Determination of Physiological, Biochemical and Molecular Effects of Zinc Stress on the Growth of Sunflower Seedlings (Helianthus annuus L.)

Bolukbasi, Ekrem; Kayabaş, Ayşenur; Aras, Sümer

doi:10.22161/ijeab/3.2.29

Cited by 3 publications

(9 citation statements)

References 7 publications

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“…The toxic effect of zinc caus e damages to the cell division and it especially gives damages to the cell nucleus of meristematic stem cellas cell division. Also genetic variations in sensitivity to Zn toxicity has been mapped in plants [10,12]. Zinc (Zn) is a potential environmental toxicant for plants under excessive conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of Zinc Heavy Metal on Stress-Related Genes in Tomato (Solanumlycopericum L.) Plants

Bolukbasi¹,

Karakaş²

2019

IJEAB

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Heavy metal pollution is a major environmental problem all over the world. It is known that high concentration of heavy metals in soils and waters cause genotoxicity and damage to most of the functional biomolecules. The aim of this study was to determine the molecular changes in tomato ( Solanum lycopericum L.) genome under heavy metal of zinc stress. Zinc is a microelement which should be taken in very less amounts by plants, animals and humans. In plants, zinc in low concentration is essential for root and stem elongation, RNA levels, the cell's ribosome content and protein formation mechanism. But at high concentrations, it is toxic for plants like cadmium, lead and copper.In this current study, the molecular response of tomato (Solanum lycopericum L.) plants to zinc stress was examined by transcript accumulation analysis of two stress-related genes: (i) MT2 (metallothionein) gene, coding for a metal -binding protein and (ii) GR1 (glutathionreductase) gene, a marker of enzymatic ROS scavenging mechanism. A quantitative Real-Time PCR experiment was performed with MT2 and GR1 genes using RNA isolated from tomato roots or shoots treated for 24h with zinc at concentrations ranging from 20 to 1280ppm. Results showed that the genes were over-expressed in zinc-stressed tomato. The highest relative fold change value was measured on GR1 for both root and shoot indicating the activation of the oxidative stress enzyme to tolerate zinc stress.

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Section: Introductionmentioning

confidence: 99%

Effect of Zinc Heavy Metal on Stress-Related Genes in Tomato (Solanumlycopericum L.) Plants

Bolukbasi¹,

Karakaş²

2019

IJEAB

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“…Similarly, 4.2 and 4.7 fold changes were detected in shoots at 80 and 160 mg L-1 concentrations, respectively (Figure 3). It is cleared from the results obtained that the change in the expression of the metallothionein binding protein gene MT-2 can induce cadmium toxicity tolerance in tomato plant [7,29,32,33].…”

Section: Resultsmentioning

confidence: 99%

“…It plays a critical role in the defense system against ROS, which occurs as a result of stress factors, by maintaining the GSH level and acts as a substrate for glutathione-Stransferases. In many recent studies, it was stated that GR-1 expression increased against cadmium stress in various plants [7,9,[31][32][33][34][35].…”

Section: Resultsmentioning

confidence: 99%

“…This also shows that; it is the ineffectiveness of the resistance mechanism against cadmium stress in both roots and shoots after the specified concentrations. It was determined that the GR pathway was inhibited due to possible cellular damage and it could not provide protection against cadmium stress by using the stress recovery pathway [7,[31][32][33][34].…”

Section: Resultsmentioning

confidence: 99%

“…Glutathione reductase, an enzyme especially active in the ascorbate-glutathione (ASH-GSH) pathway, plays a role in defense against ROS by maintaining the low status of GSH [30]. By determining the changes in gene expression levels of metallothionein and glutathione reductase in plants, the tolerance of plants to various types of stress can be determined [29][30][31][32][33].…”

Section: First Strand Cdna Synthesis Assaymentioning

confidence: 99%

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Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Bolukbasi

2021

Hittite Journal of Science and Engineering

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T omato is an important fruit crop grown mainly as an annual and economically valuable plant for growers in the Mediterranean basin (Solanum lycopersicum L. formerly Lycopersicon esculentum Mill. belongs to the Solanaceae family). It is an important source of vitamins, minerals, fiber and a dietary antioxidants [1]. It is also among the anti-carcinogenic foods due to the carotenoids it contains. Consumed as fresh and dried fruit, tomatoes are also processed in industry as tomato paste. Turkey, Egypt, Italy, Spain, Greece and Morocco are among the world's largest tomato producers and exporters [2].With the increasing population, unplanned urbanization and developing technology, heavy metal pollution has become an important environmental problem worldwide. In addition to these, as a result of industrial activities, mining, using pesticide in agriculture, metallurgy, combustion of fossil fuels, faulty waste disposal, metal-enriched some materials, automotive emissions and domestic wastes and many other factors [3][4][5]. He-Article History:

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Modeling DNA Methylation Profiles and Epigenetic Analysis of Safflower (Carthamus tinctorius L.) Seedlings Exposed to Copper Heavy Metal

Bolukbasi

Karakaş

2023

Toxics

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Heavy metals are chemical elements with high density that can be toxic or poisonous even at low concentrations. They are widely distributed in the environment due to industrial activities, mining, pesticide use, automotive emissions and domestic wastes. This study aimed to investigate the toxic effects of copper (Cu) heavy metal on safflower plants in terms of genetic and epigenetic parameters. Safflower seeds were exposed to different concentrations of Cu heavy metal solution (20, 40, 80, 160, 320, 640, 1280 mg L−1) for three weeks, and changes in the genomic template stability (GTS) and methylation pattern in the root tissues were analyzed using PCR and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques. The results indicated that high doses of Cu have genotoxic effects on the genome of safflower plants. Epigenetic analysis revealed four different methylation patterns, with the highest total methylation rate of 95.40% observed at a 20 mg L−1 concentration, and the lowest rate of 92.30% observed at 160 mg L−1. Additionally, the maximum percentage of non-methylation was detected at 80 mg L−1. These results suggest that changes in the methylation patterns can serve as an important mechanism of protection against Cu toxicity. Furthermore, safflower can be used as a biomarker to determine the pollution in soils contaminated with Cu heavy metal.

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Determination of Physiological, Biochemical and Molecular Effects of Zinc Stress on the Growth of Sunflower Seedlings (Helianthus annuus L.)

Cited by 3 publications

References 7 publications

Effect of Zinc Heavy Metal on Stress-Related Genes in Tomato (Solanumlycopericum L.) Plants

Effect of Zinc Heavy Metal on Stress-Related Genes in Tomato (Solanumlycopericum L.) Plants

Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Modeling DNA Methylation Profiles and Epigenetic Analysis of Safflower (Carthamus tinctorius L.) Seedlings Exposed to Copper Heavy Metal

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