Determination of phenolic acids in fruit juices by isocratic column liquid chromatography

Amakura, Yoshiaki; Okada, Mai; Tsuji, Sumiko; Tonogai, Yasuhide

doi:10.1016/s0021-9673(00)00625-7

Cited by 119 publications

(55 citation statements)

References 8 publications

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“…[21][22][23][24][25] From the scientific literature it is obvious that the most commonly used techniques for the determination of PAs are high-performance liquid chromatography (HPLC) with UV or DAD detection or liquid chromatography coupled with mass spectrometry (LC-MS). [26][27][28][29] Because of the longer sample preparation process for analysis, using gas chromatography with mass spectrometry (GC-MS) in analysis of phenolic compounds is relatively rare, but in comparison with the other methods mentioned, GC-MS offers several advantages, including complete and high-resolution separation, sensitive detection, unambiguous identification and quantitation of a wide range of phenolics (including all isomers) in one chromatographic run. [30][31][32] The aim of our study was to develop a simple and quantitative extraction method of selected PAs to ensure clean extracts in order to obtain a much more sensitive, selective and accurate GC-MS method for identification and quantitation of both free and bound PAs in red wine samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous GC-MS Determination of Free and Bound Phenolic Acids in Slovenian Red Wines and Chemometric Characterization

Ivanović

Razboršek

Kolar

2016

ACSi

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Several phenolic acids (PAs), caffeic, vanillic, syringic, p-coumaric and ferulic acid, found in Slovenian red wines were studied using gas chromatography and mass spectrometry. For isolation of the PAs from wine samples, solid phase extraction using hydrophilic modified styrene -HLB cartridges was used. The bound PAs were extracted after basic hydrolysis and o-coumaric acid was used as the internal standard. The developed method was validated and the linear concentration range for all analytes was from 1 to 100 mg L -1 with correlation coefficients above 0.999. We show that the method is repeatable (RSD<2%), recoveries were above 96%, and LOD and LOQ values were acceptable. In all of the wine samples tested, caffeic and p-coumaric acid were determined to be the predominant PAs (17-72 mg L -1 ), while other compounds were found in lower concentrations. Principal Component Analysis and Cluster Analysis were used to study differences between wines related towards varieties and Slovenian wine regions. The results demonstrate that variety has more influence on PAs content than wine regions in Slovenian red wines.

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Section: Introductionmentioning

confidence: 99%

Simultaneous GC-MS Determination of Free and Bound Phenolic Acids in Slovenian Red Wines and Chemometric Characterization

Ivanović

Razboršek

Kolar

2016

ACSi

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“…Resveratrol and gallic acid are polyphenolic non-flavonoids, widely distributed in the plant kingdom [3], and are present in tea, red wine, fruits, beverages and various medicinal plants [4].…”

Section: Introductionmentioning

confidence: 99%

“…Gallic acid and its derivates are often present in the diet and in wine [15]. This acid phenolic compound is included in the list of existing food additives as natural antioxidants in some countries [4].…”

