Determination of penicillins by desulphurization with lead and edta titration

Hassan, Saad S. M.; Zaki, M. T. M.; Eldesouki, M. H.

doi:10.1016/0039-9140(79)80223-4

Cited by 13 publications

(3 citation statements)

References 8 publications

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“…The use of an adequate ratio of BSA to GLA is critical for successful entrapment of enzymes 18, 19. To determine the best ratio of these reagents in this study, the effect of BSA concentration on the potentiometric response of penicillin was investigated by keeping the GLA concentration constant, while varying the BSA concentration.…”

Section: Resultsmentioning

confidence: 99%

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Comparison of Single Layer and Bilayer Biosensors Based on Crosslinking of Penicillinase for Potentiometric Detection of Penicillin in Milk and Antibiotics

Ismail

Adeloju

2015

Electroanalysis

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The use of bilayers for fabrication of biosensors is advantageous for increasing enzyme loading. Substantial improvement in sensitivity is often achieved through immobilisation of the enzyme in both layers. In particular, the use of cross linking agents such as glutaraldehyde (GLA), bovine serum albumin (BSA) and polyvinyl alcohol (PVA) are beneficial for enhancing enzyme stability and, hence, for fabricating stable biosensors. The successful fabrication of a single layer BSA‐GLA‐P’nase biosensor for potentiometric detection of penicillin is described. Subsequently, the three crosslinking agents were employed with two polymers, polypyrrole (PPy) and polytyramine (PTy), together with penicillinase (P’nase) for fabrication of PPy‐NO3‐P’nase/BSA‐PVA‐P’nase and PTy‐NO3‐P’nase/BSA‐GLA‐P’nase bilayer biosensors. The analytical performances of the bilayer biosensors were then compared with the single layer BSA‐GLA‐P’nase biosensor for the determination of penicillin in milk and amoxycillin tablets. While the determination of penicillin in milk was somewhat problematic, its determination in amoxicillin tablets proved to be successful, with recoveries of 102±15 % obtained with the PPy‐NO3‐P’nase/BSA‐PVA‐P’nase biosensor, 100±19 % with PTy‐NO3‐P’nase/BSA‐GLA‐P’nase biosensor and 103±5 % with BSA‐GLA‐P’nase biosensor. Notably, the results of the latter agreed favourably with those obtained through a reference titrimetric method.

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Section: Resultsmentioning

confidence: 99%

“…The colour of the chloroform layer changed from colourless to brown/deep red to colourless again. The end point was taken as the first permanent decolourisation of the chloroform layer 18. This procedure was repeated for all three tablets.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Single Layer and Bilayer Biosensors Based on Crosslinking of Penicillinase for Potentiometric Detection of Penicillin in Milk and Antibiotics

Ismail

Adeloju

2015

Electroanalysis

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“…[4] According to State Pharmacopoeia of Ukraine and European Pharmacopoeia,penicillin quantitative determination is performed by high-performance liquid chromatography. [5,6] The following quantitative procedures of penicillin determination are described: Using potentiometry titration and ionometry, [7] spectrophotometry, [8][9][10] extraction photometry, [11] voltammetry [12] and polarography, [13] micelle electrokinetic capillary [14] and paper chromatography, [15,16] and chemiluminescence [17] and kinetic analysis methods. [18] A new procedure for the quantitative determination of Azl sodium in the Securopen ® preparation by the method of back iodometric titration using potassium hydrogenperoxomonosulfate (KHSO 5 ) as an analytical reagent was developed.…”

Section: Research Articlementioning

confidence: 99%

Untitled

Karpova¹

2018

AJP

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Objective: Kinetics and stoichiometry S-oxidation reaction of sodium Azlocillin (Azl) by means of potassium hydrogenperoxomonosulfate in aqueous solutions using iodometric titration were studied. Materials and Methods: Securopen ®-powder Azl sodium in flacons for preparation of solution for injections (Azl 1.0 g; 5.0 g) was used for analysis. A new iodometric method for quantitative determination of sodium Azl in Securopen ® preparation using potassium hydrogenperoxomonosulfate (KHSO 5) as analytical reagent was proposed. Peroxomonosulfate acid as triple potassium salt 2КНSO 5 •КНSO 4 •K 2 SO 4 (Oxone ®) of "extra pure" qualification was used as oxidant. Results: At pH 2-4 for 1 mole of penicillin, 1 mole of KHSO 5 is consumed; the quantitative interaction is achieved within a time of more than 1 minute (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. Relative standard deviation = (0.8 −2.8) %, δ = (+0.2 …−0.31) %. It can be seen that Azl can be determined successively with good accuracy and reproducibility. Conclusion: The new procedure was developed and ability of quantitative determination of penicillin in pharmaceutical preparation Securopen ® by iodometric method using potassium hydrogenperoxomonosulfate (KHSO 5) as analytical reagent was shown.

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Microdetermination of divalent sulphur in aliphatic compounds by visual titrimetry, potentiometry, and atomic absorption spectrometry

Hassan

Eldesouki

1979

Mikrochim Acta

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Determination of penicillins by desulphurization with lead and edta titration

Cited by 13 publications

References 8 publications

Comparison of Single Layer and Bilayer Biosensors Based on Crosslinking of Penicillinase for Potentiometric Detection of Penicillin in Milk and Antibiotics

Comparison of Single Layer and Bilayer Biosensors Based on Crosslinking of Penicillinase for Potentiometric Detection of Penicillin in Milk and Antibiotics

Untitled

Microdetermination of divalent sulphur in aliphatic compounds by visual titrimetry, potentiometry, and atomic absorption spectrometry

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