Determination of Phenolic Contents by HPLC, and Antioxidant, Antimicrobial, Antityrosinase, and Anticholinesterase Activities of Psephellus huber-morathii

Abstract

The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. The antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and Korkmaz et al.; JPRI, 26(1): 1-10, 2019; Article no.JPRI.47659 2 antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. The methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity. Original Research Article

Exploring the Potential of Psephellus huber-Marathi (Wagenitz) Wagenitz: A Comprehensive UHPLC-MS/MS Analysis of Phytochemical Composition and Evaluation of Antioxidant, Antimicrobial, and Antiproliferative Activities

“…The rich phytochemical profile of the hydroalcoholic extract better reflects the antioxidant ability, TPC, and TFC of the plant. The antioxidant activity and TPC findings in this study support previous findings about PH (Sarker et al, 2005;Korkmaz et al, 2019).…”

“…Besides the major compounds, PH contains high amounts of quercetin-3-glucuronide (9.04 mg analyte g extract -1 ), isoquercitrin (6.62 mg analyte g extract -1 ), quercitrin (6.82 mg analyte g extract -1 ) and apigenin (2.43 mg analyte g extract -1 ). Two HPLC studies were reported for the phenolic compounds of PH (Korkmaz et al, 2019;Shoeb et al, 2007). Korkmaz et al identified 7 compounds in the methanol extract of the plant and quantified two of them.…”

“…In the literature, the antimicrobial activity of PH was investigated by Korkmaz et al (2019), the methanol extract of PH showed moderate antimicrobial activity against S. aureus, P. aeruginosa, and E. coli. (MIC values 100-500 µg mL -1 ) However, it was reported that the methanol extract has weaker activity against L. monocytogenes (Korkmaz et al, 2019). In the study, it was determined that the hydroalcoholic extract showed moderate activity against L. monocytogenes.…”

“…In addition, the essential oil profiles of the seeds were determined (Başer et al, 2006). However, there is only one study examining the total phenolic content (TPC), antioxidant, antimicrobial, antityrosinase, and anticholinesterase activities of the methanol extract of PH aerial parts (flowers, leaves, stem, and roots) (Korkmaz et al, 2019). Several studies reported the phytochemical compositions, and antioxidant, cytotoxic, and anti-inflammatory activities of other Psephellus species (Shoeb et al, 2007;Zengin et al, 2010;Polatoglu et al, 2015;Demiroz, Nalbantsoy, Kose, & Baykan, 2020).…”

Phenolic Content and Enzyme Inhibition Activities Barbarea auriculata var. paludosa, B. integrifolia and B. plantaginea (Brassicaceae)'s

“…Signals were identified using DAD detection at a column temperature of 25ºC. HPLC analyses were performed modified methods in our previous study [17,18].…”