Determination of phosphatidylethanol 16:0/18:1 in whole blood by 96‐well supported liquid extraction and <scp>UHPLC</scp>‐<scp>MS</scp>/<scp>MS</scp>

Berg, Thomas; Eliassen, Elin; Jørgenrud, Benedicte; Kabashi, Saranda; Петухов, А. Е.; Bogstrand, Stig Tore

doi:10.1002/jcla.22631

Cited by 19 publications

(4 citation statements)

References 53 publications

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“…The samples were analyzed for PEth 16:0/18:1 using the validated UHPLC-MS/MS method described by Berg et al. ( 2019 ). This included sample extraction using Solid Liquid Extraction (SLE) plates Isolute 96-well SLE+ plate with 400 μl bed volume from Biotage (Uppsala, Sweden), using 100 μl blood samples from patients, and calibrators and control samples were prepared by using working solutions added to PEth-free whole blood.…”

Section: Methodsmentioning

confidence: 99%

The Association between the Alcohol Biomarker Phosphatidylethanol (PEth) and Self-Reported Alcohol Consumption among Russian and Norwegian Medical Patients

Jørgenrud

Kabashi

Nadezhdin

et al. 2021

Alcohol and Alcoholism

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Aims Valid measures to identify harmful alcohol use are important. Alcohol Use Disorders Identification Test (AUDIT) is a validated questionnaire used to self-report harmful drinking in several cultures and settings. Phosphatidylethanol 16:0/18:1 (PEth) is a direct alcohol biomarker measuring alcohol consumption levels. The aim of this study was to investigate how PEth levels correlate with AUDIT-QF and weekly grams of alcohol consumed among patients in two urban hospitals. In addition, we wanted to investigate the predictive value of PEth in identifying harmful alcohol use as defined by AUDIT-QF and weekly grams of alcohol cutoffs. Methods A cross-sectional study comprising acute medically ill patients with measurable PEth levels (≥0.030 μM) admitted to two urban hospitals in Oslo, Norway (N = 931) and Moscow, Russia (N = 953) was conducted using PEth concentrations in whole blood, sociodemographic data and AUDIT-QF questionnaires. Results PEth levels from patients with measurable PEth were found to be positively correlated with AUDIT-QF scores, with PEth cutpoints of 0.128 μM (Oslo) and 0.270 μM (Moscow) providing optimal discrimination for harmful alcohol use defined by AUDIT-QF (the difference between cities probably reflecting different national drinking patterns in QF). When converting AUDIT-QF into weekly grams of alcohol consumed, the predictive value of PEth improved, with optimal PEth cutpoints of 0.327 (Oslo) and 0.396 (Moscow) μM discriminating between harmful and non-harmful alcohol use as defined in grams (≥350 grams/week). Conclusions By using PEth levels and converting AUDIT-QF into weekly grams of alcohol it was possible to get an improved rapid and sensitive determination of harmful alcohol use among hospitalized patients.

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Section: Methodsmentioning

confidence: 99%

The Association between the Alcohol Biomarker Phosphatidylethanol (PEth) and Self-Reported Alcohol Consumption among Russian and Norwegian Medical Patients

Jørgenrud

Kabashi

Nadezhdin

et al. 2021

Alcohol and Alcoholism

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“…PEth assayed by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) has been reported using various specimen preparations of whole blood including in liquid whole blood, dried blood spots (DBS) and isolated erythrocytes 20–22 . Proficiency testing, by means of participation in an external quality assurance program (QAP) or by inter‐laboratory comparisons is required for accrediting a clinical diagnostic laboratory test to international standards 23,24 .…”

Section: Introductionmentioning

confidence: 99%

LC‐MS/MS analysis of erythrocyte phosphatidylethanol in haematocrit‐corrected whole blood versus isolated erythrocytes: Results of an inter‐laboratory comparison

