Determination of phycobiliproteins by constant-wavelength synchronous spectrofluorimetry method in red algae

Yusty, María Asunción Lage; Adán, Patricia Caramés; López-Hernández, J.

doi:10.1080/19476337.2012.728629

Cited by 14 publications

(2 citation statements)

References 28 publications

(48 reference statements)

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“…As propriedades da biomassa de Spirulina vão muito além da nutrição, seja humana ou vegetal, apresenta em seu conteúdo algo em torno de 15% das ficobiliproteínas, complexo proteico formado por (ficocianina, ficoeritrina e aloficocianina) (Pentón-Rol et al, 2018) que é responsável pelo aumento de imunidade em animais e plantas. Possui ainda inúmeros componentes peptídicos pouco ainda estudados, que são responsáveis pela sinalização dos processos celulares (Lage-Yusty et al, 2013;Cuellar-Bermudez et al, 2015;Lisboa & Costa, 2016;Yu et al, 2016;Saboya et al, 2017) Por fim, a Spirulina é um organismo que tem muitas aplicabilidades no que se refere a tecnologia agroalimentar.…”

Section: Introductionunclassified

Revestimento biodegradável à base de Spirulina platensis na conservação pós-colheita de goiaba Paluma mantidas sob diferentes temperaturas de armazenamento

Onias¹,

Teodosio²,

Bomfim³

et al. 2018

Rev. Ciênc. Agr.

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Spirulina platensis é uma microalga rica em proteínas que apresenta composição apropriada para utilização como complemento alimentar.. O objetivo deste trabalho foi avaliar diferentes concentrações de Spirulina platensis como revestimento biodegradável na conservação pós-colheita de goiaba 'Paluma' sob armazenamento refrigerado e ambiente. O ensaio foi desenvolvido na Universidade Federal de Campina Grande, Centro de Ciências e Tecnologia Agroalimentar e instalado em delineamento inteiramente casualizado, em esquema fatorial 2 x 4 x 5, utilizando dois tipos armazenamento (a 10 ± 2°C, com 85±5%UR e a 24 ± 2°C, com 85±5% UR), quatro concentrações da microalga (0%, 1%, 2% e 3%) e cinco períodos de avaliação (0, 3, 6, 9 e 12 dias). Os resultados mostraram que os revestimentos com Spirulina platensis foram eficientes no retardo da perda de massa nos frutos em ambas as temperaturas de armazenamento, sendo o tratamento T2, que se refere a concentração de 1% a 10±2°C o mais eficiente, proporcionando as menores perdas quando comparado a testemunha. Quanto às demais características de qualidade do fruto não foram observadas influências significativas dos revestimentos.

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Section: Introductionunclassified

Revestimento biodegradável à base de Spirulina platensis na conservação pós-colheita de goiaba Paluma mantidas sob diferentes temperaturas de armazenamento

Onias¹,

Teodosio²,

Bomfim³

et al. 2018

Rev. Ciênc. Agr.

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“…Phycobilin, a water-soluble protein with a wide range of applications has stirred great interest recently as these non-toxic chromoproteins have anti-inflammatory, radical scavenging, antioxidant and hepato-protective properties [ 2 , 3 , 4 ]. Owing to their numerous benefits, phycobiliproteins have been extensively utilized as colorants in food or used in the pharmaceutical and cosmetics industries [ 5 , 6 , 7 ] They are classified into three major groups based on their absorption and spectroscopic properties, namely, phycocyanins (PC), phycoerythrins (PE) and allophycocyanins (APC) [ 6 , 8 ]. Among these three types of phycobiliproteins, PC made up to 20% of all the proteins in cyanobacteria [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins from Arthrospira sp.

et al. 2020

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The freezing–thawing method had been reported to be the best phycobiliprotein extraction technique. However, optimum parameters of this extraction method for Arthrospira sp. (one of the major phycobiliprotein sources) still remained unclear. Hence, this study aimed to optimize the freezing–thawing parameters of phycobiliprotein extraction in Arthrospira sp. (UPMC-A0087). The optimization of the freezing–thawing method was conducted using different solvents, biomass/solvent ratios, temperatures, time intervals and freezing–thawing cycles. The extracted phycobiliproteins were quantified using a spectrophotometric assay. Double distilled water (pH 7) with a 0.50% w/v biomass/solvent ratio was the most efficient solvent in extracting high concentrations and purity of phycobiliproteins from Arthrospira sp. In addition, the combination of freezing at −80 °C (2 h) and thawing at 25 °C (24 h) appeared to be the optimum temperature and extraction time to obtain the highest amount of phycobiliproteins. A minimum of one cycle of freezing and thawing was sufficient for extracting high concentrations of phycobiliproteins. The findings from this study could reduce the cost and labor needed for extracting high quality phycobiliproteins. It also allowed the harvesting of large amounts of valuable phycobiliproteins.

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Biophotonics for improving algal photobioreactor performance: A review

et al. 2020

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The use of algae as a feedstock for biofuels production has drawn considerable attention due to their high biomass yield, their ability to be cultivated using degraded water on nonarable land, and their ability to recover nutrients from wastewater. Although algae have the potential to provide biomass for biofuels, some challenges remain and the limitations may be overcome by improving algal growth rates together with lipid synthesis. To achieve this, scientific researchers have focused on isolating and screening algal strains with better growth rates and lipid synthesis capabilities, bioengineering, and optimizing culture systems. The present review focuses on the biophotonic-based manipulations that can be applied to optimize solar-powered photobioreactors (PBRs).Hence, three different types of solar filters are reviewed herein, that is, the colored glass, thin-film, and thermochromic filters. This review provides evidence that bright red-colored glass filters can lower the spectral intensity of solar radiation from 1982.13 to 393.71 μmol m −2 s −1 , which is preferable for improved biomass productivity. Changing filter color, once the desired biomass concentration has been amassed, to medium blue or bright pink further improves lipid yield. A 34% improvement in biomass productivity was observed for Chlorella vulgaris cultured under thin-film filters. Thin-film filters are also effective in regulating PBR temperature within the 24-31 C range, which is tolerable for most algal species. Furthermore, this study highlights that the applicability of thermochromic filters in PBR designs is still yet to be investigated. Thermochromic filters are reflective and absorptive under high and low temperatures, respectively, a technology that can be a solution to the overheating challenge of PBRs. This review recommends the coupling of these light

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Determination of phycobiliproteins by constant-wavelength synchronous spectrofluorimetry method in red algae

Cited by 14 publications

References 28 publications

Revestimento biodegradável à base de Spirulina platensis na conservação pós-colheita de goiaba Paluma mantidas sob diferentes temperaturas de armazenamento

Revestimento biodegradável à base de Spirulina platensis na conservação pós-colheita de goiaba Paluma mantidas sob diferentes temperaturas de armazenamento

Optimization of the Freezing-Thawing Method for Extracting Phycobiliproteins from Arthrospira sp.

Biophotonics for improving algal photobioreactor performance: A review

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