Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Chen, Cuicui; Guo, Xiaoxiao; Hang, Liang Wen; Niu, Bo; Li, Jiexing; Zhong, Shuhai; Liu, Xipan; Li, Laiqing

doi:10.1002/bab.1758

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Canine parvoviral enteritis is a highly contagious and fatal disease of dogs and many other carnivores, which results in severe gastroenteritis and myocarditis (Chen et al 2019). The disease is caused by canine parvovirus (CPV) which is one of the most important causes of mortality in young dogs and that has sustained pandemic circulation in dogs for more than 40 years (Voorhees et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report

et al. 2020

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This report describes the clinical presentation, pathology and molecular diagnosis of canine parvovirus infection in male Boerboel and female Alsatian puppies. The history of the dogs was considered, examined clinically for vital parameters, haemogram changes and faeces screened for parasites and canine parvovirus faecal antigen. Tissue samples were taken at necropsy for confirmatory diagnosis using histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. There was a severe regenerative anaemia, leucopenia and lymphopaenia. The positive antigen faecal test and pathological findings of haemorrhagic enteritis suggested canine parvoviral enteritis disease. Polymerase chain reaction and sequence analysis confirmed canine parvovirus-2a as the aetiology of the disease. Informed management is important to avoid complications resulting from secondary to severe dehydration, hypovolemia from marked gastrointestinal fluid and protein loss and sepsis from bacterial translocation and leukopenia.

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Section: Introductionmentioning

confidence: 99%

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report

et al. 2020

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“…At present, the regular methods for the detection of canine virus antigen are colloidal gold immunochromatography and ELISA in clinical, and there is no product specifically for the quantitative detection of virus antigens in vaccines [13–15]. Colloidal gold immunochromatography is one of the fastest and most commonly used qualitative detection method, whose disadvantage is the inability to perform quantitative detection [16]. Moreover, the ELISA method could realize quantitative detection, but its detected sensitivity is not high enough to cover the sample with low concentration CPV‐2‐Ag, and dynamic range is not wide enough to cover all the vaccines.…”

Section: Discussionmentioning

confidence: 99%

Determination of parvovirus antigen in the vaccine using time‐resolved fluorescence immunoassay

Chen¹,

Hang²,

Hu³

et al. 2020

Biotech and App Biochem

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As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection of CPV-2 in vaccine. Therefore, this study aims to prepare an accurate method to determine the CPV-2 antigen (CPV-2-Ag) in vaccine. Here, a sandwich time-resolved fluorescence immunoassay (TRFIA) was established and optimized. Anti-CPV-2 antibodies were immobilized on 96-well plates to capture CPV-2-Ag, and then bound together with the detection antibodies labeled with Europium(III) (Eu 3+ ) chelates; finally, time-resolved fluorometry was employed to measure the fluorescence intensity. Vaccination was performed to evaluate the relationship between CPV-2-Ag concentration and antibody titer. The sensitivity is 1.15 mEU/mL (LogY = 1.524 + 0.8667 × LogX, R 2 = 0.9933), and the average recovery is among 91.00% to 106.39% without cross-reactions with the other canine viral antigen. The correlation between ELISA assay and this method is up to 0.9861. And, there is high correlation between the CPV-2-Ag concentration and antibody titers (R 2 = 0.9234). This immunoassay established has high sensitivity, accuracy, and specificity, which indicate that this method could be suitable for quantitative detection of CPV-2-Ag in vaccine evaluation.

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“…Acridinium ester has been successfully used to perform ultrasensitive immunoassays of various proteins and antibodies. tumor markers (a-fetoprotein), thyroid stimulating hormone (TSH) and immunoglobulins (Ma et al, 2017;Min et al, 2018;Chen et al, 2019). Acridinium ester is also able to successfully label strands of DNA to produce DNA probes to emit CL (Komori et al, 2019).…”

Section: Acridinium Estersmentioning

confidence: 99%

Ultrasound-Enhanced Chemiluminescence for Bioimaging

Dhamecha

Gonsalves

et al. 2020

Front. Bioeng. Biotechnol.

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Tissue imaging has emerged as an important aspect of theragnosis. It is essential not only to evaluate the degree of the disease and thus provide appropriate treatments, but also to monitor the delivery of administered drugs and the subsequent recovery of target tissues. Several techniques including magnetic resonance imaging (MRI), computational tomography (CT), acoustic tomography (AT), biofluorescence (BF) and chemiluminescence (CL), have been developed to reconstruct three-dimensional images of tissues. While imaging has been achieved with adequate spatial resolution for shallow depths, challenges still remain for imaging deep tissues. Energy loss is usually observed when using a magnetic field or traditional ultrasound (US), which leads to a need for more powerful energy input. This may subsequently result in tissue damage. CT requires exposure to radiation and a high dose of contrast agent to be administered for imaging. The BF technique, meanwhile, is affected by strong scattering of light and autofluorescence of tissues. The CL is a more selective and sensitive method as stable luminophores are produced from physiochemical reactions, e.g. with reactive oxygen species. Development of near infrared-emitting luminophores also bring potential for application of CL in deep tissues and whole animal studies. However, traditional CL imaging requires an enhancer to increase the intensity of low-level light emissions, while reducing the scattering of emitted light through turbid tissue environment. There has been interest in the use of focused ultrasound (FUS), which can allow acoustic waves to propagate within tissues and modulate chemiluminescence signals. While light scattering is decreased, the spatial resolution is increased with the assistance of US. In this review, chemiluminescence detection in deep tissues with assistance of FUS will be highlighted to discuss its potential in deep tissue imaging.

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Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Cited by 12 publications

References 21 publications

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report

Determination of parvovirus antigen in the vaccine using time‐resolved fluorescence immunoassay

Ultrasound-Enhanced Chemiluminescence for Bioimaging

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