Determination of petroleum biodegradation by bacteria isolated from drilling fluid, waste mud pit and crude oil

Özyürek, Sezen Bilen; Bilkay, Işıl Seyis

doi:10.1515/tjb-2017-0087

Cited by 19 publications

(16 citation statements)

References 21 publications

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“…The highest petrol degradation efficiency (69.5% ± 13.44 / 6 days) was observed for IUBP15. While highest petrol removal efficiency reported in the literature is 30% per seven days for Bacillus tequilensis grown in the presence of crude oil (1% v/v) for seven days at 30°C (Ozyurek and Bilkay 2017). In the present study, the lowest petrol degradation rate (41% ± 32.6 / 4 days) was noticed for IUBP1.…”

Section: Discussioncontrasting

confidence: 53%

“…This is parallel with the earliest studies in which petroleum hydrocarbons metabolizing bacteria like Bacillus cereus, Proteus mirabilis, Bacillus subtilis, Enterococcus faecalis, Streptomyces sp. ERI-CPDA-1, Bacillus mojavensis ATHE13 and Bacillus licheniformis ATHE9 have been reported (Balachandran et al 2012;Eskandari et al 2017;Ozyurek and Bilkay 2017).…”

Section: Discussionmentioning

confidence: 99%

“…For initial screening of petrol metabolizing bacteria, minimal salt media (MSM) was used. This medium contained; KH 2 PO 4 , 1 g; K 2 HPO 4, 1 g; NH 4 NO3, 1 g; MgSO 4 , 0.2 g; CaCl 2 , 0.02 g; FeCl 3 (0.5 M), 2 drops per 1000 ml of distilled water, and 100 μl petrol in 100 ml of medium (Ozyurek and Bilkay 2017). Initially, 1 g of soil sample was added to the medium (50 ml) and incubated for one week in shaker adjusted at 50°C and at 250 rpm.…”

mentioning

confidence: 99%

“…The culture was collected after different time intervals and centrifuged (6000 rpm) at 4°C. The pelleted cells were discarded, and an absorbance of the supernatant was measured at 600 nm to assess the color change against a blank (Ozyurek and Bilkay 2017). DCPIP serves as a redox indicator and its decolorization or color change in petrol supplemented medium is a measure of petrol metabolizing capability of bacteria (Marchand et al 2017).…”

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confidence: 99%

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An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Muccee

Ejaz

2019

Polish Journal of Microbiology

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The present study aimed to isolate the high-efficiency petrol metabolizing thermophilic bacteria from petrol contaminated soil samples. Isolation was carried out through enrichment culture, serial dilution and pour plate methods using the petrol supplemented minimal salt media. The isolated bacteria were analyzed to document growth behavior, petrol removal efficiencies, antibiotic resistance profile, and biochemical characteristics. The 16S rRNA based phylogenetic analysis helped to reveal the identity of isolated bacterial species and construct the phylogenetic trees. Total nine bacteria were isolated, out of which three (IUBP2, IUBP3, IUBP5) were identified as Brevibacillus formosus, one (IUBP1) was found similar to Brevibacillus agri, four (IUBP7, IUBP8, IUBP13, and IUBP14) shared homology with Burkholderia lata, and one (IUBP15) with Burkholderia pyrrocinia. All the isolates were fast growing and exhibited considerable petrol degradation potential. The highest petrol removal efficiency (69.5% ± 13.44/6 days) was recorded for the strain IUBP15 at a petrol concentration of 0.1% (v/v). All bacteria studied (100%) were positive for esculinase and phosphatase. Many strains exhibited positive responses for arginine dehydrolase (22%), β-naphthylamidase (11%), β-D-glucosaminide (33%), mannitol (55%), sorbitol (66%) and inulin (88%) fermentation test. While all were sensitive to the antibiotics, some of them were found resistant against chloramphenicol and oxacillin. The remarkable biochemical characteristics and considerable petrol removal potential (40–70%) highlights utilization of the bacteria isolated for petrol bioremediation, mineralization of organophosphates, dairy and food industry, and also as biofertilizers and biocontrol agents.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

