Determination of permeation pathways of hydrophilic or hydrophobic dyes through the mammary papilla

Kurtz, Samantha L.; Lawson, Louise B.

doi:10.1016/j.ijpharm.2018.04.033

Cited by 12 publications

(27 citation statements)

References 24 publications

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“…The 70% and 80% water nanoemulsions formulations deposited significantly more NR only in the uppermost 1 mm of tissue as compared to the control solution, but did so at both the 6- and 48-hour time point. As tissue depth increased, the amount of dye deposited in the tissue decreased for each treatment, which is consistent with previous reports 9,28 .…”

Section: Resultssupporting

confidence: 92%

“…These epithelial cells represent only 8% of the total cellular makeup of the mammary gland 7 , but are the cell population that most commonly malignantly transforms, accounting for 95% of breast cancer cases 8 . We and others have demonstrated that diffusion through and retention within the mammary canals varies depending on a molecule’s and drug carrier’s physicochemical properties 9–11 . Specifically, lipophilic small molecules preferentially diffuse through the conduits, capitalizing on the ductal orifices as an entry point and transport pathway.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, lipophilic small molecules preferentially diffuse through the conduits, capitalizing on the ductal orifices as an entry point and transport pathway. Furthermore, drug carriers with diameters of at least 5 nm injected intraductally remain concentrated within the mammary ducts 9,12 , thereby limiting exposure within the surrounding breast stroma and distant extraneous organs. Enhanced targeting of the ductal orifices and passive diffusion through the canals may therefore be accomplished through design of tailored drug delivery vehicles.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the targeting capabilities within the ductal channels of emulsion-based formulations remain unknown. Our own previous investigation of transpapillary delivery, in which we used qualitative and quantitative assessments to analyze permeation at both a micro and macro scale, indicates that permeation occurs via two distinct pathways, transepidermal and transductal 9 . Herein, we sought to use similar techniques to elucidate the role of emulsion-encapsulation on transport via the mammary papillae.…”

Section: Introductionmentioning

confidence: 99%

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Nanoemulsions Enhance in vitro Transpapillary Diffusion of Model Fluorescent Dye Nile Red

Kurtz

Lawson

2019

Sci Rep

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While the feasibility of transpapillary drug delivery has previously been established, localized transport via the mammary ducts may be improved with tailored drug delivery formulations. The objective of this study was to investigate the impact of nanoemulsion encapsulation on transpapillary delivery in vitro . Nanoemulsion formulations composed of isopropyl myristate and Tween 80 encapsulating a fluorescent dye were applied topically on porcine nipples using a Franz diffusion cell. A combination of dye extraction and fluorescence image analysis was used to quantify the total amount of dye retained within the nipple and to characterize the penetration routes. After diffusion for 6 hours, the amount of dye deposited in the nipple was proportional to the formulation’s water concentration. The 90% water formulation deposited significantly more dye via both the stratum corneum and mammary ducts, while the 80% and 70% water formulations moderately increased ductal penetration, but minimally altered stratum corneum penetration as compared to the control solution. Similar trends were found after diffusion for 48 hours; however, the overall impact was diminished, likely due to the nanoemulsion’s topical instability. This study indicates that drug delivery vehicles, nanoemulsions specifically, enhance delivery of encapsulated molecules via the stratum corneum and mammary ducts in a formulation-dependent basis.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanoemulsions Enhance in vitro Transpapillary Diffusion of Model Fluorescent Dye Nile Red

Kurtz

Lawson

2019

Sci Rep

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“…those that do not have cells, but have a compound (ODb). The IC was calculated with the following formula [50]: % IC = 100 − (ODt − ODb)/(ODc − ODb) × 10…”

Section: Methodsmentioning

confidence: 99%

Apoptosis Induction of Agave lechuguilla Torrey Extract on Human Lung Adenocarcinoma Cells (SK-LU-1)

