Determination of Periodontal Ligament Cell Viability in Long Shelf-Life Milk

Abstract

The purpose of this study was to determine the ability of long shelf-life milk to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability on avulsed teeth. PDL cells were plated onto 24-well culture plates and allowed to attach for 24 h. Minimal Essential Medium was replaced with regular pasteurized milk (refrigerated milk), long shelf-life milk (Parmalat), or Save-A-Tooth. Tap water served as the negative control, and Minimal Essential Medium served as the positive control. The tissue culture plates were incubated at 37 degrees C for 1, 2, 4, or 8 h. Cell viability was determined using a cell proliferation assay (CellTiter 96 AQ Assay) and absorbance read at 490 nm. ANOVA indicated that all media performed significantly better than tap water at all time periods. At 8 h, PDL cell viability in regular pasteurized milk and long shelf-life milk were significantly greater than in Save-A-Tooth (p < or = 0.001). There was no significant difference between regular pasteurized milk and long shelf-life milk at any time period. These results suggest that long shelf-life milk, which has the advantage of not requiring refrigeration, is as effective a storage medium for avulsed teeth as regular pasteurized milk and more effective than Save-A-Tooth.

Aqueous Extract of Psidium cattleianum as Intracanal Medication: An In Vitro Study of Cell Viability

“…Thus, the extract appears to have neutralized the detrimental effect that distillated water performed. The water is a hypotonic medium that can lead to rapid lysis of the cell membrane [25].…”

Egg White-Milk Mixture as a Novel Biological Storage Media for Avulsed teeth Periodontal Ligament Cells: An In-Vitro Study

“…These contents are needed by cells to prevent starvation, dehydration in addition to proper pH and osmolality. As well as being free of bacteria that decrease infection possibility [32,43]. Bloml., et al stated that milk is an excellent storing solution for 6 hours when immediate replantation is not possible [22].…”

Medios de almacenamiento y transporte para dientes avulsionados

“…• Agua: La osmolalidad del agua varía de 3 -16 mOsm/Kg y su pH es de 7,41 4,20 es el medio de transporte menos adecuado pues al ser hipotónica desencadena la lisis celular, adicionalmente e l a l m a c e n a m i e n t o e n a g u a más de veinte minutos provoca grandes reabsorciones radiculares 14,20,23,25,27,29 • Saliva: Por tener una osmolalidad de 60 -80 mOsm/Kg y pH de 6,76 -7,35 [12][13][14][15][16]19,20 como por contener bacterias no es muy idónea. No obstante, los estudios demuestran que si el diente se pone en el vestíbulo bucal, los fibroblastos pueden mantenerse vitales unas dos horas.…”

“…26,29 • L e c h e : E n 1 9 8 1 A n d r e a s e n (Dinamarca) y Blomlof, Lindskog y Hammarstrom (Suecia) fueron los primeros en reportar sobre la viabilidad de la leche fresca descremada pasteurizada como medio para conservar un diente avulsionado. La leche si no fuera por el contenido de lípidos 3,20,23,25 sería un excepcional medio; no obstante, en las condiciones en que se produce un traumatismo dentario es el mejor medio de transporte porque es fácil de conseguir y porque es un líquido estéril debido a la pasteurización…”