Determination of patulin in apple juice produced in Isparta, Turkey by HPLC with diode array detection

Aktaş, Ahmet; Yilmazer, Mustafa; Demirci, Seda

doi:10.38212/2224-6614.2638

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…With this method, 5-HMF interference can occur in determining PAT in apple juices and derived products. Moreover, 5-HMF is present at levels two or three times higher than PAT, which can cause a severe problem in determining PAT in biological samples [50]. The identity of PAT in the two positive samples was confirmed by UHPLC-MS/MS, as described in the Supplementary Information.…”

Section: Confirmation With Uhplc-ms/msmentioning

confidence: 84%

Dispersive solid phase extraction using a hydrophilic molecularly imprinted polymer for the selective extraction of patulin in apple juice samples

Cavaliere,

Cerrato,

Laganà

et al. 2023

Microchim Acta

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A molecularly imprinted polymer with a specific selectivity for patulin was successfully synthesized. The molecularly imprinted material was prepared using the two functional monomers dopamine and melamine and formaldehyde as the cross-linker. The resulting material possessed a large number of hydrophilic groups, such as hydroxyls, imino groups, and ether linkages. For the first time, uric acid was used as a dummy template for its structural similarity to patulin. Comprehensive characterization and detailed studies of the adsorption process were carried out via adsorption isotherms, while the rate-limiting steps were investigated using adsorption kinetics. Separation, determination, and quantification of patulin were achieved by ultra-high performance liquid chromatography coupled with both photodiode array detection and tandem mass spectrometry. The latter was applied to patulin confirmation in the analysis of real samples. The methodology was validated in 20 apple juice samples. The results showed that the developed hydrophilic molecularly imprinted polymer had high selectivity and specific adsorption towards patulin, with mean recoveries ranging between 85 and 90% and a relative standard deviation lower than 15%. The developed molecularly imprinted polymer exhibited good linearity in the range 1–100 ng mL−1 with coefficient of determination (R2) > 0.99. The limit of detection was 0.5 ng mL−1, and the limit of quantification was 1 ng g−1. The developed method showed a good purification capacity for apple juices due to its hydrophilic nature and the polar interactions established with the target analyte. Graphical abstract

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Section: Confirmation With Uhplc-ms/msmentioning

confidence: 84%

Dispersive solid phase extraction using a hydrophilic molecularly imprinted polymer for the selective extraction of patulin in apple juice samples

Cavaliere,

Cerrato,

Laganà

et al. 2023

Microchim Acta

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Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods

Reddy

Abbas

Abel

et al. 2010

Toxins

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Abstract:Mycotoxins are toxic secondary metabolites produced under special conditions of moisture and temperature by aerobic, microscopic fungi that can colonize a variety of foods from before harvest up to consumer use. Contamination of beverages, including that of apple juice by patulin and coffee, beer and wine by ochratoxin A has gained worldwide interest in recent years with the revelation of the effect of these toxins on human health. Patulin and ochratoxin A, which are produced by species of Penicillium, Byssochlamys and Aspergillus, pose the greatest threat to human health and are subject to government regulation in juices and beverages worldwide. This review provides an overview of the prevalence of these mycotoxins in beverages and control methods which help in establishing and carrying out proper management strategies. A detailed investigation on OPEN ACCESSToxins 2010, 2 230 contamination of juices and beverages by toxic compounds helps to provide safe products for consumption and export, and they allow us to prioritize future research efforts.

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Determination of patulin in apple juice produced in Isparta, Turkey by HPLC with diode array detection

Cited by 2 publications

References 10 publications

Dispersive solid phase extraction using a hydrophilic molecularly imprinted polymer for the selective extraction of patulin in apple juice samples

Dispersive solid phase extraction using a hydrophilic molecularly imprinted polymer for the selective extraction of patulin in apple juice samples

Mycotoxin Contamination of Beverages: Occurrence of Patulin in Apple Juice and Ochratoxin A in Coffee, Beer and Wine and Their Control Methods

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