ]. The standard of santonin was run in the HPLC machine using a C18 column showing a retention time of approximately 5.7 minutes ( Fig 2 ).…”

Section: Resultsmentioning

confidence: 99%

Quantification of santonin in eight species of Artemisia from Kazakhstan by means of HPLC-UV: Method development and validation

et al. 2017

View full text Add to dashboard Cite

Santonin, a powerful anthelmintic drug that was formely used to treat worms, is Artemisia cina's main constituent. However, due to its toxicity to humans, it is no longer in use. Kazakhstan is looking to introduce this plant as an anthelmintic drug for veterinary purposes, despite the known toxic properties of the santonin. The objective of this study was to develop a fast and specific method for the identification of santonin and its precise quantitation using HPLC-UV in order to avoid unnecessary intoxication, which is paramount for the development of veterinary medicines. The results obtained showed that santonin appears at around 5.7 minutes in this very reliable HPLC method. The validation of the method was performed by the investigation of parameters such as precision, accuracy, reproducibility and recovery. The method was used to identify and quantify santonin in leaves of A. scoparia, A. foetida, A. gmelinni, A. schrenkiana, A. frigida, A. sublesingiana, A terra-albae, and A. absinthium from Kazakhstan as well as in three different extracts of leaves of A. cina. This study has provided a faster and simpler method for the identification and quantification of this compound in other species of Artemisia of economic importance.

show abstract

Section: Resultsmentioning

confidence: 99%

Quantification of santonin in eight species of Artemisia from Kazakhstan by means of HPLC-UV: Method development and validation

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Fourier Transform Infrared Spectroscopy offers a fast, valuable tool to identify and characterize the chemical constituents or functional groups present in the sample to elucidate the structural compounds [10,33], as was the case in identifying the isolated compounds. Our results of UHPLC-MS analyses showed the presence of major pseudo molecular ions in both samples, m/z 263 and 265, corresponding to parthenin and coronopolin, respectively; the molecular mass of the compounds of interest was used (262.31 g/mol for parthenin and 264.32 g/mol for coronopolin), along with an analysis in positive mode using electrospray ionization [M+H] + for identification [21,37,38] [39], giving characteristic ions for the compounds. The data obtained from the trypanocidal activity suggest that the isolated compounds need a lower concentration to inhibit T. cruzi than the crude extracts.…”

Section: Discussionmentioning

confidence: 96%

“…Following the previously mentioned processes and according to the results of the active fractions identified by disc-diffusion and bioautographic methods, we decided to carry out the detection of parthenin by eluting the compounds using TLC with a mixture of chloroform:acetone (3:1). When sprayed with the vanillin reagent with sulfuric acid, they showed a violet-blue band with an Rf value of around 0.6 after heating at 70 °C for 5 min, which is something characteristic of parthenin [21]. Subsequently, parthenin was recovered from the active fractions PTLC with the help of a vanillin reagent.…”

Section: Discussionmentioning

confidence: 99%

Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth

Hernández-Marín,

Castro-Rios,

Chavez-Montes

et al. 2023

Preprint

View full text Add to dashboard Cite

Parthenium incanum (Mariola) is used in Mexico to treat stomach and liver disorders. Reports have shown that the genus Parthenium contains biologically active metabolites. This work aimed to isolate and identify biologically active compounds with trypanocidal activity. The extracts were obtained through static maceration and extraction with successive reflux using different solvents. After that, they were fractionated with column chromatography, and the obtained fractions were compared by TLC; similar fractions were pooled and concentrated. A bioguided assay was performed with S. aureus ATCC 25923, using agar diffusion techniques and bioautography to determine preliminary biological activity. Active fractions were obtained and purified from a preparative TLC plate. The obtained compounds were identified as parthenin and coronopolin by TLC, FT-IR, and HPLC-MS. Finally, they were evaluated against the Ninoa strain of Trypanosoma cruzi, obtaining an IC50 of up to 45±2.5 ppm. It was concluded that P. incanum contains compounds with trypanocidal activity.

show abstract

“…Parthenin can be extracted from its natural source, P . hysterophorus , using the powdered plant material; fractioned by preparative high-performance liquid chromatography with UV detection [ 38 , 39 , 40 ]; or produced synthetically via several methods, reviewed in detail by Barbero and Prandi [ 11 ]. The first total synthesis of racemic parthenin was performed by Kok et al [ 23 ].…”

Section: Structure Of Partheninmentioning

confidence: 99%

Parthenin—A Sesquiterpene Lactone with Multifaceted Biological Activities: Insights and Prospects

Kaur

Jandrotia

et al. 2021

Molecules

View full text Add to dashboard Cite

Parthenin, a sesquiterpene lactone of pseudoguaianolide type, is the representative secondary metabolite of the tropical weed Parthenium hysterophorus (Asteraceae). It accounts for a multitude of biological activities, including toxicity, allergenicity, allelopathy, and pharmacological aspects of the plant. Thus far, parthenin and its derivatives have been tested for chemotherapeutic abilities, medicinal properties, and herbicidal/pesticidal activities. However, due to the lack of toxicity–bioactivity relationship studies, the versatile properties of parthenin are relatively less utilised. The possibility of exploiting parthenin in different scientific fields (e.g., chemistry, medicine, and agriculture) makes it a subject of analytical discussion. The present review highlights the multifaceted uses of parthenin, on-going research, constraints in the practical applicability, and the possible workarounds for its successful utilisation. The main aim of this comprehensive discussion is to bring parthenin to the attention of researchers, pharmacologists, natural product chemists, and chemical biologists and to open the door for its multidimensional applications.

show abstract

Determination of parthenin in Parthenium hysterophorus L. by means of HPLC-UV: Method development and validation

Cited by 9 publications

References 6 publications

Quantification of santonin in eight species of Artemisia from Kazakhstan by means of HPLC-UV: Method development and validation

Quantification of santonin in eight species of Artemisia from Kazakhstan by means of HPLC-UV: Method development and validation

Trypanocidal Activity of Compounds Isolated From Parthenium incanum Kunth

Parthenin—A Sesquiterpene Lactone with Multifaceted Biological Activities: Insights and Prospects

Contact Info

Product

Resources

About