Determination of phenolic compounds in extracts of Amazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometry

Faccin, Henrique; Loose, Roberta Fabricio; Viana, Carine; Lameira, O. A.; Carvalho, Leandro Machado de

doi:10.1039/c6ay02937j

Cited by 15 publications

(8 citation statements)

References 52 publications

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“…We used a mobile phase composed of acetonitrile gradients and 0.1% formic acid in water (v/v) which is typical in separating complex samples. [26,27] The developed method can be considered rapid as it allowed the separation of compounds at 254 nm in only 35 min. The optimized method was validated based on ICH guidelines.…”

Section: Discussionmentioning

confidence: 99%

An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves

Cavalcante¹,

Oliveira²,

Barreto³

et al. 2022

Pharmacogn. Res.

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Traditional communities in the Amazon region use a significant number of plant species for food and medical purposes. Nonetheless, many of these plants' chemical compositions, bioactive contents, biological properties, and medicinal efficacy are unknown. [1] In this context, Paula Filho (2018) [2] realized a phytochemical characterization of medicinal plants used by the population living on Cajari riverbanks in Amapá state. Among the studied plant species, the author reported the use of Acmella oleracea L., Byrsonima crassifolia (L.) Kunth, Cuminum cyminum L., Eryngium foetidum L., Inga edulis Mart., Pterocarpus rohrii Vahl, Senna alata (L.) Roxb and Talinum paniculatum (Jacq.), leaves. Previous studies have investigated extraction methods, identification, isolation, toxicity, bioactive efficacy, and applications of Amazonian plant species. [3][4][5] Silva et al.

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Section: Discussionmentioning

confidence: 99%

An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves

Cavalcante¹,

Oliveira²,

Barreto³

et al. 2022

Pharmacogn. Res.

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“…The MS detection was performed on a Qtrap 6500 MS (AB Sciex, Framingham, MA, USA) equipped with an ESI source operating in negative-ion mode. Quantification of 9 metabolites was carried out in the MRM mode [ 30 , 31 ]. At the same time, optimization to voltage (DP), cluster collision (CE) and mass spectrum parameters, such as the optimal conditions for 9 types of compound ion pairs for rapid screening, was performed.…”

Section: Methodsmentioning

confidence: 99%

Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear

Abdullah

et al. 2018

IJMS

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To investigate the effect of pollination on the fruit quality of ‘Dangshan Su’ pear, ‘Dangshan Su’ was fertilized by the pollen of ‘Wonhwang’ (Pyrus pyrifolia Nakai.) (DW) and ‘Jingbaili’ (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantitative analysis of intermediate metabolites of lignin synthesis was undertaken using targeted metabolomics. The untargeted metabolomics analysis was performed via gas chromatography-mass spectrometry (GC-MS). The targeted metabolomics analysis was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring (MRM) mode. The results showed that the metabolite content was significantly different between DW and DJ. Compared with that in DJ, the sugar and amino acid content in DW was higher and the fatty acid content was lower at 47 days after pollination (DAPs), and the sugar, amino acid, and fatty acid content in DW was lower at 63 DAPs. The intermediate metabolites of lignin synthesis were analyzed using the orthogonal partial least squares discriminant analysis (OPLS-DA) model, and the differential metabolites at 47 DAPs were p-coumaric acid, ferulic acid, sinapaldehyde, coniferyl alcohol, and sinapyl alcohol. The differential significant metabolite at 63 DAPs was p-coumaric acid. At 47 DAPs and 63 DAPs, the p-coumaric acid level was significantly different, and the p-coumaric acid content was positively correlated with lignin synthesis. The pollination pollen affects the quality of ‘Dangshan Su’ pear fruit through regulation of the sugar, amino acid, and fatty acid content; at the same time, regulating the levels of intermediate metabolites of lignin synthesis, especially the p-coumaric acid content, to affect lignin synthesis ultimately affects the stone cell content and improves the quality of the pears.

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“…In the Amazon region considered the most biodiverse region of the planet , numerous plant species have been the subject of phytochemical studies aimed at identifying their mineral composition, the concentration rate of primary and secondary metabolites, and their activities. In this context, several scientific articles have been published with these objectives (FACCIN et al, 2017;SILVEIRA et al, 2015;RAMOS et al, 2017).…”

Section: Capítulo 60mentioning

confidence: 99%

Phytochemistry of Medicinal Plants of the Amazon

Lameira¹,

Cordeiro²,

Pacheco³

et al. 2023

Methodology Focused on the Area of Interdisciplinarity- V1

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The results of the phytochemical research involving medicinal plants of the Amazon traditionally used by the population are described in this chapter as Pilocarpus microphyllus, Copaifera sp, Carapa guianensis, and Bauhinia sp, as well as qualitative and quantitative analysis of the phenolic content of Connarus perrottetti, Cecropia obtusa, Cecropia palmata, and Mansoa alliacea. A method was developed and validated to characterize the presence of phenolic compounds in extracts of medicinal plants from the Amazon for different years of sampling. A complete evaluation of the different possible causes of ion suppression in the analysis of extracts of medicinal plants by UHPLC-MS / MS using electrospray ionization is shown. The mineral content of dry matter composition of Cecropia palmata and Cecropia obtusa at different seasons evaluated the nutritional properties of the tea produced by infusion of the leaves of these two species of Cecropia. In the phytochemical analysis carried out in some species, the presence of sugar, protein, amino acid reducers, foamy saponin, and tannins was revealed. All of these results are part of this chapter.

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Determination of phenolic compounds in extracts of Amazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometry

Abstract: A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated.

Cited by 15 publications

References 52 publications

An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves

An HPLC Method to Determine Phenolic Compounds of Plant Extracts: Application to Byrsonima crassifolia and Senna alata Leaves

Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear

Phytochemistry of Medicinal Plants of the Amazon

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