Determination of phenolic acids by capillary zone electrophoresis and HPLC

Abstract

Selected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods' characteristics were: linearity (1 -20 mg ml and 0.2 -4 mg ml -1), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability (4.1% and 3.5%), and detection limit (0.2 and 0.02 mg ml -1 ). Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

Biologically active phenolic compounds in buckwheat, oats and winter spelt wheat

“…The raw extracts were stored at −20°C until use. Total phenolic acids contents were tested according to Kvasnička et al (2008). One gram (±0.001 g) of ground sample was weighed into a 100-ml volumetric flask and 25 ml of 0.1 M NaOH added.…”

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

“…The wide use of UV detection was also true for food and food contaminants analyses 85, 177, 190, 196–287. However, increasing also were those exploiting CE‐MS 141, 143–145, 146, 148, 288–303.…”

“…A large number of research articles 141–145, 146, 148, 154, 161, 162, 177, 190, 195–309, 336, 337, 376–384 on CE of food/food contaminants came out during the 2006–2010 coverage. Most of these were on the determination of polyphenolic compounds, the most widely distributed secondary metabolites in plants.…”

“…Several research works, which demonstrated the useful complementary selectivity of CE in relation to HPLC techniques, have been performed, mostly for phenolic compounds 141, 224–226, 229, 248, 283. In the case of MEKC, the unique advantage was the absence of a stationary phase and solubilization of the sample by a micellar phase.…”

Determination of Important Phenolic Compounds in Pakistani Brown Rice Varieties in Controlled, Germinated and Fermented Conditions by High Performance Liquid Chromatography

“…The health benefits and diverse commercial applications of phenolic compounds have prompted the interest of the scientists to increase their concentrations in plant by using different techniques such as genetic engineering [15]. The separation and quantification of phenolic compound has been widely reported previously by several research groups using analytical techniques such as U/HPLC, GC, TLC and CE [16][17][18]. Pakistan is a major producer and exporter of many high value rice varieties.…”