Determination of pentoxifylline in serum by high-performance thin-layer chromatography

Degradation studies of pentoxifylline: Isolation and characterization of a novel gem-dihydroperoxide derivative as major oxidative degradation product

“…These include SFC method [5], HPTLC method [6], HPLC reverse phase methods using various particle size columns and buffers [7][8][9][10]. The drug was also studied extensively for pharmacokinetics and metabolism in animals and human subjects leading to development of a number of HPLC methods using UV and mass detection [11][12][13].…”

Study of the solid phase extraction of pentoxifylline and its major metabolite as a basis of their rapid low concentration gas chromatographic determination in serum

“…The drug is extensively eliminated (t,,2 0.4-0.8 h; Smith et al, 1986) and one of its major circulating metabolites in plasma is a secondary alcohol metabolite 1-(5-hydroxyhexy1)-3,7-dimethylxanthine (OH-PETX; 2). Several bioanalytical methods have been developed for the determination of PETX in different physiological fluids, including thin layer chromatography (TLC) (Bauerova et al, 1991) , high performance liquid chromatography (HPLC) (Von Stetten et al, 1985;Luke and Rocci, 1986;Garnier-Moiroux et aE., 1987;Grasella and Rocci, 1987;Musch et al, 1991) and gasliquid chromatography (GLC) (Burrows and Jolley , 1985;Burrows, 1987). The limit of determination was in the range of nanograms per millilitre in GLC methods and approximately one order higher in HPLC and TLC.…”

“…The solid phase extraction method was also used in combination with TLC. Bauerova et al (1991) extracted PETX and its metabolites on C,8 silica sorbents before their separation on high performance thin layer chromatographic (HPTLC) plates with a preconcentration zone.…”

Development of a HPTLC method for in-process purity testing of pentoxifylline

“…In addition, chromatographic techniques such as HPLC [10 -15], SFC [16], GC [17,18] and HPTLC [19] have been widely employed for the determination of pentoxifylline and its metabolites in biological fluids and tissues. Also, TLC and HPTLC have been used for the determination of pentoxifylline and mebendazole as residues on the surfaces of pharmaceutical equipment [20].…”