Determination of phosphate concentration in glaucoma eye drops commercially available in Spain

Martínez-Soroa, Itziar; Frutos-Lezaun, M. de; Ostra, Miren; Zurutuza, Ane; Larburu, M.B. Irastorza; Cacho, M.P. Bachiller

doi:10.1016/j.oftale.2016.04.028

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…The formulations that were classified as "without phosphates" (< 0.6 ppm) were EDF 1, EDF 5, and EDF 9; whereas EDF 3 was the only formulation considered "phosphate-free" (< 0.3 ppm). These results demonstrate the substantial variability www.nature.com/scientificreports/ in phosphate concentration among the antiallergic formulations under study, that could not be correlated with the presence or absence of BAC, nor to the delivery system of the formulation (single-dose or multidose), as reported previously 54 . The present study also investigated the cytotoxic effect on conjunctival and corneal epithelial cells of the antiallergic formulations, determined by the MTT assay.…”

Section: Discussionsupporting

confidence: 73%

The new preservative-free ophthalmic formulation of bilastine 0.6% preserves the ocular surface epithelial integrity in a comparative in vitro study

Arana,

Gonzalo,

Andollo

et al. 2024

Sci Rep

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Allergic conjunctivitis (AC) is the most common form of allergic eye disease and an increasingly prevalent condition. Topical eye drop treatments are the usual approach for managing AC, although their impact on the ocular surface is not frequently investigated. The aim of this study was to perform a comparative physicochemical characterization, and in vitro biological evaluations in primary conjunctival and corneal epithelial cells of the new multidose preservative-free bilastine 0.6% and main commercially available eye drops. MTT assay was used to measure cell viability; oxidative stress was analyzed with a ROS-sensitive probe; and apoptosis was evaluated monitoring caspase 3/7 activation. Differences in pH value, osmolarity, viscosity and phosphate levels were identified. Among all formulations, bilastine exhibited pH, osmolarity and viscosity values closer to tear film (7.4, 300 mOsm/l and ~ 1.5–10 mPa·s, respectively), and was the only phosphates-free solution. Single-dose ketotifen did not induce ROS production, and single-dose azelastine and bilastine only induced a mild increase. Bilastine and single-dose ketotifen and azelastine showed high survival rates attributable to the absence of preservative in its formulation, not inducing caspase-3/7-mediated apoptosis after 24 h. Our findings support the use of the new bilastine 0.6% for treating patients with AC to preserve and maintain the integrity of the ocular surface.

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Section: Discussionsupporting

confidence: 73%

The new preservative-free ophthalmic formulation of bilastine 0.6% preserves the ocular surface epithelial integrity in a comparative in vitro study

Arana,

Gonzalo,

Andollo

et al. 2024

Sci Rep

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show abstract

“…Izatez, fosfatoa hidroxiapatita [Ca 5 (PO 4 ) 3 (OH)] kristal moduan hauspeatzen dira. Fosfato tanpoiek eta pH-aren igoerak kristal honen eraketa handiagotzen dute, eta ondorioz, kornearen kaltzifikazioak [30].…”

Section: Dia Eta Medikuntza: Malko Artifizialen Analisiaunclassified

Irudi Digitalen Analisia: espektrofotometroa ordezkatzen

Albizu

Berasarte²,

Eizaguirre³

et al. 2021

EKAIA

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Irudi digitalen analisia (DIA) teknika multzo batek osatzen du eta helburu nagusia aztergai den sistema bati buruzko informazio adierazgarria determinatzea da sistema horren irudiak erabiliz. Metodoaren abantaila nagusiak bere abiadura, sinpletasuna eta beharrezko lagin kopuru txikia dira (400 μL-tik behera mikroplakak erabiltzen badira), baita sortutako hondakin kopuru txikia ere. Lan honetan DIA-ren hiru aplikazio desberdin deskribatzen dira: nikel bainuetan aurki daitezkeen konposatu nagusien analisia eta kontrola, hala nola, nikela, amonioa eta sulfatoak; fosfatoen eta pH-aren aldibereko determinazioa malko artifizialetan eta tartrazina eta allura gorria koloratzaileen determinazioa jangaietan. Aplikazio hauetan, mahai-gaineko eskanerra eta telefono mugikorra erabili dira irudiak eskuratzeko.

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“…Hau horrela, fosfatoa hidroxiapatita [Ca5(PO4)3(OH)] kristal moduan hauspeatzen da. Fosfato tanpoiek eta pH-aren igoerak kristal honen eraketa handiagotzen dute, eta ondorioz, kornearen kaltzifikazioak (Martínez-Soroa et al, 2016).…”

Section: Sarrera Eta Motibazioaunclassified

“…Egoera arrunt batean fosfato kontzentrazioa oso txikia da; 1,45 mM ingurukoa . Epe motzez erabiltzen diren medikamentuen kasuan eszipienteekin dagoen kontaktua minimoa da, baina gaixotasun jakin batzuetan eguneroko pilaketek eszipiente kontzentrazioa nabarmenki handitu dezakete, kaltzio fosfato hauspeakinak areagotuz (Martínez-Soroa et al, 2016). Arazo hau saihesteko beharrezkoa da begi-tantetan dagoen fosfato kontzentrazio zehatza ezagutzea.…”

Section: Sarrera Eta Motibazioaunclassified

IV. Ikergazte. Nazioarteko ikerketa euskaraz. Kongresuko artikulu bilduma. Zientziak eta Natura Zientzia

2021

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Determination of phosphate concentration in glaucoma eye drops commercially available in Spain

Cited by 6 publications

References 23 publications

The new preservative-free ophthalmic formulation of bilastine 0.6% preserves the ocular surface epithelial integrity in a comparative in vitro study

The new preservative-free ophthalmic formulation of bilastine 0.6% preserves the ocular surface epithelial integrity in a comparative in vitro study

Irudi Digitalen Analisia: espektrofotometroa ordezkatzen

IV. Ikergazte. Nazioarteko ikerketa euskaraz. Kongresuko artikulu bilduma. Zientziak eta Natura Zientzia

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