Determination of phospholipids in milk samples by means of hydrophilic interaction liquid chromatography coupled to evaporative light scattering and mass spectrometry detection

Donato, Paola; Cacciola, Francesco; Cichello, Filomena; Russo, Marina; Dugo, Paola; Mondello, Luigi

doi:10.1016/j.chroma.2011.07.036

Cited by 113 publications

(103 citation statements)

References 37 publications

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“…1). The elution order observed for PL classes is typical of HILIC separations performed on a conventional silica stationary phase [25,26,28,32,34,35]. However, replacing conventional column packings with porous particles such as fusedcore silica (2.7 μm) leads to significantly improved chromatographic resolution, at least for some PL classes.…”

Section: Resultsmentioning

confidence: 93%

“…4). Indeed, although four different chromatographic peaks were observed in the ESI(+)-MS XIC trace, four (34,36,37,39) and two (35,38) peaks were found when analyzing the ESI(−) XIC traces corresponding to a m/z shift of −16.0308 and −38.0127 Da, respectively (see panels B and C). This finding was interpreted with the presence of four [M+H] + and two [M+Na] + adduct ions; unfortunately, two peaks due to protonated species were co-eluting with the sodiated ones (36 with 35 and 39 with 38), as shown in Fig.…”

Section: Protonated and Sodiated Forms Of Lpcmentioning

confidence: 95%

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Identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (Sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution Fourier-transform mass spectrometry

et al. 2015

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The numerous and varied biological roles of phosphatidylcholines (PC) and lysophosphatidylcholines (LPC) have fueled a great demand for technologies that enable rapid, in-depth structural examination of these lipids in foodstuffs. Here, we describe the capabilities of a newly configured combination of high-efficiency liquid chromatography and high-resolution/accuracy Fourier-transform mass spectrometry with electrospray ionization (LC-ESI-FTMS), designed for lipidomics applications that require the identification of PC in their lyso forms. The devised strategy, involving a separation by hydrophilic interaction liquid chromatography (HILIC) on spherical, fused-core ultrapure silica particles (2.7 μm) of a narrow-bore column (2.1 mm i.d.), enabled the identification of as many as 71 LPC species in the lipid extracts of gilthead sea bream (Sparus aurata) fillets. In this way, LPC as proton (43) and sodium (28) adducts, i.e., [M + H](+) and [M + Na](+) ions (with M representing the zwitterionic form), were identified. In several cases, the extremely high (sub-ppm) mass accuracy and the high chromatographic efficiency available with the adopted instrumentation enabled the distinction of isobaric and closely eluting LPC species. Informative tandem mass spectra, based on high-energy collision induced dissociation (HCD), were also obtained, thus distinguishing regioisomeric LPC species (i.e., LPC differing only for the location of the residual side chain on the glycerol backbone) and between proton and sodium adducts. Graphical Abstract Extracted Ion Current chromatogram (XIC) obtained for the m/z value 568.339, showing the presence of two regioisomeric Lysophosphatidylcholines. The corresponding high collisional energy tandem MS spectra, obtained using a HCD cell, are shown as insets.

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Section: Resultsmentioning

confidence: 93%

Section: Protonated and Sodiated Forms Of Lpcmentioning

confidence: 95%

Identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (Sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution Fourier-transform mass spectrometry

et al. 2015

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“…The phospholipids (PL) present in cashews were identified using methods adapted from Donato et al (2011) and Lee, Welti, Schapaugh, & Trick (2011). PL were extracted from the expressed oils (n=3) using 2:1 (vol/vol) chloroform/methanol 3 times and dried to a volume of 1 mL of solvent for 3 hours using a TurboVap (Biotage, Uppsala, Sweden).…”

Section: Determination Of Phospholipidsmentioning

confidence: 99%

Nutrient Composition of Raw, Dry-Roasted, and Skin-On Cashew Nuts

Griffin

Dean

2017

JFR

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Cashew nuts are the second most popular tree nut in the US with sales growing at a rate of 7% per annum. The highest quality cashew nuts are traditionally whole, oil-roasted, and devoid of skins. The development of a technique to remove the caustic cashew nut shell liquid from cashews and leave the skins intact allows for the production of novel cashew products including skin-on or "wrapped" in addition to raw and dry roasted products. This study investigated the nutritional characteristics of these newer cashew products. These products were found to contain bioactive compounds including mono-and poly-unsaturated fatty acids, phytosterols, arginine, magnesium, tocopherols, and phenolic compounds. All the types of cashews exhibited higher levels of phytosterols than the amounts reported for other tree nuts. The skin-on cashews had higher levels of phenolic compounds compared to the other cashew varieties tested, indicating additional health benefits of consuming cashew nuts with skins.

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“…Unlike monogastric animals, the microflora in the rumen is able to hydrogenate most of the unsaturated fatty acids introduced by feeding, increasing saturated lipids 5 . Several studies report the positive bio-logical activities of food phospholipids on human health 6,7 by virtue of both lipophilic and hydrophilic properties 8,9 . The phospholipid content in beef muscle ranged between 0.5 and 0.6 g/100 g of fresh muscle 10 .…”

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confidence: 99%

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

Marzocchi

Pasini

Baldinelli³

et al. 2018

J. Oleo Sci.

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Determination of phospholipids in milk samples by means of hydrophilic interaction liquid chromatography coupled to evaporative light scattering and mass spectrometry detection

Cited by 113 publications

References 37 publications

Identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (Sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution Fourier-transform mass spectrometry

Identification of isobaric lyso-phosphatidylcholines in lipid extracts of gilthead sea bream (Sparus aurata) fillets by hydrophilic interaction liquid chromatography coupled to high-resolution Fourier-transform mass spectrometry

Nutrient Composition of Raw, Dry-Roasted, and Skin-On Cashew Nuts

Value-addition of Beef Meat By-products: Lipid Characterization by Chromatographic Techniques

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