Muccee

Ejaz

2019

Polish Journal of Microbiology

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“…All these features of fungi make them unique for industrial usage derivatives (1,3). Although the biodegradation of petroleum hydrocarbons with different bacterial strains, bacterial consortia and fungal strains have been investigated in various studies in Turkey, no similar study has been found on biodegradation and biosorption of petroleum by Aspergillus parasiticus NRRL:3386 (11)(12)(13). In this context, the study aimed to determine and to compare biodegradation and biosorption capacities of petroleum by different amounts and concentrations of live/dead biomasses and culture supernatants of A. parasiticus NRRL:3386 with gravimetric, Gas Chromatography/Mass Spectrometry (GC/ MS) and Scanning Electron Microscopy analyses (SEM).…”

Section: Introductionmentioning

confidence: 99%

The Role of Aspergillus parasiticus NRRL:3386 Strain on Petroleum Biodegradation and Biosorption Processes

Özyürek¹,

Avcıoğlu²

2020

Eur J Biol

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Objective: Fungi play an important role in the removal of hazardous organic compounds from the environment with their extracellular multiple enzyme systems. In the bioremediation processes, fungi act as a bioreactor by breaking down or are biosorbent by accumulating organic pollutants. Materials and Methods:The aim of this study was to investigate and to compare the biodegradation and biosorption capacities of different amounts of live and dead biomasses and different concentrations of culture supernatants of Aspergillus parasiticus NRRL:3386 with gravimetric, gas chromatography-mass spectrometry (GC/MS) and scanning electron microscope (SEM) analyses.Results: This study indicated that 1 g of live biomass degraded 80% of petroleum within 4-days of incubation. The cellfree culture supernatant was not as effective as the live biomass in petroleum degradation. The petroleum biosorption was achieved at over 50% by 1 g and 2.5 g; over 70% by 5 g and 7.5 g, and over 80% by 10 g of dead biomasses. The petroleum removal efficiencies of 2 g of live and 10 g of dead biomasses were over 80%. GC/MS analysis demonstrated that C 10 -C 18 n-alkanes (except C 11 and C 13 ) and C 11 , C 13 , C 19 -C 26 n-alkanes were degraded 47-77% and over 80%, respectively. The moststriking result was that C 27 -C 33 n-alkanes were efficiently degraded over 90% in a short incubation period. SEM analysis showed that gaps between fungal hyphae were clear and bright before biosorption of petroleum, whereas gaps between fungal hyphae were closed after biosorption of petroleum. Conclusion:The results clearly pointed out that A. parasiticus will make a significant contribution to advanced mycoremediation studies.

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Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

Mohammed,

Omar Zrary,

Hasan

2023

Biologia

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Petroleum hydrocarbons are a major concern due to their widespread distribution in the environment, and their harmful effects on humans. The use of biodegradation methods to clean soil that has been contaminated by spilt petroleum can be an effective remediation strategy. Therefore, in this study, the presence of indigenous microorganisms in soil contaminated with crude oil was investigated and their ability to degrade petroleum in vitro was studied. Three methods were used to detect crude oil and pure hydrocarbon degradation by bacterial and fungal isolates. Spectrophotometric method, 2,6-Dichlorophenol indophenol (DCPIP) method and hydrocarbon analysis was performed by GC–MS. Furthermore, PCR was carried out to detect the genes AlkB and PMO in the microbial communities. Seventeen bacterial isolates and 5 fungal isolates were recovered from 19 soil culture samples obtained from three different oil-contaminated sites at Taq-taq (TTOPCO) oil field and from a control site without oil contamination. The bacterial samples were identified culturally and morphologically, and the results were validated by genetic analysis. The results of this study revealed that the indigenous bacterial and fungal isolates had a high capacity to degrade crude oil and pure hydrocarbon fractions. Our results provide insight into the great abilities of Bacillus anthracis, Bacillus cereus, Achromobacter sp., Aspergillus lentulus and Rhizopus arrhizus to degrade crude oil and pure hydrocarbon fractions, and these data are supported by the presence of the PMO and AlkB genes in the isolated microorganisms.

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Determination of petroleum biodegradation by bacteria isolated from drilling fluid, waste mud pit and crude oil

Cited by 19 publications

References 21 publications

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

An Investigation of Petrol Metabolizing Bacteria Isolated from Contaminated Soil Samples Collected from Various Fuel Stations

The Role of Aspergillus parasiticus NRRL:3386 Strain on Petroleum Biodegradation and Biosorption Processes

Degradation of crude oil and the pure hydrocarbon fractions by indigenous soil microorganisms

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