White

Fitzpatrick

McWhinney

et al. 2023

Drug Testing and Analysis

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Phosphatidylethanol (PEth) is a non‐oxidative metabolite of alcohol (ethanol), which is a sensitive and specific indicator of historic ethanol consumption. Although PEth production from ethanol is catalysed by the ubiquitous enzyme phospholipase D, it resides mainly within the erythrocyte compartment of the blood. PEth analysis has been reported in different preparations of whole blood, representing one of the barriers of inter‐laboratory comparisons. We previously reported that expressing PEth concentrations in terms of blood erythrocyte content is more sensitive than whole blood volume, and haematocrit‐corrected liquid whole blood calculations of erythrocyte PEth and isolated erythrocyte PEth concentrations are comparable when assayed under identical analytical conditions. Acceptance of a clinical diagnostic assay by accreditation bodies requires proficiency testing with a third‐party analytical facility. To explore different blood preparations within the same inter‐laboratory program, 60 matched isolated erythrocyte or liquid whole blood specimens were tested at three laboratories. Laboratories measured PEth by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS), two using isolated erythrocytes, while the third used liquid whole blood, which underwent haematocrit correction before comparison with isolated erythrocyte PEth concentrations. There was acceptable consensus (87%) among laboratories to detect PEth around a cut‐off of 35 μg/L of erythrocytes. Each laboratory correlated well with the group average PEth concentration (R > 0.98) for each specimen above the cut‐off. Differences were observed between laboratories in bias, which did not affect comparable sensitivity at the selected cut‐off. This work demonstrates the feasibility of an inter‐laboratory comparison for erythrocyte PEth analysis across different LC‐MS/MS methodologies and different blood preparations.

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“…2,12 Several methods are described for the single or combined detection of alcohol biomarkers in blood. 10,11,[14][15][16][17][18][19] Until now, the development of an analytical method for the simultaneous detection of all four alcohol biomarkers (EtG, EtS, NAcT, and PEth) in blood was not pursued.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an analytical method for the simultaneous determination of the alcohol biomarkers ethyl glucuronide, ethyl sulfate, N‐acetyltaurine, and 16:0/18:1‐phosphatidylethanol in human blood

Hofmann

Sundermann

Schmitt

et al. 2021

Drug Testing and Analysis

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As alcohol is the most common addictive substance worldwide, it is inevitable to advance the established research. New and more substantial analytical methods can be applied to reply to complex questions in legal or forensic contexts. Therefore, an analytical method for the simultaneous determination of four different alcohol biomarkers-ethyl glucuronide, ethyl sulfate, N-acetyltaurine, and 16:0/18:1-phosphatidylethanol-in human blood was developed, validated, and verified. Despite the different chemical properties of the analytes, a specific determination via HPLC-MS/MS was achieved using a novel type of a Phenomenex Luna ® Omega Sugar column. Furthermore, all criteria for a successful validation were fulfilled according to forensic guidelines. The method proved to be linear and demonstrates selectivity and sufficient sensitivity for every biomarker. LODs obtained with this method of 2.6 ng/ml (EtG), 4.7 ng/ml (EtS), 12.5 ng/ml (NAcT), and 6.9 ng/ml (PEth) were in an acceptable range for routine applications, and the stability of all analytes over a range of 12 h is given. The verification of the new developed method was performed with authentic samples. Thus, whole blood and postmortem samples were analyzed to obtain information about the drinking behavior, which can answer complex questions regarding alcohol consumption.

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Determination of phosphatidylethanol 16:0/18:1 in whole blood by 96‐well supported liquid extraction and UHPLC‐MS/MS

Cited by 19 publications

References 53 publications

The Association between the Alcohol Biomarker Phosphatidylethanol (PEth) and Self-Reported Alcohol Consumption among Russian and Norwegian Medical Patients

The Association between the Alcohol Biomarker Phosphatidylethanol (PEth) and Self-Reported Alcohol Consumption among Russian and Norwegian Medical Patients

LC‐MS/MS analysis of erythrocyte phosphatidylethanol in haematocrit‐corrected whole blood versus isolated erythrocytes: Results of an inter‐laboratory comparison

Development and validation of an analytical method for the simultaneous determination of the alcohol biomarkers ethyl glucuronide, ethyl sulfate, N‐acetyltaurine, and 16:0/18:1‐phosphatidylethanol in human blood

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