Section: Introductionmentioning

confidence: 99%

Study of phenolic compounds as natural antioxidants by a fluorescence method

López

Martínez

Valle

et al. 2003

Talanta

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Much work has been carried out in recent years on the beneficial effect of phenolic compounds as natural antioxidants which help to neutralize free radicals. In fact, researchers have focused their attention on the pathological role of free radicals in a variety of diseases, among which the most important are atherosclerosis and cancer. Thus, among the components of the so-called 'Mediterranean Diet', phenolic compounds have received increased attention as epidemiological studies have shown that consumption of foods and beverages rich in phenolics is correlated with reduced incidence of heart disease. In this study, four phenolic compounds: (1) 3,4,5-trihydroxybenzoic acid (gallic acid); (2) trans 3,4?,5-trihydroxystilbene (trans -resveratrol); (3) 3,3?,4?,5,7-pentahydroxyflavone (quercetin) and its glycoside (4) 3,3?,4?,5,7-pentahydroxyflavone Á/3-rutinoside (Rutin) have been subjected to antioxidant study by a fluorimetric assay. In this method, the rate of peroxidation induced by 2,2?-azobis (2-methylpropionamidine) dihydrochloride was monitored through the loss of fluorescence of the protein B-phycoerythrin (B-PE). Under appropriate conditions, the loss of B-PE fluorescence in the presence of reactive species is an index of oxidative damage of the protein. The inhibition of the action of reactive species by phenolic compounds, reflected in the protection against the loss of B-PE fluorescence in the fluorimetric assay, is a measure of its antioxidant capacity against the reactive species. The antioxidant effects of phenolic compounds have been investigated at different concentrations to relate activity to structural effects. It has been observed that the fluorescence decay due to peroxyl radical attack on B-PE decreases exponentially with time. As a reference compound for antioxidant capacity we used 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylicacid (trolox), a water soluble tocopherol analogue. This compound reacts rapidly with peroxyl radicals, and, until the trolox is consumed, no loss in phycoerythrin fluorescence is observed. A linear correlation of the net protection value with the concentration of trolox was demonstrated. The phenolic compounds studied react with peroxyl radicals in a similar way to trolox. Quercetin and rutin were shown to have strong antioxidant activities. The results obtained here are in agreement with previous studies confirming that quercetin is the most antioxidant of the four polyphenolics. #

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“…Pomegranate (Punica granatum L.) is known to possess pharmacological activities, such as antioxidant and anticancer [12][13][14]. In this study, we evaluated the antioxidant potency of a methanolic pomegranate fruit peel extract (PPE) and the relation with its antiproliferative and apoptotic effects on MCF-7human breast cancer cells [15,16] (Tables 1-3).…”

Section: Therapeutic Applicationsmentioning

confidence: 99%

Evaluating the Impact of Boosting on-site Enzymes produced by Trichoderma reesei RUT C30 and Aspergillus saccharolyticus with Commercial Enzymes in lowering the use of Commercial Cellulases.

Rana¹,

Ahring²

2015

Mol Enzyme Drug Targets

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In many countries of the world, cancer is one of the most serious health problems that affect the duration and quality of the individual's life. Enormous efforts are invested to cope with this problem, but unfortunately limited success has ever been achieved with most of the therapeutic strategies. These efforts are usually complicated with the need for well experienced surgeons, lack of specificity and high cost, as well as being usually accompanied with a wide range of side effects.As the conventional therapeutic strategies fail to fulfill the major requirements for a successful cancer therapy, the use of naturally developed anticancer agents has evolved as an alternative safe, low-cost and convenient one. Therefore, the use of plant extracts with potential anticancer therapeutic effects might be particularly significant. Therefore, the current study, investigates the effect of plant extracts from pomegranate (Punica granatum L. var spinosa) on breast cancer cell line (MCF-7).Results: Showed concentration-dependent reduction in the final number of cancer cells in consequence to treatment with the aforementioned plant extract. The anticancer effects were evaluated and contributed to the cytotoxic effects (decreased number of live cells). Conclusion:The activity of Punica granatum was good against cancer cell line .from this we concluded that PPE shows a better cancerous activity against the cancer as compare to PSE.

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Determination of phenolic acids in fruit juices by isocratic column liquid chromatography

Cited by 119 publications

References 8 publications

Simultaneous GC-MS Determination of Free and Bound Phenolic Acids in Slovenian Red Wines and Chemometric Characterization

Simultaneous GC-MS Determination of Free and Bound Phenolic Acids in Slovenian Red Wines and Chemometric Characterization

Study of phenolic compounds as natural antioxidants by a fluorescence method

Evaluating the Impact of Boosting on-site Enzymes produced by Trichoderma reesei RUT C30 and Aspergillus saccharolyticus with Commercial Enzymes in lowering the use of Commercial Cellulases.

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