Anguiano-Sevilla

Lugo‐Cervantes

Ordaz-Pichardo

et al. 2018

IJMS

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In this study, an ethanol extract of Agave lechuguilla was evaluated against six carcinogenic cell lines (HCT-15, MCF-7, PC-3, U-251, SK-LU-1 and K-562) with an inhibition of 75.7 ± 2.3% against the SK-LU-1 line. Based on the previous result, the extract was hydrolyzed and fractionated, to which the IC50 was determined; the cell line was more sensitive to the fractionated extract with an IC50 6.96 ± 0.15 µg/mL. Characterization by mass spectrometry showed the presence of kaempferol, quercetin and a flavonoid dimer formed by afzelechin-4β-8-quercetin, according to the generated fragmentation pattern. The fractionated extract presented cell death by apoptosis with 39.8% at 24 h. Molecular docking was performed with the molecules found to try to describe cell death by apoptosis through death receptors such as FasCD95, TNF-R1, DR4/5 and blocking signaling on the EGFR and K-Ras MAPK/ERK pathway, as well as through the intrinsic pathway activating tBID, which promotes the amplification of the apoptotic signal due to the activation of caspase-3, and consequently caspase-7. In addition to the activation of the IIb complex associated with cell death due to necroptosis.

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Therapeutische Besonderheiten bei Erkrankungen der Mamillenhaut

2022

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ZusammenfassungDie Mamillenregion ist durch besondere anatomische Verhältnisse charakterisiert und lässt sich aus dermatologischer Perspektive in Brusthaut, Haut des Brustwarzenhofes (Areola) und Haut der Brustwarze (Papilla mammae) unterteilen. Im klinischen Zusammenhang sind die Brustwarzen häufig während der Stillzeit durch mechanische Beanspruchung, Milieuänderung mit Mazeration durch den Milchfluss sowie durch mikrobielle Erreger alteriert. Zudem besteht hier die Gefahr der Entwicklung einer Mastitis puerperalis. Außerhalb der Schwangerschaft und Stillzeit finden sich gelegentlich an der Mamillenhaut Ekzemerkrankungen, häufig bei atopischer Disposition (atopisches Mamillenekzem) oder als irritatives Kontaktekzem („joggers nipple“). Seltener werden allergische Kontaktekzeme auf Konservierungsstoffe von Topika oder Metallen (Piercings) beobachtet. Auch im Rahmen einer Skabiesinfestation wird eine Beteiligung der Mamillen, insbesondere bei Frauen, regelmäßig beobachtet. Von großer klinischer Bedeutung sind seltene, präinvasive Läsionen eines Mammakarzinoms oder der Morbus Paget der Mamille vom extramammären Typ. Durch die besonderen anatomischen Gegebenheiten ist es naheliegend, dass bei der Anwendung von Topika sich auch spezifische Penetrationsbedingungen ableiten. Experimentelle Untersuchungen an Humanhaut ex vivo legen nahe, dass in Abhängigkeit von der Molmasse und der Löslichkeit des Arzneistoffs sowie des eingesetzten Vehikelsystems eine deutliche Zunahme der kutanen Bioverfügbarkeit, insbesondere an der Brustwarze selbst durch den transpapillären Diffusionsweg, auftreten kann. Dies sollte insbesondere bei der topischen Anwendung von Arzneistoffen mit bekanntem dosisabhängigem Nebenwirkungspotenzial (z. B. Glukokortikoiden) beachtet werden. Allerdings fehlt dafür bisher eine klinische Evidenz.

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Determination of permeation pathways of hydrophilic or hydrophobic dyes through the mammary papilla

Cited by 12 publications

References 24 publications

Nanoemulsions Enhance in vitro Transpapillary Diffusion of Model Fluorescent Dye Nile Red

Nanoemulsions Enhance in vitro Transpapillary Diffusion of Model Fluorescent Dye Nile Red

Apoptosis Induction of Agave lechuguilla Torrey Extract on Human Lung Adenocarcinoma Cells (SK-LU-1)

Therapeutische Besonderheiten bei Erkrankungen der Mamillenhaut

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