DETERMINATION OF PESTICIDE RESIDUES IN BOVINE MILK USING A MODIFIED QuEChERS METHOD AND GC-MS/MS

Bandeira, Danieli Daiani; Munaretto, Juliana S.; Rizzetti, Tiele Medianeira; Ferronato, Giovana; Prestes, Osmar D.; Martins, Manoel Leonardo; Zanella, Renato; Adaime, Martha B.

doi:10.5935/0100-4042.20140145

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…Consequently, the TIC of the instrumental runs for each of the mobile phases resulted in chromatographic peak heights (ATCPH), and areas (ATCPAs) as presented in Table 3. Then again, the addition of organic solvent into aqueous mobile phase could provide the optimum condition of logP , which contributes to the attainment of good condition for the multi-pesticide residues analysis in food samples using LC-MS/MS instrument as revealed [41]. For this reason, optimization was carried out by serial addition of ACN into the aqueous mobile phase (0.1% FA milli-Q-water).…”

Section: Sample Treatment and Methodologymentioning

confidence: 99%

Mobile Phase Selection by Optimization for the Determination of Multiple Pesticides Using Liquid Chromatography-Tandem Mass Spectrometry

Lawal¹,

Abdulra’uf²

2022

Biodegradation Technology of Organic and Inorganic Pollutants

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The selection of the best mobile phase setup is one of the most important factors to be considered prior to quantitative instrumentation of multiple pesticides. Usually, mobile phases comprises of water (A) and an organic solvent (B) are the setup used in liquid chromatography instruments for the analysis of pesticide residues in various samples. Unfortunately, most of the analyses are being carried out without optimization and selection of the best mobile phase setup to improve the sensitivity of the instrument. For that reason, the comparative analysis of the reportedly used mobile phases and some few suggested ones was carried out on the multi-pesticide mixture of 0.1 mg/kg (100 μg/kg) standard solutions and quantified with liquid chromatography–tandem mass spectrometry (LC–MS/MS) instrument. Consequently, the best mobile phases setup that resulted in the sum of average total chromatographic peak areas (ATCPAs) and average total chromatographic peak heights (ATCPH) for the total ion chromatography (TIC) scans as an index that correspond to the concentration levels was selected [0.1% formic acid in H2O (A) and 0.1% formic acid in acetonitrile (ACN) (B)]. And further optimization was successfully carried out on the selected mobile phase-A and the resulted setup [1% ACN and 0.1% formic acid in Milli-Q-water (mobile phase A) coupled with 0.1% formic acid in ACN (mobile phase-B)] improved the instrumental sensitivity on the targeted analytes. Thus, this justify the potential benefits of optimizing setup of the mobile phases prior to LC–MS/MS instrumentation of multi-pesticide analytes.

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Section: Sample Treatment and Methodologymentioning

confidence: 99%

Mobile Phase Selection by Optimization for the Determination of Multiple Pesticides Using Liquid Chromatography-Tandem Mass Spectrometry

Lawal¹,

Abdulra’uf²

2022

Biodegradation Technology of Organic and Inorganic Pollutants

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“…Although it is characterized by lower sensitivity than GC-MS and LC-MS and normally is not used for the analysis of complex samples, HPLC combined with ultraviolet (UV) and/or Diode Array Detector (DAD) is used for determination of organophosphorus and triazines in different matrices (Sanchez-Ortega et al, 2005). The analysis of pesticide residues necessarily involves pretreatment of samples, and for this purpose some of the following techniques are used: Liquid-Liquid Extraction (LLE) (Jeannot et al, 2009), Solid Phase Extraction (SPE) (Topuz et al, 2005), Liquid-Liquid Microextraction (LLME) (Cunha et al, 2009), Solid Phase Microextraction (SPME) (Hercegová and Mőder, 2011), Matrix Solid-Phase Dispersion (MSPD) (Chua et al, 2005) and recently used, a quick, easy, cheap, effective, rugged and safe (QuEChERS) method (Zanella et al, 2013). In a previous study HPLC method was developed for determination of selected pesticides using DAD (Velkoska-Markovska and Petanovska-Ilievska, 2013;Velkoska-Markovska et al, 2017a, 2017b.…”

Section: Introductionmentioning

confidence: 99%

Determination of Some Pesticide Residues in Apple Juice by High-Performance Liquid Chromatography

Velkoska-Markovska

Petanovska‐Ilievska

Aleksandar

2019

JAFES

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A new, simple and reliable reversed-phase high-performance liquid chromatography (RP-HPLC) method for determination of 2,4-D ((2,4-dichlorophenoxy)acetic acid), atrazine, malathion, fenitrothion and parathion residues in apple juices has been developed and validated. Successful separation and quantitative determination of analytes were performed on Purospher STAR RP-8e (30 x 4 mm, 3 µm) analytical column, with mobile phase consisted of acetonitrile/water (45/55, V/V), flow rate of 1 mL/min, constant column temperature at 25 0 C and UV detection at 220 nm and 270 nm. A solid-phase extraction (SPE) was used for concentration and clean-up of analytes. Specificity, selectivity, linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were tested for the method validation according to European Commission guidelines for pesticide residue analytical methods, and all performance characteristics were found within acceptance criteria. Calibration curves were linear within the range less than 50 % of the maximum residue limit (MRL) values and 20 % above for all investigated pesticides (R 2 > 0.95). Under the stipulated conditions, values for relative standard deviations (RSD) of retention times and peak areas were 0.12 % -0.28 % and 0.08 % -8.50 %, respectively. The obtained values for recoveries ranged from 93.65 % -118.62 %, with RSD ≤ 5.64 %. This method was successfully applied for determination of investigated pesticide residues in apple juice samples, which were taken from Macedonian markets. The run time of assay obtained from this chromatography condition was about 4 min.

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DETERMINATION OF PESTICIDE RESIDUES IN BOVINE MILK USING A MODIFIED QuEChERS METHOD AND GC-MS/MS

Cited by 2 publications

References 31 publications

Mobile Phase Selection by Optimization for the Determination of Multiple Pesticides Using Liquid Chromatography-Tandem Mass Spectrometry

Mobile Phase Selection by Optimization for the Determination of Multiple Pesticides Using Liquid Chromatography-Tandem Mass Spectrometry

Determination of Some Pesticide Residues in Apple Juice by High-Performance Liquid Chromatography

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